Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis

Khamrin, Pattara; Maneekarn, Niwat; Peerakome, Supatra; Tonusin, Supin; Phan, Tung Gia; Okitsu, Shoko; Ushijima, Hiroshi

doi:10.1002/jmv.20840

Cited by 39 publications

(45 citation statements)

References 43 publications

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“…Additionally, the detections of multiple combinations of G3 with several P genotypes such as G3P[3] [Khamrin et al, 2006a], G3P[9] [Khamrin et al, 2007b], G3P[10] (in the present study), and G3P[19] [Maneekarn et al, 2006] in the same geographical area (Chiang Mai City) suggests that reassortment events among rotaviruses circulating in that area are taken place frequently. Furthermore, analysis of the NSP5/6 sequence of CMH079/05 revealed that it was most closely related with that of T152, a G12P[9] human rotavirus strain isolated in 1998 from an 11-month-old child admitted to hospital in Bangkok, Thailand [Pongsuwanna et al, 2002].…”

Section: Discussionsupporting

confidence: 50%

“…Recently, two other isolates (CMH120/04 and CMH134/04) of unusual human rotavirus G3P [9] genotype were detected during an epidemiological survey of human rotavirus infection in Chiang Mai, Thailand in 2004. Genetic analyses of VP4, VP6, VP7, and NSP4 genes of these G3P[9] strains revealed a close genetic relationship with rotavirus strains isolated previously from felines and humans [Khamrin et al, 2007b].…”

Section: Discussionmentioning

confidence: 99%

“…Those epidemiological studies have demonstrated that rotavirus genotypes G1, G2, G3, G4, and G9 in combination with P[8] and P [4] genotypes are the most common combinations associated with human rotavirus infection globally [Gentsch et al, 2005;Santos and Hoshino, 2005] [Uchida et al, 2006;Khamrin et al, 2006aKhamrin et al, , 2007bDuan et al, 2007;Ghosh et al, 2007;Steyer et al, 2007b]. In addition, the increasing data on the onset of animal-like rotavirus strains in the human population has demonstrated the importance of direct interspecies transmission of animal strains into humans and genetic reassortment between human and animal rotavirus strains.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of VP4, VP6, VP7, NSP4, and NSP5/6 genes identifies an unusual G3P[10] human rotavirus strain

Khamrin

Maneekarn

Peerakome

et al. 2008

Journal of Medical Virology

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An unusual strain of human rotavirus G3P [10] (CMH079/05) was detected in a stool sample of a 2-year-old child admitted to the hospital with severe diarrhea in Chiang Mai, Thailand. Analysis of the VP7 gene sequence revealed highest identities with unusual human rotavirus G3 strain CMH222 at 98.7% on the nucleotide and 99.6% on the amino acid levels. Phylogenetic analysis of the VP7 sequence confirmed that the CMH079/05 strain formed a cluster with G3 rotavirus reference strains and showed the closest lineage with the CMH222 strain. Analysis of partial VP4 gene of CMH079/05 revealed highest degree of sequence identities with P[10] rotavirus prototype strain 69M at nucleotide and amino acid levels of 92.9% and 94.6%, respectively. Phylogenetic analysis of the VP4 sequence revealed that CMH079/05 and 69M clustered closely together in a monophyletic branch separated from other rotavirus genotypes. To our knowledge, this is a novel G-P combination of G3 and P[10] genotypes. In addition, analyses of VP6, NSP4, and NSP5/6 genes revealed these uncommon genetic characteristics: (i) the VP6 gene differed from the four other known subgroups; (ii) the NSP4 gene was identified as NSP4 genetic group C, an uncommon group in humans; and (iii) the NSP5/6 gene was most closely related with T152, a G12P[9] rotavirus previously isolated in Thailand. The finding of uncommon G3P [10] rotavirus in this pediatric patient provided additional evidence of the genetic diversity of human group A rotaviruses in Chiang Mai, Thailand.

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Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of VP4, VP6, VP7, NSP4, and NSP5/6 genes identifies an unusual G3P[10] human rotavirus strain

Khamrin

Maneekarn

Peerakome

et al. 2008

Journal of Medical Virology

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“…In humans, G3 rotaviruses are usually associated with the P[8] VP4 genotype and, more rarely, with the P [9] genotype (10,15,21). Conversely, the G3P [9] combination is common in feline rotaviruses (11).…”

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confidence: 99%

“…Strain PAF96/94 was the only G3 rotavirus detected in 1994, while strains PAH136/96 and PAI58/96 were detected in 1996, along with a few G3P [8] viruses. The P [9] genotype is responsible for less than 2.5% of the rotavirus infections among humans worldwide, while it is common in feline rotaviruses (15). After their initial description in 1982 in Israel, G3P [9] strains have been detected in humans on more occasions, but only in a sporadic fashion.…”

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Rare AU-1-Like G3P[9] Human Rotaviruses with a Kun-Like NSP4 Gene Detected in Children with Diarrhea in Italy

et al. 2008

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Three G3P[9] rotaviruses, detected in children hospitalized with gastroenteritis in Palermo, Italy, were found to be genetically related to strains of either human or feline origin in the VP7, VP4, and VP6 genes. In contrast, in the NSP4 gene the viruses resembled G2P[4] human strains, suggesting a reassortment between AU-1-like and Kun-like strains.Group A rotaviruses are a major cause of acute gastroenteritis in humans and animals (14). The rotavirus genome is composed of 11 segments of double-stranded RNA (dsRNA) (4). The pattern of migration of the dsRNA by polyacrylamide gel electrophoresis (PAGE) allows the distinction of a long, short, or supershort electropherotype (e-type) (4). The viruses are classified as G and P types on the basis of the outer capsid proteins VP7 and VP4, respectively (4). Of the 15 G types and the 27 P types, 5 G types (types G1 to G4 and G9) and three P types (types P[4], P[6], and P[8]) appear to be common in human rotaviruses (21). On the basis of their reactivities to VP6-specific monoclonal antibodies, group A rotaviruses are classified into types SGI, SGII, SGI ϩ SGII, and SG-non I-non II (4). The majority of SGI and SGII animal rotavirus strains and SGII human rotavirus strains display a long e-type of migration of the 11 dsRNA gene segments, while almost all SGI human rotaviruses possess a short e-type (14). The nonstructural protein NSP4 is able to induce diarrhea in experimental rodents through a Ca 2ϩ -dependent signaling pathway (18). Passively acquired antibodies to NSP4 appear to prevent watery diarrhea in mice (2), and this sets NSP4 as a potential vaccine target. Six NSP4 genotypes (genotypes A to F) have been established in group A rotaviruses. In humans, NSP4 genotypes A (Kun-like) and B (Wa-like) are common. Genotype C (AU-1-like) is common in feline and canine strains but is infrequent in human rotaviruses (3).The G3 VP7 specificity has been identified in rotavirus strains from almost all susceptible animal species (4). In humans, G3 rotaviruses are usually associated with the P[8] VP4 genotype and, more rarely, with the P[9] genotype (10,15,21). Conversely, the G3P[9] combination is common in feline rotaviruses (11). Interspecies infections between animals and humans can be revealed by the detection of strains with unexpected antigenic and genetic features. The first human G3P [9] rotavirus strain, strain AU-1, was isolated in Israel in 1982. The strain displayed animal-like features (a long e-type in conjunction with SGI specificity) and was shown by RNA-RNA hybridization to be closely related to feline rotaviruses (19).Uninterrupted surveillance activity for rotaviruses has been conducted in Palermo, Italy, since the mid-1980s. Between 1985 and 2005, 1,547 rotavirus-positive samples were collected and characterized either antigenically or genetically to predict the VP7, VP4, and VP6 specificities and the genome pattern. Three strains (strains PAF96/94, PAH136/96, and PAI58/96) displayed animal strain-like features, since they possessed a long e-type by PAGE an...

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Changing pattern of rotavirus G genotype distribution in Chiang Mai, Thailand from 2002 to 2004: Decline of G9 and reemergence of G1 and G2

Khamrin

Peerakome

Tonusin³

et al. 2007

Journal of Medical Virology

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Group A rotaviruses are the most common cause of acute viral diarrhea in humans and animals throughout the world. Previous surveillance studies of group A rotaviruses in Thailand indicated that the dominant types of rotaviruses were changing from time to time. During 2000 and 2001, the G9 rotavirus emerged as the most prevalent genotype, with an exceptionally high frequency (91.6%) in Chiang Mai, Thailand. In the year 2002-2004, group A rotavirus was detected in 98 out of 263 (37.3%) fecal specimens collected from children hospitalized with diarrhea. Of these, 40 (40.8%) were G9P[8], 33 (33.7%) were G1P[8], 23 (23.5%) were G2P[4], and 2 (2.0%) were G3P[9]. The G9P[8] was found to be the most predominant strain in 2002, but the prevalence rate abruptly decreased during the period 2003-2004. In addition, G2P[4] reemerged in the epidemic season of 2003, whereas G1P[8] became the most predominant strain in the following year (2004). Phylogenetic analysis of the VP7 genes revealed that G1, G2, and G9 rotavirus strains clustered together with recently circulating strains, which were isolated from different regional settings in Thailand. In conclusion, the study demonstrated a decrease of incidence of G9P[8] and reemergence of G1P[8] and G2P[4] rotaviruses in Chiang Mai, Thailand during the period 2002-2004. These data imply that the distribution of group A rotavirus genotypes circulating in Chiang Mai, Thailand, changes over time.

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Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis

Cited by 39 publications

References 43 publications

Molecular characterization of VP4, VP6, VP7, NSP4, and NSP5/6 genes identifies an unusual G3P[10] human rotavirus strain

Molecular characterization of VP4, VP6, VP7, NSP4, and NSP5/6 genes identifies an unusual G3P[10] human rotavirus strain

Rare AU-1-Like G3P[9] Human Rotaviruses with a Kun-Like NSP4 Gene Detected in Children with Diarrhea in Italy

Changing pattern of rotavirus G genotype distribution in Chiang Mai, Thailand from 2002 to 2004: Decline of G9 and reemergence of G1 and G2

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