Molecular characterization, polymorphism and association of Keratin-Associated Protein 13.1 (<i>KAP13.1</i>) gene in Changthangi goats

Shanaz, Syed; Ganai, T. A. S.; Ahmad, Sheikh Firdous; Khan, Azmat Aalam; Shabir, Nadeem; Shah, Ruksana; Shabir, Mir; Ganai, Nazir Ahmad

doi:10.1080/09291016.2018.1562122

Cited by 4 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…KAP22-1 gene variation in sheep affects wool curvature [28]. A negative correlation between KAP13-1 gene variation and fiber traits has been found in Changtang goat populations [29]. Variation in the KAP6-1 gene in Egyptian Barki sheep affects wool length, diameter and yield [30].…”

Section: Discussionmentioning

confidence: 99%

Identification of the Keratin-Associated Protein 22-2 Gene in the Capra hircus and Association of Its Variation with Cashmere Traits

Chen,

Cao,

Zhao

et al. 2023

Animals

View full text Add to dashboard Cite

The Cashmere goat is an excellent local goat breed in Gansu Province of China, and it is expected to improve cashmere production and cashmere quality through selection and breeding to enhance its commercial value. Keratin-associated proteins (KAPs) play an important role in maintaining wool structure. The gene encoding the keratin-associated protein 22-2 (KAP22-2) gene has been identified in selected species other than goats, such as humans, mice, and sheep. In this study, the sequence of the sheep KAP22-2 gene (KRTAP22-2) was aligned into the goat genome, and the sequence with the highest homology was assumed to be the goat KRTAP22-2 sequence and used to design primers to amplify the goat gene sequence. A total of 356 Longdong Cashmere goats (Gansu Province, China) were used for screening of genetic variants. Four specific bands were detected by polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) analysis, and they formed a total of six band types individually or in combination. Four alleles were identified by DNA sequencing of PCR amplification products. A total of four single nucleotide polymorphic sites (SNPs) were detected in the four sequenced KRTAP22-2 alleles. Two of them are in the 5’UTR region and the other two are in the coding region, and the variants in the coding region are all non-synonymous mutations. In addition, there was a 6 bp length variation in allele C. The gene was expressed in the cortical layer of primary and secondary hair follicles, the inner root sheath, as well as hair papillae and hair maternal cells in goats. The results of the correlation analysis between genotypes and cashmere traits showed that after excluding genotypes with a gene frequency of less than 5%, the mean fiber diameter (MFD) of cashmere was significantly higher in the AB genotype than in the AA and AC genotypes. That is, the KRTAP22-2 gene variants are associated with mean fiber diameter in cashmere. The above results suggest that the goat KRTAP22-2 variant can be utilized as a molecular marker candidate gene for cashmere traits.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of the Keratin-Associated Protein 22-2 Gene in the Capra hircus and Association of Its Variation with Cashmere Traits

Chen,

Cao,

Zhao

et al. 2023

Animals

View full text Add to dashboard Cite

show abstract

“…Fang et al ( 2010) used PCR-RFLP technology to study the correlation between the KRTAP13.1 gene and the cashmere traits of Jiangnan goats, and the results showed that there was no significant difference in cashmere fineness between the different genotypes (P > 0.05). Similarly, Shanaz et al (2020) used PCR-RFLP technology and found that the KRTAP13.1 gene polymorphism had no significant effect on the cashmere traits of Changthangi goats (P > 0.05). The above two loci are not present in our research results, which indicates the rich polymorphism of the KRTAP13.1 gene.…”

Section: Discussionmentioning

confidence: 99%

Correlation analysis of four KRTAP gene polymorphisms and cashmere fiber diameters in two cashmere goat breeds

Wua

Qin²,

et al. 2022

Can. J. Anim. Sci.

View full text Add to dashboard Cite

Fiber diameter, a quantitative trait, is controlled by minor effect polygenes. Keratin-associated proteins (KRTAPs) are an important part of hair, and their rich polymorphisms facilitate the mining of cashmere trait molecular markers. In this study, Jiangnan and Tibetan cashmere goats were taken as the research object; multiplex PCR and exome sequencing technology were used to identify the exon regional polymorphisms of cashmere goats KRTAP15-1, KRTAP13.1, KRTAP27-1, and KRTAP24-1. The effects of mutation sites on the fiber diameter of cashmere were analyzed by least square method. The results showed that there were 28 mutation sites in the four KRTAP genes in Jiangnan cashmere goats and Tibetan cashmere goat populations. Among them, the KRTAP13.1, KRTAP27-1, and KRTAP24-1 gene polymorphisms were found to be significantly related to the fiber diameter of Jiangnan cashmere goats. The exploration of molecular markers in this study will help to improve the fiber diameter of the down, while the identification of gene polymorphisms will provide original data for the utilization and protection of germplasm resources of cashmere goats.

show abstract

Characterization and functional analysis of Krtap11-1 during hair follicle development in Angora rabbits (Oryctolagus cuniculus)

et al. 2020

View full text Add to dashboard Cite

Molecular characterization, polymorphism and association of Keratin-Associated Protein 13.1 (KAP13.1) gene in Changthangi goats

Cited by 4 publications

References 7 publications

Identification of the Keratin-Associated Protein 22-2 Gene in the Capra hircus and Association of Its Variation with Cashmere Traits

Identification of the Keratin-Associated Protein 22-2 Gene in the Capra hircus and Association of Its Variation with Cashmere Traits

Correlation analysis of four KRTAP gene polymorphisms and cashmere fiber diameters in two cashmere goat breeds

Characterization and functional analysis of Krtap11-1 during hair follicle development in Angora rabbits (Oryctolagus cuniculus)

Contact Info

Product

Resources

About