Molecular characterization of novel mitochondrial peroxiredoxins from the Antarctic emerald rockcod and their gene expression in response to environmental warming

Tolomeo, Anna Maria; Carraro, Andrea; Bakiu, Rigers; Toppo, Stefano; Garofalo, Filippo; Pellegrino, Daniela; Gerdol, Marco; Ferro, Diana; Place, Sean P.; Santovito, Gianfranco

doi:10.1016/j.cbpc.2019.108580

Cited by 31 publications

(28 citation statements)

References 55 publications

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“…From this viewpoint, it is interesting that SOD1s of notothenioid fish have a molecular structure characterized by some portions with a highly negative surface charge, which is particularly abundant in the T. bernacchii enzyme. This electrostatic surface is present in other antioxidant enzymes from Antarctic fish, such as peroxiredoxins [73,74], and could constitute a possible molecular adaptation to low temperatures. As a partial confirmation of this hypothesis, the SOD1 from the tropical perciform S. partitus has a lower total negative charge.…”

Section: Discussionmentioning

confidence: 99%

Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature

et al. 2020

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In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Chionodraco hamatus, an Antarctic teleost widely distributed in many areas of the Ross Sea that plays a pivotal role in the Antarctic food chain. The primary sequence was obtained using biochemical and molecular biology approaches and compared with Cu,Zn SODs from other organisms. Multiple sequence alignment using the amino acid sequence revealed that Cu,Zn SOD showed considerable sequence similarity with its orthologues from various vertebrate species, but also some specific substitutions directly linked to cold adaptation. Phylogenetic analyses presented the monophyletic status of Antartic Teleostei among the Perciformes, confirming the erratic differentiation of these proteins and concurring with the theory of the “unclock-like” behavior of Cu,Zn SOD evolution. Expression of C. hamatus Cu,Zn SOD at both the mRNA and protein levels were analyzed in various tissues, highlighting the regulation of gene expression related to environmental stress conditions and also animal physiology. The data presented are the first on the antioxidant enzymes of a fish belonging to the Channichthyidae family and represent an important starting point in understanding the antioxidant systems of these organisms that are subject to constant risk of oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature

et al. 2020

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“…What does not emerge from protein–protein interaction network analysis is the correlation between GPxs and peroxiredoxins (Prdxs). Although the biological role of Prdxs is comparable to that of GPxs as scavenger against peroxides [ 41 , 42 ], only recently these proteins have been described in T. thermophila [ 43 ]. Therefore, more information is needed to better recognize the physiological role of these proteins.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization, Protein–Protein Interaction Network, and Evolution of Four Glutathione Peroxidases from Tetrahymena thermophila

et al. 2020

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Glutathione peroxidases (GPxs) form a broad family of antioxidant proteins essential for maintaining redox homeostasis in eukaryotic cells. In this study, we used an integrative approach that combines bioinformatics, molecular biology, and biochemistry to investigate the role of GPxs in reactive oxygen species detoxification in the unicellular eukaryotic model organism Tetrahymena thermophila. Both phylogenetic and mechanistic empirical model analyses provided indications about the evolutionary relationships among the GPXs of Tetrahymena and the orthologous enzymes of phylogenetically related species. In-silico gene characterization and text mining were used to predict the functional relationships between GPxs and other physiologically-relevant processes. The GPx genes contain conserved transcriptional regulatory elements in the promoter region, which suggest that transcription is under tight control of specialized signaling pathways. The bioinformatic findings were next experimentally validated by studying the time course of gene transcription and enzymatic activity after copper (Cu) exposure. Results emphasize the role of GPxs in the detoxification pathways that, by complex regulation of GPx gene expression, enable Tethraymena to survive in high Cu concentrations and the associated redox environment.

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“…PFOA is found at low levels in the serum of most people living in the United States, with higher levels observed in occupationally exposed workers [16,91]. PFOA was reported to induce liver, testes, and pancreatic tumors in male rats over a 2-year period [92].…”

Section: First Case: the Usamentioning

confidence: 99%

“…However, when using the animal data, the relevance between human and animal response to adverse health effects should be clarified. Given the apparent between-species differences in pharmacokinetics and tissue distribution of PFAS, as well as functional and structural differences in PPARs [16,80,81,286], caution must be taken when extrapolating data regarding PFAS from animal studies to humans. Steenland et al [287] suggested that higher serum PFOS was associated with higher levels of TC, LDL, and TG.…”

Section: Cholesterolmentioning

confidence: 99%

“…All living organisms respond to a high presence of ROS by increasing the expression of antioxidant enzymes and many other molecules that are elements of the defense and repair toward oxidative damage [10]. Enzymatic antioxidants include proteins such as superoxide dismutase (SOD) [11,12], catalase (CAT) [13], glutathione peroxidase (GPx) [14], peroxiredoxins (Prdx) [15,16], and methionine sulfoxide reductases (Msr) [17]. These enzymes represent a first line against ROS, but do not guarantee complete coverage from the risk of oxidative stress because some compounds, generated by their reactions, are still reactive and potentially cytotoxic, such as the hydrogen peroxide (H 2 O 2 ) produced by SOD.…”

Section: Introductionmentioning

confidence: 99%

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PFAS Environmental Pollution and Antioxidant Responses: An Overview of the Impact on Human Field

Bonato

Corrà

Belli̇o

et al. 2020

IJERPH

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Due to their unique properties, perfluorinated substances (PFAS) are widely used in multiple industrial and commercial applications, but they are toxic for animals, humans included. This review presents some available data on the PFAS environmental distribution in the world, and in particular in Europe and in the Veneto region of Italy, where it has become a serious problem for human health. The consumption of contaminated food and drinking water is considered one of the major source of exposure for humans. Worldwide epidemiological studies report the negative effects that PFAS have on human health, due to environmental pollution, including infertility, steroid hormone perturbation, thyroid, liver and kidney disorders, and metabolic disfunctions. In vitro and in vivo researches correlated PFAS exposure to oxidative stress effects (in mammals as well as in other vertebrates of human interest), produced by a PFAS-induced increase of reactive oxygen species formation. The cellular antioxidant defense system is activated by PFAS, but it is only partially able to avoid the oxidative damage to biomolecules.

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Molecular characterization of novel mitochondrial peroxiredoxins from the Antarctic emerald rockcod and their gene expression in response to environmental warming

Cited by 31 publications

References 55 publications

Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature

Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature

Molecular Characterization, Protein–Protein Interaction Network, and Evolution of Four Glutathione Peroxidases from Tetrahymena thermophila

PFAS Environmental Pollution and Antioxidant Responses: An Overview of the Impact on Human Field

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