Molecular characterization of a centromeric satellite DNA in the hemiclonal hybrid frogRana esculenta and its parental species

Ragghianti, Matilde; Guerrini, Francesca; Bucci, Stefania; Mancino, Giorgio; Hotz, Hansjürg; Uzzell, Thomas; Guex, Gaston‐Denis

doi:10.1007/bf00713965

Cited by 51 publications

(55 citation statements)

References 32 publications

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“…The development of the germ cells in green frogs of the genus Rana has been thoroughly investigated by means of cytological, genetic and cytogenetic approaches (Uzzell et al 1980;Bucci et al 1990Bucci et al , 1999Ragghianti et al 1995;Ogielska & Kotusz 2004). Interesting information emerged from studies about Rana esculenta gametogenesis (Chieffi et al 2000;Ferrara et al 2004;Meccariello et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Isolation and expression ofRlYB2, a germ cell‐specificY‐boxgene inRana

Marracci

Casola

Bucci

et al. 2008

Italian Journal of Zoology

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Y-box proteins are a highly conserved family of gene expression regulatory factors. During gametogenesis they may play a dual role, as both transcriptional activators of germ cell-specific genes and as translational repressors of stored maternal transcripts. We report the identification of RlYB2, a Y-box homolog gene specifically expressed in the germ cells of green frogs belonging to Rana esculenta complex, a model system characterized by a hybridogenetic gametogenesis. In developing germ cells of the hybrid R. esculenta, arisen by natural cross of the parental species R. ridibunda and R. lessonae, one set of the parental genomes is excluded and the remaining one, first endoreduplicates and then is transmitted to gametes. In situ hybridizations performed on gonadal tissues showed that the RlYB2 transcript was widely expressed in the ooplasm at early stages of oogenesis in both the parental species and hybrids. Interestingly, a hybridization signal, presumably related to RlYB2-like nascent transcripts, was observed in nuclei of stage II oocytes. The presence of RlYB2 mRNA during early oogenesis suggests that this gene may be involved in regulating the transcription and/or translation of maternal mRNAs in this special vertebrate model system.

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Section: Discussionmentioning

confidence: 99%

Isolation and expression ofRlYB2, a germ cell‐specificY‐boxgene inRana

Marracci

Casola

Bucci

et al. 2008

Italian Journal of Zoology

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“…Mitotic and meiotic preparations were banded using Arrighi & Hsu's (1971) method for staining of heterochromatin. DIGlabelled DNA insert from pRr300 StuI, a clone of the centromeric satellite DNA family RrS1, was hybridized in situ to mitotic and meiotic chromosome preparations using standard procedures (Ragghianti et al, 1995).…”

Section: (I) Animals and Experimental Crossesmentioning

confidence: 99%

“…For Southern blot experiments, fragments of completely StuI-digested genomic DNA of all offspring were separated electrophoretically and then transferred (Southern, 1975) to Hybond-N filters (Amersham). Southern hybridizations were carried out as described by Ragghianti et al (1995).…”

Section: (I) Animals and Experimental Crossesmentioning

confidence: 99%

Gametogenesis of intergroup hybrids of hemiclonal frogs

et al. 2007

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European water frog hybrids Rana esculenta (R. ridibundarR. lessonae) reproduce hemiclonally, by hybridogenesis : in the germ line they exclude the genome of one parental species and produce haploid gametes with an unrecombined genome of the other parental species. In the widespread L-E population system, both sexes of hybrids (E) coexist with R. lessonae (L). They exclude the lessonae genome and produce ridibunda gametes. In the R-E system, hybrid males coexist with R. ridibunda (R); they exclude either their ridibunda or their lessonae genome and produce sperm with a lessonae or with a ridibunda genome or a mixture of both kinds of sperm. We examined 13 male offspring, 12 of which were from crosses between L-E system and R-E system frogs. All were somatically hybrid. With one exception, they excluded the lessonae genome in the germ line and subsequently endoreduplicated the ridibunda genome. Spermatogonial metaphases contained a haploid or a diploid number of ridibunda chromosomes, identified through in situ hybridization to a satellite DNA marker, and by spermatocyte I metaphases containing a haploid number of ridibunda bivalents. The exception, an F1 hybrid between L-E system R. lessonae and R-E system R. ridibunda, was not hybridogenetic, showed no genome exclusion, and evidenced a disturbed gametogenesis resulting from the combination of two heterospecific genomes. None of the hybridogenetic hybrids showed any cell lines excluding the ridibunda genome, the pattern most frequent in hybrids of the R-E system, unique to that system, and essential for its persistence. A particular combination of R-E system lessonae and R-E system ridibunda genomes seems necessary to induce the R-E system type of hemiclonal gametogenesis. Gametogenesis of intergroup hybrids of hemiclonal frogs SummaryEuropean water frog hybrids Rana esculenta (R. ridibundarR. lessonae) reproduce hemiclonally, by hybridogenesis : in the germ line they exclude the genome of one parental species and produce haploid gametes with an unrecombined genome of the other parental species. In the widespread L-E population system, both sexes of hybrids (E) coexist with R. lessonae (L). They exclude the lessonae genome and produce ridibunda gametes. In the R-E system, hybrid males coexist with R. ridibunda (R) ; they exclude either their ridibunda or their lessonae genome and produce sperm with a lessonae or with a ridibunda genome or a mixture of both kinds of sperm. We examined 13 male offspring, 12 of which were from crosses between L-E system and R-E system frogs. All were somatically hybrid. With one exception, they excluded the lessonae genome in the germ line and subsequently endoreduplicated the ridibunda genome. Spermatogonial metaphases contained a haploid or a diploid number of ridibunda chromosomes, identified through in situ hybridization to a satellite DNA marker, and by spermatocyte I metaphases containing a haploid number of ridibunda bivalents. The exception, an F1 hybrid between L-E system R. lessonae and R-E system R. ridibunda, w...

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“…4B). To our knowledge, the highly repetitive DNA sequences showing regions homologous to CDEIII have been reported in the Antarctic scallop Adamussium colbecki [31], in Lacerta graeca [32], in Drosophila melanogaster [33] and in Rana esculenta [34]. Since the CDEIII sequences in these DNA arrangements are always associated with the CENP-B box motifs, it has been hypothesized that the repetitive DNA sequences containing the centromere motifs might have a role in determining the centromere function.…”

Section: Sequence Organization and Intraspecific Variabilitymentioning

confidence: 98%

Molecular characterization of the first satellite DNA with CENP‐B and CDEIII motifs in the bat Pipistrellus kuhli

Fantaccione¹,

Pontecorvo²,

Zampella³

2005

FEBS Letters

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The centromere is an essential structure in the chromosomes of all eukariotes and is central to the mechanism that ensures proper segregation during mitosis and meiosis. The comparison of DNA sequence motifs, organization and kinetocore components from yeast to man is beginning to indicate that, although centromeres are highly variable DNA elements, a conserved pattern of sequence arrangement and function is emerging. We have identified and characterized the first satellite DNA (P.k.SAT) from microbat species Pipistrellus kuhli. The presence of mammalian CENP-B box and yeast CDEIII box could indicate the participation of P.k.SAT in centromere organization.

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Molecular characterization of a centromeric satellite DNA in the hemiclonal hybrid frogRana esculenta and its parental species

Cited by 51 publications

References 32 publications

Isolation and expression ofRlYB2, a germ cell‐specificY‐boxgene inRana

Isolation and expression ofRlYB2, a germ cell‐specificY‐boxgene inRana

Gametogenesis of intergroup hybrids of hemiclonal frogs

Molecular characterization of the first satellite DNA with CENP‐B and CDEIII motifs in the bat Pipistrellus kuhli

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