Molecular cloning and characterization in eukaryotic expression systems of a sugarcane cysteine protease inhibitor gene involved in drought tolerance

Jangpromma, Nisachon; Saito, Akira; Araki, Toshimitsu; Jaisil, Prasit; Songsri, Patcharin; Daduang, Sakda; Kawaguchi, Yuya; Dhiravisit, Apisak; Thammasirirak, Sompong

doi:10.3906/bot-1310-46

Cited by 11 publications

(10 citation statements)

References 39 publications

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“…Leaf chlorophyll content can be determined by extraction with organic solvents including acetone [49] and methanol [50] and subsequent quantification using a spectrometer; however this method is expensive and time consuming [51]. A higher throughput non-destructive method is the SPAD chlorophyll meter which allows rapid and inexpensive assessment of leaf greenness [52].…”

Section: Chlorophyll Contentmentioning

confidence: 99%

“…A higher throughput non-destructive method is the SPAD chlorophyll meter which allows rapid and inexpensive assessment of leaf greenness [52]. SPAD measures leaf absorbance in the red (650 nm) and infrared (940 nm) regions [53] and gives readings that have been correlated with chlorophyll content under different moisture regimes in many crops [51].…”

Section: Chlorophyll Contentmentioning

confidence: 99%

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Chickpea Abiotic Stresses: Combating Drought, Heat and Cold

Kaloki¹,

Devasirvatham²,

Tan³

2019

Abiotic and Biotic Stress in Plants

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Chickpea is an important legume providing dietary proteins to both humans and animals. It also ameliorates soil nitrogen through biological nitrogen fixation. Drought, heat and cold are important factors among abiotic stresses limiting production in chickpea. Identification, validation and integration of agronomic, physiological and biochemical traits into breeding programs could lead to increased rates of genetic gain and the development of better adapted cultivars to abiotic stress conditions. This chapter illustrates the effects of stresses on chickpea growth and development. It also reviews the various traits and their relationship with grain yield under stress and proposes recommendation for future breeding.

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Section: Chlorophyll Contentmentioning

confidence: 99%

Section: Chlorophyll Contentmentioning

confidence: 99%

Chickpea Abiotic Stresses: Combating Drought, Heat and Cold

Kaloki¹,

Devasirvatham²,

Tan³

2019

Abiotic and Biotic Stress in Plants

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show abstract

“…The induced cellular proteolysis in water limitation is a complex process and it is difficult to establish a link between a specific group of proteinase and their corresponding inhibitor families ( Degenkolbe et al, 2009 ; Simova-Stoilova et al, 2010 ; Mosolov and Valueva, 2011 ; Kidric et al, 2014 ). Among different PI families, cysteine PIs (cystatins) are widely studied in relation to water limitation ( Doip et al, 2004 ; Zhang et al, 2008 ; Munger et al, 2012 ; Jangpromma et al, 2014 ; Quain et al, 2014 ; Kunert et al, 2015 ), whereas relatively little is known about the function of KPIs during water limitation ( Downing et al, 1992 ; Kang et al, 2001 ).…”

Section: Introductionmentioning

confidence: 99%

Kunitz Proteinase Inhibitors Limit Water Stress Responses in White Clover (Trifolium repens L.) Plants

et al. 2017

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The response of plants to water deficiency or drought is a complex process, the perception of which is triggered at the molecular level before any visible morphological responses are detected. It was found that different groups of plant proteinase inhibitors (PIs) are induced and play an active role during abiotic stress conditions such as drought. Our previous work with the white clover (Trifolium repens L.) Kunitz Proteinase Inhibitor (Tr-KPI) gene family showed that Tr-KPIs are differentially regulated to ontogenetic and biotic stress associated cues and that, at least some members of this gene family may be required to maintain cellular homeostasis. Altered cellular homeostasis may also affect abiotic stress responses and therefore, we aimed to understand if distinct Tr-PKI members function during drought stress. First, the expression level of three Tr-KPI genes, Tr-KPI1, Tr-KPI2, and Tr-KPI5, was measured in two cultivars and one white clover ecotype with differing capacity to tolerate drought. The expression of Tr-KPI1 and Tr-KPI5 increased in response to water deficiency and this was exaggerated when the plants were treated with a previous period of water deficiency. In contrast, proline accumulation and increased expression of Tr-NCED1, a gene encoding a protein involved in ABA biosynthesis, was delayed in plants that experienced a previous drought period. RNAi knock-down of Tr-KPI1 and Tr-KPI5 resulted in increased proline accumulation in leaf tissue of plants grown under both well-watered and water-deficit conditions. In addition, increased expression of genes involved in ethylene biosynthesis was found. The data suggests that Tr-KPIs, particularly Tr-KPI5, have an explicit function during water limitation. The results also imply that the Tr-KPI family has different in planta proteinase targets and that the functions of this protein family are not solely restricted to one of storage proteins or in response to biotic stress.

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“…Some studies suggest that cystatins participate in the defence against biotic and abiotic stress (Pernas et al 2000;Diop et al 2004;Massonneau et al 2005;Zhang et al 2008). Overexpression of some plant cystatins has conferred osmotic stress tolerance (Jangpromma et al 2014;Quain et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Transcript profiling of serine- and cysteine protease inhibitors inTriticum aestivumvarieties with different drought tolerance

Vaseva

Zehirov

Kirova

et al. 2016

Cereal Research Communications

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A high number of protease inhibitors (PI) have been identified in diverse plant species but information about their role in plant stress responses is still fragmentary. Transcript profiling of six published serine and cysteine protease inhibitor sequences in water-deprived plants from four winter wheat (Triticum aestivum) varieties with varying tolerance was performed in order to outline PIs predominantly accumulating under drought. Expression was analyzed by real time RT-qPCR. Considerable transcript accumulation of Bowman-Birk type PI WALI3 (BBPI) was detected in drought stressed leaves suggesting an important regulatory role of BBPI in adjustment of protein metabolism in leaves under dehydration. Serpin transcripts were less represented in water-deprived plants. Transient accumulation of cystatin transcripts revealed organ-specificity. Under drought cystatin and serpin expression in the leaves of the most drought tolerant variety "Katya" tended to preserve relatively stable levels close to the controls. This preliminary data will serve for future detailed study of regulation of proteolysis in winter wheat subjected to unfavorable environmental factors for development of molecular-based strategies for selection of tolerant varieties.

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Molecular cloning and characterization in eukaryotic expression systems of a sugarcane cysteine protease inhibitor gene involved in drought tolerance

Cited by 11 publications

References 39 publications

Chickpea Abiotic Stresses: Combating Drought, Heat and Cold

Chickpea Abiotic Stresses: Combating Drought, Heat and Cold

Kunitz Proteinase Inhibitors Limit Water Stress Responses in White Clover (Trifolium repens L.) Plants

Transcript profiling of serine- and cysteine protease inhibitors inTriticum aestivumvarieties with different drought tolerance

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