Molecular cloning and expression analysis of Foxp3 from Nile tilapia

Molecular characterization of the T cell costimulatory receptors CD28 and CTLA4 in the European sea bass

“…Strikingly, the liver of resting pufferfish showed the highest cd28 transcription [21], where this was also related to liver inflammatory response after toxic-induced injury. Strikingly, tilapia liver also showed quite high transcription levels of fox3p [34], the marker for Treg cells, in which the expression of CD28 and CTLA4 is highest in humans. Further evaluation of T cells in fish typical lymphoid tissues, but also in others such as liver, is necessary to have a wider perspective of fish immunity and their regulation.…”

The cellular protein expression of Foxp3 in lymphoid and non-lymphoid organs of Nile tilapia

“…In mammals, studies show that PHA could activate T cells by binding to the T cell receptor/CD3 complex along with the up-regulation of Foxp3 expression [27]. Consistently, several reports indicate that teleost Foxp3 at the mRNA level could also be significantly up-regulated among PBMC with PHA stimulation [16,17,19,20]. In the present study, the frequency of Foxp3 þ cells among PBMC was up to 21.90% after PHA stimulation at 6 h comparing 7.38% in the controls ( Fig.…”

“…Our previous study indicates that the expression of foxp3 mRNA could be detected at moderate level in non-lymphoid tissues as well, such as liver and brain [17]. However, it is unclear whether the expression of foxp3 mRNA at non-lymphoid tissues is of hematopoietic cells or non-hematopoietic cells in the tissue itself.…”

“…The full length of Nile tilapia Foxp3 (F-Foxp3) (377 aa) contains a specific N-terminus and typical motifs of a C2H2 zinc finger domain, a leucine zipper-like domain and a conserved forkhead domain (FHD) [17]. The cDNA sequences encoding the N-terminus of F-Foxp3 (encoding 170 aa) was amplified by RT-PCR with primers containing NdeI/BamHI restriction enzyme sites (forward primer: 5-cgccatatgATGCCAGAGACTTCTGATGAG; reverse primer: 5-cgcggatccTTGAGCAATACTTCTGTCACCAT; small letters are introduced sequences, with NdeI/BamHI sites being underlined), and then inserted into prokaryotic expression vector pET-28a digested with the same restriction enzymes.…”

Missed, Not Missing: Phylogenomic Evidence for the Existence of Avian FoxP3

“…The LZ domain is present in all species having a foxp3 gene, playing an essential role in FoxP3 homodimerization and DNA binding [ 52 ]. The ground tit and coelacanth ( Latimeria chalumnae ) have an isoleucine (I) rather than valine (V) residue at the first position of the published consensus motif V-x(6)-L-x(6)-L-x(6)-L [ 20 ].…”