Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs

Porchet, Nicole; Công, Nguyễn Văn; Dufosse, J.; Audie, J P; Guyonnet‐Duperat, Véronique; Gross, Marie-Sylvie; Denis, Cécile V.; Degand, Pierre; Bernheim, Alain; Aubert, Jean‐Pierre

doi:10.1016/0006-291x(91)91580-6

Cited by 377 publications

(202 citation statements)

References 9 publications

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“…The tandem repeat domain, for example, composed of repeats of 17-amino acid units for MUC3 (32), 16 for MUC4 (33), and 169 for MUC6 (34) is characteristic of conventional mucin. However, no such domain containing short repeat units was detected in the molecule of Fc␥BP.…”

Section: Discussionmentioning

confidence: 99%

Human IgGFc Binding Protein (FcγBP) in Colonic Epithelial Cells Exhibits Mucin-like Structure

Harada¹,

Iijima²,

Kobayashi³

et al. 1997

Journal of Biological Chemistry

104

127

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Section: Discussionmentioning

confidence: 99%

Human IgGFc Binding Protein (FcγBP) in Colonic Epithelial Cells Exhibits Mucin-like Structure

Harada¹,

Iijima²,

Kobayashi³

et al. 1997

Journal of Biological Chemistry

104

127

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“…To overcome this problem, we used the first cDNA isolated for MUC4, JER64 (Porchet et al, 1991), to design a mini-MUC4 construct. The JER64 fragment contains a 1.8 kb long MUC4 tandem repeat region, which is composed of the 48 bp motif in repetition.…”

Section: Construction Of the Mini-muc4mentioning

confidence: 99%

“…MUC4 is a high molecular weight O-glycoprotein produced by secretory epithelial cells for the lubrication and protection of ducts and lumen (Porchet et al, 1991;Khorrami et al, 2002). MUC4 is expressed in various epithelial tissues including the trachea, colon, stomach, cervix, and lung (Audie et al, 1993(Audie et al, , 1995.…”

mentioning

confidence: 99%

Human MUC4 mucin induces ultra-structural changes and tumorigenicity in pancreatic cancer cells

et al. 2007

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MUC4 is a type-1 transmembrane glycoprotein and is overexpressed in many carcinomas. It is a heterodimeric protein of 930 kDa, composed of a mucin-type subunit, MUC4a, and a membrane-bound growth factor-like subunit, MUC4b. MUC4 mRNA contains unique 5 0 and 3 0 coding sequences along with a large variable number of tandem repeat (VNTR) domain of 7 -19 kb. A direct association of MUC4 overexpression has been established with the degree of invasiveness and poor prognosis of pancreatic cancer.To understand the precise role of MUC4 in pancreatic cancer, we engineered a MUC4 complementary DNA construct, mini-MUC4, whose deduced protein (320 kDa) is comparable with that of wild-type MUC4 (930 kDa) but represents only 10% of VNTR. Stable ectopic expression of mini-MUC4 in two human pancreatic cancer cell lines, Panc1 and MiaPaCa, showed that MUC4 minigene expression follows a biosynthesis and localisation pattern similar to the wild-type MUC4. Expression of MUC4 resulted in increased growth, motility, and invasiveness of the pancreatic cancer cells in vitro. Ultra-structural examination of MUC4-transfected cells showed the presence of increased number and size of mitochondria. The MUC4-expressing cells also demonstrated an enhanced tumorigenicity in an orthotopic xenograft nude mice model, further supporting a direct role of MUC4 in inducing the cancer properties. In conclusion, our results suggest that MUC4 promotes tumorigenicity and is directly involved in growth and survival of the cancer cells.

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“…To date, eight human mucin genes: MUC1 to MUC4, MUC5AC, MUC5B, MUC6 and MUC7 have been well characterized from various epithelial tissues or mucosae, including airways. [21][22][23][24] MUC4 has been initially isolated from a tracheobronchial mucosa cDNA library by Porchet et al 25 and belongs to the membrane-bound mucin family with MUC1 26 and MUC3. 27 Membrane-bound mucins are expressed in a wide range of mucous and nonmucous-secreting cells.…”

mentioning

confidence: 99%

Diagnostic value of MUC4 immunostaining in distinguishing epithelial mesothelioma and lung adenocarcinoma

et al. 2004

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The distinction between pleural malignant mesothelioma and pleural infiltration by adenocarcinomas has complex therapeutic and medicolegal implications. Although the panel of adenocarcinoma-associated antibodies and one or two mesothelioma markers is useful in this purpose, most of these antibodies are not totally specific. We determined the diagnostic value of MUC4 immunostaining in this issue. MUC4 gene expression was also studied by in situ hybridization and RT-PCR. MUC4 is a membrane-bound mucin that has been suggested to be implicated in malignant progression in humans and rats. The MUC4 gene is expressed in various normal epithelial tissues of endodermic origin and carcinomas. In the respiratory tract, MUC4 transcripts have been detected in normal respiratory epithelium and lung carcinomas. MUC4 protein was expressed in 32 of 35 (91.4%) lung adenocarcinomas on paraffin-embedded tissue. None of the 41 malignant mesotheliomas nor the 32 cases of benign mesothelial cells expressed MUC4 at the protein and mRNA levels. We conclude that MUC4 is a very specific (100%) and sensitive (91.4%) marker of lung adenocarcinomas on paraffin-embedded tissue that could be useful in diagnostic practice in the distinction between malignant mesothelioma and adenocarcinoma.

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Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs

Cited by 377 publications

References 9 publications

Human IgGFc Binding Protein (FcγBP) in Colonic Epithelial Cells Exhibits Mucin-like Structure

Human IgGFc Binding Protein (FcγBP) in Colonic Epithelial Cells Exhibits Mucin-like Structure

Human MUC4 mucin induces ultra-structural changes and tumorigenicity in pancreatic cancer cells

Diagnostic value of MUC4 immunostaining in distinguishing epithelial mesothelioma and lung adenocarcinoma

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