Molecular Characterization of the Thaumatin-like Protein PR-NP24 in Tomato Fruits

An, Mengnan; Tong, Zhipeng; Ding, Chengsong; Wang, Zehao; Sun, Hui; Xia, Zihao; Qi, Mingfang; Wu, Yuanhua; Liang, Yue

doi:10.1021/acs.jafc.9b05256

Cited by 13 publications

(8 citation statements)

References 49 publications

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“…A number of research studies have shown that TLPs are localized in the extracellular space, enhancing plant tolerance to various stresses [20,44]. TLP gene expression can be regulated by a variety of signals, such as SA, MeJA, abscisic acid, injury, UV, osmotic stress, and invasion by bacteria, fungi, viruses, and other organisms [45]. In the GbTLP promoter regions, we found a number of stress-responsive cis-elements, such as ARE, MBS, LTR, WUN-motif, and TC-rich repeats, which are responsive to biotic and abiotic stresses (Figure 5).…”

Section: The Putative Regulatory Mechanisms Of Gbtlp Gene Expressionmentioning

confidence: 99%

Genome-Wide Identification of the Thaumatin-like Protein Family Genes in Gossypium barbadense and Analysis of Their Responses to Verticillium dahliae Infection

Zhang

Chen

Sang

et al. 2021

Plants

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(1) Background: Plants respond to pathogen challenge by activating a defense system involving pathogenesis-related (PR) proteins. The PR-5 family includes thaumatin, thaumatin-like proteins (TLPs), and other related proteins. TLPs play an important role in response to biotic and abiotic stresses. Many TLP-encoding genes have been identified and functionally characterized in the model plant species. (2) Results: We identified a total of 90 TLP genes in the G. barbadense genome. They were phylogenetically classified into 10 subfamilies and distributed across 19 chromosomes and nine scaffolds. The genes were characterized by examining their exon–intron structures, promoter cis-elements, conserved domains, synteny and collinearity, gene family evolution, and gene duplications. Several TLP genes were predicted to be targets of miRNAs. Investigation of expression changes of 21 GbTLPs in a G. barbadense cultivar (Hai7124) resistance to Verticillium dahliae revealed 13 GbTLPs being upregulated in response to V. dahliae infection, suggesting a potential role of these GbTLP genes in disease response. (3) Conclusions: The results of this study allow insight into the GbTLP gene family, identify GbTLP genes responsive to V. dahliae infection, and provide candidate genes for future studies of their roles in disease resistance.

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Section: The Putative Regulatory Mechanisms Of Gbtlp Gene Expressionmentioning

confidence: 99%

Genome-Wide Identification of the Thaumatin-like Protein Family Genes in Gossypium barbadense and Analysis of Their Responses to Verticillium dahliae Infection

Zhang

Chen

Sang

et al. 2021

Plants

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“…The pathogenic fungi, S. sclerotiorum and B. cinerea [29], were preserved in College of Plant Protection, Shenyang Agricultural University, China [29,30]. Mycelia cultured on PDA (potato dextrose agar, 20 g agar powder, 20 g D-glucose, 200 g potato) at 25 °C were used in subsequent experiments of mycelial growth and inoculation experiments.…”

Section: Pathogenic Fungi and Plantsmentioning

confidence: 99%

ε-poly-L-lysine Affects the Vegetative Growth, Pathogenicity and Expression Regulation of Necrotrophic Pathogen Sclerotinia sclerotiorum and Botrytis cinerea

Zhou

Huang

et al. 2021

JoF

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Microbial secondary metabolites produced by Streptomyces are applied to control plant diseases. The metabolite, ε-poly-L-lysine (ε-PL), is a non-toxic food preservative, but the potential application of this compound as a microbial fungicide in agriculture is rarely reported. In this study, the effect and mode of action of ε-PL on two necrotrophic pathogenic fungi, Sclerotinia sclerotiorum and Botrytis cinerea, were investigated. The results showed that ε-PL effectively inhibited the mycelial growth of S. sclerotiorum and B. cinerea with EC50 values of 283 μg/mL and 281 μg/mL, respectively. In addition, ε-PL at the dose of 150 and 300 μg/mL reduced S. sclerotiorum sclerotia formation. The results of the RNA-seq and RT-qPCR validation indicated that ε-PL significantly regulated the gene expression of critical differential expressed genes (DEGs) involved in fungal growth, metabolism, pathogenicity, and induced an increase in the expression of the fungal stress responses and the detoxification genes. These results provided new insights for understanding the modes of action of ε-PL on S. sclerotiorum and B. cinerea and improved the sustainable management of these plant diseases.

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“…[ 4 , 5 , 6 ]. TLPs could also be activated by bacterial pathogens, abiotic stresses (such as wounding, drought, osmotic stress, low temperature, high salinity, and UV radiation), and plant hormones [ 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Thaumatin-like Protein (TLP) Genes in Garlic (Allium sativum L.): Genome-Wide Identification, Characterization, and Expression in Response to Fusarium proliferatum Infection

Anisimova

Кочиева

Щенникова

et al. 2022

Plants

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Plant antifungal proteins include the pathogenesis-related (PR)-5 family of fungi- and other stress-responsive thaumatin-like proteins (TLPs). However, the information on the TLPs of garlic (Allium sativum L.), which is often infected with soil Fusarium fungi, is very limited. In the present study, we identified 32 TLP homologs in the A. sativum cv. Ershuizao genome, which may function in the defense against Fusarium attack. The promoters of A. sativumTLP (AsTLP) genes contained cis-acting elements associated with hormone signaling and response to various types of stress, including those caused by fungal pathogens and their elicitors. The expression of AsTLP genes in Fusarium-resistant and -susceptible garlic cultivars was differently regulated by F. proliferatum infection. Thus, in the roots the mRNA levels of AsTLP7–9 and 21 genes were increased in resistant and decreased in susceptible A. sativum cultivars, suggesting the involvement of these genes in the garlic response to F. proliferatum attack. Our results provide insights into the role of TLPs in garlic and may be useful for breeding programs to increase the resistance of Allium crops to Fusarium infections.

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Molecular Characterization of the Thaumatin-like Protein PR-NP24 in Tomato Fruits

Cited by 13 publications

References 49 publications

Genome-Wide Identification of the Thaumatin-like Protein Family Genes in Gossypium barbadense and Analysis of Their Responses to Verticillium dahliae Infection

Genome-Wide Identification of the Thaumatin-like Protein Family Genes in Gossypium barbadense and Analysis of Their Responses to Verticillium dahliae Infection

ε-poly-L-lysine Affects the Vegetative Growth, Pathogenicity and Expression Regulation of Necrotrophic Pathogen Sclerotinia sclerotiorum and Botrytis cinerea

Thaumatin-like Protein (TLP) Genes in Garlic (Allium sativum L.): Genome-Wide Identification, Characterization, and Expression in Response to Fusarium proliferatum Infection

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