Molecular Characterization of Selected Local and Exotic Cattle Using RAPD Marker

Khatun, M. Mahfuza; Hossain, Kamal; Rahman, Suzanah Abdul

doi:10.5713/ajas.2011.11331

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…Five of twenty primers able to produce clearly visible bands were selected. The clearance of bands depends on the quality of DNA the primary sequence which has more than 60% of GC suitable for RAPD analysis (Khatun, Hossain, & Mahbubur Rahman, 2012). Bands readability is also important in genetic diversity analysis.…”

Section: Resultsmentioning

confidence: 99%

Study on Diversity of Sapodilla (Manilkara zapota) by Molecular Marker in the Special Region of Yogyakarta

2018

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The objective of this research was to determine the diversity and relationship of sapodilla accession derived from districts in DIY based on DNA profiles. From the screening of 20 total primers, five primers (OPA 20, OPB 5, OPB 6, OPB 8, and OPC 19) producing polymorphic bands in RAPD analysis were selected. Different-shaped samples of sapodilla randomly collected from Bantul, Gunungkidul, Kulonprogo, Sleman and Yogyakarta city were used in this research. The analysis of molecular data was performed by using NTSys pc-2.02 and GenAlex 6.1 program. The clustering indicated that the accessions tend to group by their locations. However, there was no corelation between DNA marker and the fruit shape. The first group comprised samples from Yogyakarta city (Keraton Yogyakarta), Bantul and Kulonprogo while the second group consisted of samples from Sleman and Gunungkidul with genetic similarity of 53% and 37% respectively. The genetic diversity was high (PLP = 98.85% of 87 loci and H=0.280±0.14) comprising the genetic diversity within population (53%) which was greater than genetic diversity between populations (47%). Having the highest genetic diversity, Gunungkidul should be potential for conservation and selection of sapodilla in DIY.

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Section: Resultsmentioning

confidence: 99%

Study on Diversity of Sapodilla (Manilkara zapota) by Molecular Marker in the Special Region of Yogyakarta

2018

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“…Polymorphism between individuals is caused by sequence differences in one or both primer binding sites and can be seen from the presence or absence of certain RAPD bands (Khatun at al., 2012). The RAPD method is simple but has low reproducibility, another disadvantage is that it requires a wide variety of primers such as for forensic testing.…”

Section: Current Condition (State Of the Art) Research On Nutrigenomi...mentioning

confidence: 99%

Omics Technology for Genetic Selection Towards Feed Efficiency Traits of Indigenous Cattle in Indonesia: A Review

Soetanto,

Fatchiyah

2023

JNT

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Indigenous cattle in Indonesia seldom to reach their genetic potentials due to an interaction impact of harsh tropical climate and low-quality feed. Some indigenous breeds of cattle in Indonesia, such as Bali, Madura and Crossbred Ongole have demonstrated their successful adaptation to survive in the harsh environment, but their ultimate performances are still considered less than their genetic potentials when reared under comfortable environment like in the sub-tropical climate. This is primarily due to inefficient fermentation of feed originating from inadequate supply of essential nutrients required for optimum growth of microbes in the forestomach. A rapid advancement of omics technology has been the driver for ruminant nutritionists to use such a technology to study the interrelationship between nutrients and particular genes associated with the biochemical pathways of nutrients at molecular levels. This review highlights the current issues and challenges on the use of omics technologies to improve the efficiency of feed utilization by cattle consuming low-quality diets and its potential to be used in the genetic selection program.

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“…Several molecular markers like RAPD, RFLP and microsatellites are being used for molecular characterization of animal genetic resources [10][11][12]. In the last decade, mitochondrial DNA (mtDNA) has emerged as a very powerful and reliable marker in animal population and evolutionary biology [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Origin, genetic diversity and evolution of Andaman local duck, a native duck germplasm of an insular region of India

Sawhney

Bhattacharya

et al. 2021

PLoS ONE

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Domestic ducks are of paramount importance as a cheap source of protein in rural India. Andaman local duck (ALD) is an indigenous avian genetic resource of Andaman and Nicobar islands (ANI) and is mainly distributed in Middle and Northern parts of these islands. Negligence has brought this breed on the edge of extinction necessitating immediate conservation efforts. Here, we report the genetic diversity, population structure and matrilineal genetic root of ALD. Partial mtDNA D-loop sequences were analyzed in 71 ALD samples and analysis revealed 19 polymorphic sites and 13 haplotypes. Estimated haplotype (Hd ± SD) and nucleotide diversity (π ± SD) were 0.881 ± 0.017 and 0.00897 ± 0.00078 respectively. The high genetic diversity of ALD indicates introgression of genetic material from other local duck breeds. In addition, it can be postulated that ALD bearing high genetic diversity has strong ability to adapt to environmental changes and can withstand impending climate change. Phylogenetic and network analysis indicate that ALD falls under Eurasian clade of mallard and ALD forms three clusters; one cluster is phylogenetically close to Southeast Asian countries, one close to Southern part of mainland India and the third one forms an independent cluster. Therefore, ALD might have migrated either from Southeast Asian countries which enjoy a close cultural bondage with ANI from time immemorial or from Southern part of India. The independent cluster may have evolved locally in these islands and natural selection pressure imposed by environmental conditions might be the driving force for evaluation of these duck haplotypes; which mimics Darwin’s theory of natural selection. The results of the study will be beneficial for formulating future breeding programme and conservation strategy towards sustainable development of the duck breed.

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Molecular Characterization of Selected Local and Exotic Cattle Using RAPD Marker

Cited by 5 publications

References 18 publications

Study on Diversity of Sapodilla (Manilkara zapota) by Molecular Marker in the Special Region of Yogyakarta

Study on Diversity of Sapodilla (Manilkara zapota) by Molecular Marker in the Special Region of Yogyakarta

Omics Technology for Genetic Selection Towards Feed Efficiency Traits of Indigenous Cattle in Indonesia: A Review

Origin, genetic diversity and evolution of Andaman local duck, a native duck germplasm of an insular region of India

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