Molecular cloning and expression analysis of scd1 gene from large yellow croaker Larimichthys crocea under cold stress

Xu, Hao; Zhang, Dong Ling; Yu, Debing; Lv, Chang Huan; Luo, Hongyu; Wang, Wenfei

doi:10.1016/j.gene.2015.05.027

Cited by 43 publications

(18 citation statements)

References 40 publications

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“…Palmitoleate and oleate are the main components of monounsaturated fatty acids in membrane phospholipids, triglycerides, cholesterol esters, and ester waxes, which are closely related to membrane fluidity (Nakamura and Nara, 2004). It has been reported that SCD-1 was significantly induced by low temperature stress in orange-spotted grouper (Epinephelus coioides) (Sun et al, 2019) and large yellow croaker (Larimichthys crocea) (Xu et al, 2015). This is consistent with the relatively low temperatures of the natural habitats in Northern China.…”

Section: Crassostrea Gigas Allocates More Energy To Storagesupporting

confidence: 71%

Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata

Wang

et al. 2021

Front. Mar. Sci.

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Understanding the genetic basis of trait variations and their coordination between relative species or populations distributing in different environmental conditions is important in evolutionary biology. In marine ectotherms, growth-defense trade-offs are a common ecological and evolutionary phenomenon. However, the biochemical and molecular mechanisms that govern these trade-offs in marine ectotherms in the evolutionary perspective remain poorly investigated. Oysters are among the most important species in global aquaculture. Crassostrea gigas (C. gigas) and Crassostrea angulata (C. angulata) are two allopatric congeneric dominant oyster species that inhabit the northern and southern intertidal areas of China. Wild C. gigas and C. angulata were spawned, and their F1 progeny were cultured in the same sites to reduce the environmental effects. Untargeted metabolomics and transcriptomics, together with phenotypic parameters including morphological traits (growth performance), nutritional content (glycogen, crude fat, and fatty acid content), physiology (normalized oxygen consumption rate and total antioxidant capacity) were applied to assess metabolic and transcript divergences between C. gigas and C. angulata. Integrated analyses of metabolites and transcriptomes showed that C. gigas allocated more energy to storage and defense by suppressing glycolysis, fatty acid oxidation and by upregulating fatty acid synthesis, antioxidant gene expression, and related metabolites. The metabolic and transcript results were further confirmed by the phenotypic data that C. gigas has higher glycogen and crude fat content and fatty acid unsaturation and stronger antioxidant capacity than C. angulata. In contrast, C. angulata exhibited better growth performance and a higher oxygen consumption rate. These findings suggest that C. angulata allocates more energy to growth, which is embodied in its stronger aerobic capacity and higher levels of protein synthesis genes, metabolites, and growth-related biomarkers. This study will help to enlighten the evolutionary patterns and genetic basis of growth-defense trade-offs in marine ectotherms and the biochemical and molecular mechanisms underlying energy allocation. Also, the key genes and metabolites of glycogen and fatty acids pathway identified in this study will be applied for meat quality improvement in the oyster industry.

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Section: Crassostrea Gigas Allocates More Energy To Storagesupporting

confidence: 71%

Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata

Wang

et al. 2021

Front. Mar. Sci.

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“…Previous studies examining temperature effects on transcription levels of these genes have reported that stearoyl-CoA desaturase plays an important role in the metabolism of membrane fatty acids aiming at regulating membrane fluidity following temperature fluctuations (Tiku et al, 1996;Trueman et al, 2000). Furthermore, the transcription level of scd2 desaturase (Hsieh and Kuo, 2005;Tiku et al, 1996;Trueman et al, 2000;Xu et al, 2015) and elongase (Mellery et al, 2016;Norambuena et al, 2015;Ren et al, 2013) were reported to be upregulated under cold temperature in different species, in agreement with our study.…”

Section: Relationships Between Membrane Fatty Acid and The Transcrisupporting

confidence: 92%

Temperature and metal exposure affect membrane fatty acid composition and transcription of desaturases and elongases in fathead minnow muscle and brain

Fadhlaoui

Pierron

Couture

2018

Ecotoxicology and Environmental Safety

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In this study, we tested the hypothesis that metal exposure affected the normal thermal response of cell membrane FA composition and of elongase and desaturase gene transcription levels. To this end, muscle and brain membrane FA composition and FA desaturase (fads2, degs2 and scd2) and elongase (elovl2, elovl5 and elovl6) gene transcription levels were analyzed in fathead minnows (Pimephales promelas) acclimated for eight weeks to 15, 25 or 30°C exposed or not to cadmium (Cd, 6μg/l) or nickel (Ni, 450 6μg/l). The response of membrane FA composition to temperature variations or metal exposure differed between muscle and brain. In muscle, an increase of temperature induced a decrease of polyunsaturated FA (PUFA) and an increase of saturated FA (SFA) in agreement with the current paradigm. Although a similar response was observed in brain between 15 and 25°C, at 30°C, brain membrane unsaturation was higher than predicted. In both tissues, metal exposure affected the normal thermal response of membrane FA composition. The transcription of desaturases and elongases was higher in the brain and varied with acclimation temperature and metal exposure but these variations did not generally reflect changes in membrane FA composition. The mismatch between gene transcription and membrane composition highlights that several levels of control other than gene transcription are involved in adjusting membrane FA composition, including post-transcriptional regulation of elongases and desaturases and de novo phospholipid biosynthesis. Our study also reveals that metal exposure affects the mechanisms involved in adjusting cell membrane FA composition in ectotherms.

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“…Recent studies that investigated the strengthening of the brain regulatory function in response to salinity and cold stress have indicated that the expression of hormones, neurotransmitters, receptors, and key regulatory proteins was upregulated [13][14][15][16][17][18]. Xu et al [19] investigated the effect of cold exposure on the brain transcriptome of the Yellow rum (Nibea albiflora).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of the brain provides insights into the regulatory mechanism for Coilia nasus migration

Wang

Tang

et al. 2020

BMC Genomics

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Background: Coilia nasus (C. nasus) is an important anadromous fish species that resides in the Yangtze River of China, and has high ecological and economical value. However, wild resources have suffered from a serious reduction in population, attributed to the over-construction of water conservancy projects, overfishing, and environmental pollution. The Ministry of Agriculture and Rural Affairs of the People's Republic of China has issued a notice banning the commercial fishing of wild C. nasus in the Yangtze River. Wild C. nasus populations urgently need to recover. A better understanding of C. nasus migration patterns is necessary to maximize the efficiency of conservation efforts. Juvenile C. nasus experience a simultaneous effect of increasing salinity and cold stress during seaward migration, and the brain plays a comprehensive regulatory role during this process. Therefore, to explore the early seaward migration regulation mechanism of juvenile C. nasus, we performed a comparative transcriptome analysis on the brain of juvenile C. nasus under salinity and cold stress simultaneously. Results: Relevant neurotransmitters, receptors, and regulatory proteins from three categories of regulatory pathway play synergistic regulatory roles during the migration process: neuronal signaling, the sensory system, and environmental adaptation. The significant differential expression of growth-related hormones, thyroid receptors, haptoglobin, and prolactin receptors was similar to the results of relevant research on salmonids and steelhead trout. Conclusions: This study revealed a regulatory network that the brain of juvenile C. nasus constructs during migration, thereby providing basic knowledge on further studies could build on. This study also revealed key regulatory genes similar to salmonids and steelhead trout, thus, this study will lay a theoretical foundation for further study on migration regulation mechanism of anadromous fish species.

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Molecular cloning and expression analysis of scd1 gene from large yellow croaker Larimichthys crocea under cold stress

Cited by 43 publications

References 40 publications

Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata

Integrated Application of Transcriptomics and Metabolomics Reveals the Energy Allocation-Mediated Mechanisms of Growth-Defense Trade-Offs in Crassostrea gigas and Crassostrea angulata

Temperature and metal exposure affect membrane fatty acid composition and transcription of desaturases and elongases in fathead minnow muscle and brain

Transcriptome analysis of the brain provides insights into the regulatory mechanism for Coilia nasus migration

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