Molecular Cloning and Domain Structure of Human Myosin-VIIa, the Gene Product Defective in Usher Syndrome 1B

Interaction of Arabidopsis kinesin‐like calmodulin‐binding protein with tubulin subunits: modulation by Ca2+‐calmodulin

“…In addition, 1.5 C fusion protein could be detected with S protein (Figure 2b). As shown in Figure 2, highly purified protein was obtained from 2.5 N, 1.5 C and 1.4 C constructs whereas 1.0 C construct yielded highly enriched fusion protein as evident from comparison of Coomassie stained gel of purified protein fraction with that of a blot probed with T7.tag (Chen et al, 1996;Reddy et al, 1996b). MYTH4, a domain present in the tail region of some members of the myosin superfamily (Vlla, IV, X and Xll); CC, s-helical coiled-coil region; MD, motor domain; CB, calmodulin-binding domain.…”

“…The calmodulin-binding domain, mapped to a stretch of 23 amino acids, is located carboxy-terminus to the motor domain. Interestingly, the amino terminal tail domain of KCBPs show significant sequence similarity to a region, referred to as MYTH4 domain, in the tail of some members of myosins (Chen et al, 1996;Reddy et al, 1996b). There is also a region in KCBP that is homologus to talin (Chen et al, 1996).…”

The unconventional myosin-VIIa associates with lysosomes

“…Monoclonal antibodies were generated to amino acids 927-1203 of human myosin-VIIa transcript 2 (accession #U55209; Chen et al 1996). A SacI-XhoI cDNA fragment encoding this region was cloned into pQE30 (Qiagen) to create a HIS-tagged-Myosin-VIIa fusion protein for injection and also cloned into pGEX-2T (Pharmacia) to produce a GST-tagged-MyosinVIIa fusion for use in ELISA assays to test antigen specificity.…”

Myosin genes in Tetrahymena

“…3). Six of the myosins contain the myosin tail homology (MyTH4) [Chen et al, 1996] and/or ezrin, radixin, moesin homology (FERM) motifs [Chishti et al, 1998]. One myosin contains only MyTH4 in the tail domain, and one myosin contains only FERM.…”

“…Four of the Tetrahymena myosins contain both MyTH4 and FERM. The MyTH4/FERM coupling exists in at least four other classes of myosins-VII [Chen et al, 1996, Weil et al, 1996, X [Berg et al, 2000], XII [Berg et al, 2001] and XV [Wang et al, 1998]-and in the divergent myoG in Dictyostelium [Titus, 2003]. In Myosins VII and XV, coupling of FERM and MyTH4 has been duplicated.…”