Molecular characterization of a putative gammapartitivirus in the phytopathogenic fungus Nigrospora oryzae

Yu, Jin Xiu; Zhu, Jun; Wang, Ying; Zhang, Chao Jun; Zhong, Jie; Zhu, Hong; Gao, Bingli; Zhou, Qian

doi:10.1007/s00705-017-3671-z

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…This fungus always causes leaf spot and rot diseases, which are responsible for yield losses of many economically important crops, including rice ( Oryza sativa ), wheat ( Triticum aestivum ), maize ( Zea mays ), sorghum ( Sorghum bicolor ), cotton ( Gossypium hirsutum ), aloe ( Aloe vera ), and kiwifruit ( Actinidia Chinensis ) [19,20,21,22]. At present, a few mycoviruses have been characterized from N. oryzae , including a novel partitiviruses [23] and a fusarivirus [24].…”

Section: Introductionmentioning

confidence: 99%

A Victorivirus and Two Novel Mitoviruses Co-Infected the Plant Pathogen Nigrospora oryzae

Liu

et al. 2019

Viruses

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Three dsRNAs, in sizes of approximately 2.5–5 kbp, were detected in the plant pathogenic fungus Nigrospora oryzae strain CS-7.5-4. Genomic analysis showed that the 5.0 kb dsRNA was a victorivirus named as Nigrospora oryzae victorivirus 2 (NoRV2). The genome of NoRV2 was 5166 bp in length containing two overlapping open reading frames (ORFs), ORF1 and ORF2. ORF1 was deduced to encode a coat protein (CP) showing homology to the CPs of viruses belonging to the Totiviridae family. The stop codon of ORF1 and the start codon of ORF2 were overlapped by the tetranucleotide sequence AUGA. ORF2 was predicted to encode an RNA-dependent RNA polymerase (RdRp), which was highly similar to the RdRps of victoriviruses. Virus-like particle examination demonstrated that the genome of NoRV2 was solely encapsidated by viral particles with a diameter of approximately 35 nm. The other two dsRNAs that were less than 3.0 kb were predicted to be the genomes of two mitoviruses, named as Nigrospora oryzae mitovirus 1 (NoMV1) and Nigrospora oryzae mitovirus 2 (NoMV2). Both NoMV1 and NoMV2 were A-U rich and with lengths of 2865 and 2507 bp, respectively. Mitochondrial codon usage inferred that each of the two mitoviruses contains a major large ORF encoding a mitoviral RdRp. Horizontal transfer experiments showed that the NoMV1 and NoMV2 could be cotransmitted horizontally via hyphal contact to other virus-free N. oryzae strains and causes phenotypic change to the recipient, such as an increase in growth rate. This is the first report of mitoviruses in N. oryzae.

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Section: Introductionmentioning

confidence: 99%

A Victorivirus and Two Novel Mitoviruses Co-Infected the Plant Pathogen Nigrospora oryzae

Liu

et al. 2019

Viruses

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“…The 3 UTR sequence was folded into two hairpins with perfect base paring in their stems (Figure 2D). Such hairpin structures are common in the UTRs of other dsRNA mycoviruses [32,[43][44][45]66,67] and are presumed to play an important role in viral replication and assembly [32,46,47]. The IGR sequence between ORF1 and ORF2 is 58 nt long and forms two hairpins that are separated by a 9 nt stretch (Figure 2D).…”

Section: Sequence Analysis Of the Mycovirus Infecting The 9-1 Isolate...mentioning

confidence: 99%

“…Recently, a genome draft of N. oryzae was released [43] which will definitely accelerate basic research on this pathogenic fungus. So far, several mycoviruses have been found in N. oryzae, including N. oryzae victorivirus 1 [44], N. oryzae victorivirus 2 [45], N. oryzae mitovirus 1/2 [45], N. oryzae fusarivirus 1 [46], N. oryzae partitivirus 1 [47], and N. oryzae nonsegmented RNA virus 1 (NoNRV1) [48], which would make N. oryzae an ideal microorganism for the study of mycovirus-host interactions. NoNRV1 is an unclassified virus and has a dsRNA genome of 2857 bp encoding two separated open reading frames (ORFs).…”

Section: Introductionmentioning

confidence: 99%

Infection of Nigrospora nonsegmented RNA Virus 1 Has Important Biological Impacts on a Fungal Host

Wang

Lai

et al. 2022

Viruses

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Nigrospora nonsegmented RNA virus 1 (NoNRV1) has been reported previously in the fungus Nigrospora oryzae, but its biological effects on its host are unknown. In this work, we isolated a strain 9-1 of N. oryzae from a chrysanthemum leaf and identified NoNRV1 infection in the isolated strain. The genome sequence of NoNRV1 identified here is highly homologous to that of the isolate HN-21 of NoNRV1 previously reported; thus, we tentatively designated the newly identified NoNRV1 as NoNRV1-ZJ. Drug treatment with Ribavirin successfully removed NoNRV1-ZJ from the strain 9-1, which provided us with an ideal control to determine the biological impacts of NoNRV1 infection on host fungi. By comparing the virus-carrying (9-1) and virus-cured (9-1C) strains, our results indicated that infection with NoNRV1 promoted the pigmentation of the host cells, while it had no discernable effects on host growth on potato dextrose agar plates when subjected to osmotic or oxidative stress. Interestingly, we observed inhibitory impacts of virus infection on the thermotolerance of N. oryzae and the pathogenicity of the host fungus in cotton leaves. Collectively, our work provides clear evidence of the biological relevance of NoNRV1 infection in N. oryzae, including pigmentation, hypovirulence, and thermotolerance.

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Molecular characterization of a new gammapartitivirus isolated from the citrus-pathogenic fungus Penicillium digitatum

et al. 2018

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To date, partitiviruses, including gammapartitiviruses, have been extensively studied in various fungal hosts but have not been reported in Penicillium digitatum (also called green mold, the pathogenic fungus infecting citrus). In the present work, we isolated and molecularly characterized a double-stranded RNA (dsRNA) partitivirus from citrus green mold, which we have named "Penicillium digitatum gammapartitivirus 1" (PdGV1). The bisegmented genome of PdGV1 contains two dsRNA segments (dsRNA1 and dsRNA2) with a length of 1795 bp and 1622 bp, respectively. Each of the two genomic dsRNAs contains a single open reading frame encoding a putative RNA-dependent RNA polymerase (RdRp) and a coat protein (CP), respectively. Phylogenetic analysis based on RdRp and CP sequences showed that PdGV1 clustered with mycoviruses belonging to the genus Gammapartitivirus, family Partitiviridae, e.g., Penicillium stoloniferum virus S. The 5'- and 3'-untranslated regions (UTRs) of the PdGV1 genomic dsRNAs both contained unique conserved RNA motifs that have never been found in any other partitivirus. This is the first report of a new gammapartitivirus that infects the citrus-pathogenic fungus P. digitatum.

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Molecular characterization of a putative gammapartitivirus in the phytopathogenic fungus Nigrospora oryzae

Cited by 5 publications

References 23 publications

A Victorivirus and Two Novel Mitoviruses Co-Infected the Plant Pathogen Nigrospora oryzae

A Victorivirus and Two Novel Mitoviruses Co-Infected the Plant Pathogen Nigrospora oryzae

Infection of Nigrospora nonsegmented RNA Virus 1 Has Important Biological Impacts on a Fungal Host

Molecular characterization of a new gammapartitivirus isolated from the citrus-pathogenic fungus Penicillium digitatum

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