Molecular Characterization of the Putative Transcription Factor SebA Involved in Virulence in Aspergillus fumigatus

Dinamarco, Taísa Magnani; Almeida, Ricardo Sérgio; Castro, Patrícia Alves de; Brown, Neil Andrew; Reis, Thaila Fernanda dos; Ramalho, Leandra Náira Zambelli; Savoldi, Marcela; Goldman, Maria Helena de Souza; Goldman, Gustavo Henrique

doi:10.1128/ec.00016-12

Cited by 41 publications

(56 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In many systems, the transcriptional machinery that regulates gene expression in response to ROS is well characterized (12,13,(47)(48)(49)(50)(51)(52). In the pathogenic protist E. histolytica, increased virulence has been linked to the parasite's ability to survive exposure to ROS (21), and the transcriptional response to hydrogen peroxide stress has been characterized (2).…”

Section: Discussionmentioning

confidence: 99%

Regulation of H2O2 Stress-responsive Genes through a Novel Transcription Factor in the Protozoan Pathogen Entamoeba histolytica

Pearson

Morf

Singh

2013

Journal of Biological Chemistry

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Regulation of H2O2 Stress-responsive Genes through a Novel Transcription Factor in the Protozoan Pathogen Entamoeba histolytica

Pearson

Morf

Singh

2013

Journal of Biological Chemistry

View full text Add to dashboard Cite

“…Moreover, SEB1 is involved in but not essential for glycerol dehydrogenase (gld1) gene expression and glycerol accumulation in T. atroviride during osmotic stress (1024). In A. fumigatus, SEB1 is involved in the response to oxidative stress and virulence, and the absence of seb1 results in the deregulation of Afatf1 and Afyap1, which have also been implicated in the response to stress (1025).…”

Section: C2h2 Zinc Finger Transcription Factorsmentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

165

195

View full text Add to dashboard Cite

SUMMARYThe genusTrichodermacontains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, comprehensive overview of the genomic content of these species for “hot topic” research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism inT. reesei,T. atroviride, andT. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of eachTrichodermaspecies discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved inN-linked glycosylation was detected, as were indications for the ability ofTrichodermaspp. to generate hybrid galactose-containingN-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique toTrichoderma, and these warrant further investigation. We found interesting expansions in theTrichodermagenus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique toT. atrovirideis the duplication of the alternative sulfur amino acid synthesis pathway.

show abstract

“…The murine model of pulmonary aspergillosis was performed according to the method of Dinamarco et al (44). Outbreed female mice (BALB/c strain; body weight, 20 to 22 g) were housed in vented cages containing 5 animals.…”

Section: Methodsmentioning

confidence: 99%

“…The AMs were counted in a hemocytometer. The phagocytic assay was performed according to the methods of Dinamarco et al and Mech et al (44,45), with some changes. Briefly, in a 24-well plate containing one 15-mm-diameter coverslip per well, about 2 ϫ 10 4 macrophages were incubated with 1 ml of RPMI-FBS at 37°C with 5% CO 2 for 1 h. Afterward, the wells were washed with 1 ml of assay medium to remove nonadherent cells.…”

Section: Methodsmentioning

confidence: 99%

“…After 10 min at 25°C, samples were removed from the plate and serially diluted in sterile saline. The dilutions were plated onto A. fumigatus complete medium (44) and incubated at 37°C for 2 days. Conidial killing was calculated by comparing CFU numbers from samples incubated with macrophages to CFU numbers from those incubated without macrophages.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The Aspergillus fumigatus sitA Phosphatase Homologue Is Important for Adhesion, Cell Wall Integrity, Biofilm Formation, and Virulence

et al. 2015

Self Cite

View full text Add to dashboard Cite

h Aspergillus fumigatus is an opportunistic pathogenic fungus able to infect immunocompromised patients, eventually causing disseminated infections that are difficult to control and lead to high mortality rates. It is important to understand how the signaling pathways that regulate these factors involved in virulence are orchestrated. Protein phosphatases are central to numerous signal transduction pathways. Here, we characterize the A. fumigatus protein phosphatase 2A SitA, the Saccharomyces cerevisiae Sit4p homologue. The sitA gene is not an essential gene, and we were able to construct an A. fumigatus null mutant. The ⌬sitA strain had decreased MpkA phosphorylation levels, was more sensitive to cell wall-damaging agents, had increased ␤-(1,3)-glucan and chitin, was impaired in biofilm formation, and had decreased protein kinase C activity. The ⌬sitA strain is more sensitive to several metals and ions, such as MnCl 2 , CaCl 2 , and LiCl, but it is more resistant to ZnSO 4 . The ⌬sitA strain was avirulent in a murine model of invasive pulmonary aspergillosis and induces an augmented tumor necrosis factor alpha (TNF-␣) response in mouse macrophages. These results stress the importance of A. fumigatus SitA as a possible modulator of PkcA/MpkA activity and its involvement in the cell wall integrity pathway.

show abstract

Molecular Characterization of the Putative Transcription Factor SebA Involved in Virulence in Aspergillus fumigatus

Cited by 41 publications

References 65 publications

Regulation of H2O2 Stress-responsive Genes through a Novel Transcription Factor in the Protozoan Pathogen Entamoeba histolytica

Regulation of H2O2 Stress-responsive Genes through a Novel Transcription Factor in the Protozoan Pathogen Entamoeba histolytica

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

The Aspergillus fumigatus sitA Phosphatase Homologue Is Important for Adhesion, Cell Wall Integrity, Biofilm Formation, and Virulence

Contact Info

Product

Resources

About