Molecular cloning and expression analysis of major histocompatibility complex class I, IIA and IIB genes of blunt snout bream (Megalobrama amblycephala)

Luo, Wei; Zhang, Jie; Wen, Jiufu; Liu, Hong; Wang, Weimin; Gao, Zexia

doi:10.1016/j.dci.2013.08.011

Cited by 43 publications

(30 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Discussionmentioning

confidence: 99%

“…The deduced peptide contained typical structural features of the class-including the SP, CP/TM/CYT, and extracellular (α1/α2) domainsconsistent with other species (Cuesta, Esteban, & Meseguer, 2006;Xu, Sun, Shi, Cheng, & Wang, 2011;Luo et al, 2014;Wang, Tan, & Cai, 2015). Moreover, the TM region of PaMHCIIα contained the GxxxGxxGxxxG motif, shared across fishes and mammals (Cosson & Bonifacino, 1992;Xu, Sun, Shi, Cheng, & Wang, 2011;Jiang, Li, Zhang, & Wang, 2013;Luo et al, 2014;Wang, Tan, & Cai, 2015). PaMHCIIα has only one N-linked glycosylation site, consistent with most teleosts (Nath, Kales, Fujiki, & Dixon,2006;Cuesta, Esteban, & Meseguer, 2006;Yu, Ao, & Chen, 2010;Jiang, Li, Zhang, & Wang, 2013;Luo et al, 2014) except zebrafish (Sültmann, Mayer, Figueroa, O'hUigin, & Klein, 1993), channel catfish (Thankappan, Fuller, Godwin, Kearse, & McConnell, 2006), andstickleback (Gasterosteus aculeatus) (Scharsack, Kalbe, & Schaschl,2007).…”

Section: Discussionmentioning

confidence: 99%

“…Stimulation with the trivalent bacterial vaccine or polyinosinic polycytidylic acid (polyI:C) elevated expression in the intestine, kidney, and spleen of large yellow croaker (Yu,Ao, & Chen, 2010). Furthermore, expression increased in the gill, kidney, intestine, and liver of blunt snout bream challenged with Aeromonas hydrophila (Luo et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Pathogen infection or immune stimulation can dramatically increase mRNA expression of MHC class II α (Cuesta, Esteban, & Meseguer, 2006;Yu, Ao, & Chen, 2010;Luo et al, 2014). For example, incubation with heatkilled Saccharomyces cerevisiae or Vibrio anguillarum significantly up-regulated transcription in head-kidney leucocytes of gilthead seabream (Cuesta, Esteban, & Meseguer, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…MHC class II α sequences have been cloned and identified in several teleosts, including gilthead seabream (Sparus aurata) (Cuesta, Esteban, & Meseguer, 2006), large yellow croaker (Larimichthys crocea) (Yu, Ao, & Chen, 2010), Miiuy croaker (Miichthys miiuy) (Xu, Sun, Shi, Cheng, & Wang, 2011), Nile tilapia (Oreochromis niloticus) (Pang et al, 2013), and blunt snout bream (Megalobrama amblycephala) (Luo et al, 2014). Pathogen infection or immune stimulation can dramatically increase mRNA expression of MHC class II α (Cuesta, Esteban, & Meseguer, 2006;Yu, Ao, & Chen, 2010;Luo et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Untitled

Li¹,

Ding²,

Chen³

et al. 2018

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

The MHC (major histocompatibility complex) class II family plays a major role in vertebrate immune systems and is normally found only on antigen-presenting cells. In this study, we identified the gene encoding the α (PaMHCIIα) chain of MHC class II molecules in ayu (Plecoglossus altivelis). Phylogenetic analysis showed that PaMHCIIα was tightly grouped with MHC class IIα in rainbow smelt (Osmerus mordax) and salmon. PaMHCIIα transcripts were detected in all tested tissues, with high levels in the spleen, head kidney, and gill; expression increased significantly upon Vibrio anguillarum infection. PaMHCIIα mRNA expression was also up-regulated in monocytes/macrophages (MO/MФ) after in vitro stimulation with V. anguillarum. We prepared antibodies for detecting N-glycosylation of PaMHCIIα or neutralizing PaMHCIIα on ayu MO/MФ. Western blot analysis showed that native PaMHCIIα was indeed N-glycosylated. Moreover, PaMHCIIα neutralization not only significantly inhibited ayu MO/MФ phagocytosis, but also reduced the ability of V. anguillarum to induce MO/MФ-cytokine expression. Overall, these findings reveal that the important role of PaMHCIIα against bacterial challenge, and it likely contributes to pathogen responsiveness in ayu MO/MФ.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Untitled

Li¹,

Ding²,

Chen³

et al. 2018

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

show abstract

Full-length transcriptome assembly of black amur bream (Megalobrama terminalis) as a reference resource

Liu,

Xie

2024

Mol Biol Rep

View full text Add to dashboard Cite

The first report of diablo in Megalobrama amblycephala: characterization, phylogenetic analysis, functional annotation and expression

Tran

Jakovlić

Wang

2017

J Genet

View full text Add to dashboard Cite

Smac/DIABLO gene is essential for the apoptosis mechanism in mammals. This study is the first report of the Megalobrama amblycephala (ma) diablo gene, and the first report of the tertiary structure of a Diablo polypeptide in fish. Madiablo is 1540-bp long with an open reading frame of 792 bp, encoding a putative protein of 263 amino acids with a molecular weight of 29.2 kDa. Phylogenetic analysis indicates that it is closely related to the zebrafish Diablo-a homologue. It also indicates the existence of two diablo copies (a and b) in teleosts; apart fromthe Percomorpha group,where diablo-b has been lost, but diablo-a had undergone an independent duplication. Madiablo protein contains a long Smac_DIABLO super family domain (Leu32-Asp263) and alpha helices were prevalent in the secondary structure. Homology model of madiablo protein was constructed using the comparative modelling method. Expression of madiablo mRNA transcript was investigated using qPCR: (i) in five tissues from a healthy blunt snout bream, indicating the highest constitutive expression level in liver. (ii) During the embryo and juvenile development, indicating a spike in expression during hatching and in later phases of the juvenile development. (iii) In response to Aeromonas hydrophila infection, indicating the downregulation in liver, spleen and kidney during the first 12 h postinfection and upregulation in spleen and kidney after 24 h postinfection (hpi). The results imply that madiablo is homologous to Diablo orthologues in other species, both structurally and functionally, and that, it probably plays a role in the immune system of M. amblycephala.

show abstract

Molecular cloning and expression analysis of major histocompatibility complex class I, IIA and IIB genes of blunt snout bream (Megalobrama amblycephala)

Cited by 43 publications

References 22 publications

Untitled

Untitled

Full-length transcriptome assembly of black amur bream (Megalobrama terminalis) as a reference resource

The first report of diablo in Megalobrama amblycephala: characterization, phylogenetic analysis, functional annotation and expression

Contact Info

Product

Resources

About