Molecular cloning and expression profile of an ATP-binding cassette (ABC) transporter gene from the hemipteran insect Nilaparvata lugens

Zha, Wei; Li, S.H.; Zhou, Liang; Chen, Z.J.; Liu, K.; Yang, Guocai; Hu, Guobin; He, Guang‐Jun; You, Aiqing

doi:10.4238/2015.march.30.26

Cited by 4 publications

(5 citation statements)

References 26 publications

(30 reference statements)

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“…; Zha et al . ); however, it was found that their expression was not sufficiently stable. Conversely, RPS11 was found to be a stably expressed reference gene present at different developmental stages and in different body parts (Yuan et al ., ).…”

Section: Methodsmentioning

confidence: 99%

The β‐N‐acetylhexosaminidase gene family in the brown planthopper, Nilaparvata lugens

Yang

Pan

Zhang

2015

Insect Molecular Biology

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β-N-Acetylhexosaminidases (HEXs) are enzymes that can degrade the chitin oligosaccharides that are produced by the activity of chitinases on chitin in insects. Using bioinformatic methods based on genome and transcriptome databases, 11 β-N-acetylhexosaminidase genes (NlHexs) in Nilaparvata lugens were identified and characterized. Phylogenetic analysis revealed a six-grouped tree topology. The O-Linked N-acetylglucosaminidase (O-GlcNAcase) group includes NlHex11, which harbours a catalytic domain that differs from that of the other 10 NlHexs. Observations of the expression of NlHexs during different developmental stages revealed that NlHex4 is expressed with periodicity during moulting. Although the tissue-specific expression patterns of most NlHexs were nonspecific, NlHex4 was found to be expressed mainly in the female reproductive system as well as in the integument. RNA interference (RNAi) demonstrated failure to shed the old cuticle only in the nymphs treated with double-stranded RNA (dsRNA) targeting NlHex4, and these nymphs eventually died; no observable morphological abnormalities were found in insects treated with dsRNAs targeting the other 10 NlHexs. Based on this study and our previous analyses, a '5 + 1 + 3' pattern of chitinolytic enzymes is proposed, in which five chitinases, one NlHEX and three chitin deacetylases are required for moulting in N. lugens. A better understanding of chitin metabolism in the hemimetabolous insect, N. lugens, would be achieved by considering three chitinolytic enzyme families: chitinase, chitin deacetylase and β-N-acetylhexosaminidase.

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Section: Methodsmentioning

confidence: 99%

The β‐N‐acetylhexosaminidase gene family in the brown planthopper, Nilaparvata lugens

Yang

Pan

Zhang

2015

Insect Molecular Biology

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“…In some insects, P-gps and MRPs are expressed at higher levels in the midgut and Malpighian tubules than in other tissues (Leader and O'Donnell, 2005;Tian et al, 2013;Hull et al, 2014;Guo et al, 2015;Zha et al, 2015), which may result in greater inhibition of P-gp/MRP activity within the digestive system. In the present study, the CR strain treated with chlorpyrifos and verapamil had a two-fold decrease in toxicity compared to the SS.…”

Section: Discussionmentioning

confidence: 99%

“…For example, PxPgp1 is expressed throughout all developmental stages of P. xylostella, and the expression level increases continually throughout development (Tian et al, 2013). Likewise, HaABCC2 and NlABCG expressions increase rapidly with larval growth in Helicoverpa armigera and N. lugens, respectively (Yang, 2013;Zha et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning, Expression Pattern of Multidrug Resistance Associated Protein 1 (Mrp1, Abcc1) Gene, and the Synergistic Effects of Verapamil on Toxicity of Two Insecticides in the Bird Cherry‐oat Aphid

Kang

Zhang

Wang

et al. 2016

Arch Insect Biochem Physiol

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The ATP-binding cassette (ABC) transporters are important transmembrane proteins encoded by a supergene family. The majority of ABC proteins are primary active transporters that bind and hydrolyze ATP to mediate the efflux of a diverse range of substrates across lipid membranes. In this study, we cloned and characterized a putative multidrug resistance associated protein 1 (MRP1) from Rhopalosiphum padi encoded by ABCC1. Structural analysis showed that this protein has structural features typical of the ABC transporter family. Phylogenetic analysis indicated that the amino acid sequence was highly similar that of the corresponding protein from Acyrthosiphon pisum. Real-time quantitative polymerase chain reaction (PCR) analysis showed that ABCC1 was expressed throughout all R. padi developmental stages, with the highest level of expression in the fourth larval instar. We also examined ABCC1 expression in four different tissue types and found that it was most highly expressed in the midgut. Exposing R. padi to imidacloprid and chlorpyrifos increased ABCC1 expression. Furthermore, ABCC1 expression was higher in the imidacloprid-resistant (IR) and chlorpyrifos-resistant (CR) strains than in an insecticide-susceptible strain (SS) of R. padi. Exposing R. padi to verapamil in combination with insecticides significantly increased the toxicity of the insecticides. The respective synergy factor of CR and IR R. padi strain was 1.33 and 1.26, which was lower than that (2.72 and 1.64, respectively) of the SS. Our results clarify the biological function of ABCC1 in R. padi, particularly its role in insecticide resistance, and suggest novel strategies for pest management that use ABC transporter inhibitors to increase the effectiveness of insecticides.

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“…The ATP-binding cassette (ABC) transporters are responsible for import and export of substrates across the membrane in an ATP dependent manner (Nuruzzaman et al 2014;Andolfo et al 2015). ABC family is one of the largest and oldest protein families discovered so far, and is found widely in archaea, bacteria and eukaryotes (Kim et al 2013;Andolfo et al 2015;Zha et al 2015). The typical ABC transporter contains both transmembrane domains (TMD) and ATP-binding domains, which are also called nucleotide-binding domains (NBD) (Zha et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…ABC family is one of the largest and oldest protein families discovered so far, and is found widely in archaea, bacteria and eukaryotes (Kim et al 2013;Andolfo et al 2015;Zha et al 2015). The typical ABC transporter contains both transmembrane domains (TMD) and ATP-binding domains, which are also called nucleotide-binding domains (NBD) (Zha et al 2015). The structures of TMDs are generally not conserved (Smart and Fleming 1996;Van and Tampe 2004;Rea 2007).…”

Section: Introductionmentioning

confidence: 99%

Gene cloning and expression analyses of WBC genes in the developing grapevine seeds

Tang

Wang

Xue

et al. 2018

Journal of Integrative Agriculture

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White-brown complex (WBC) transporters, also called half-size ATP binding cassette G (ABCG) transporters, are involved in many biological processes, including seed development; however, the WBC transporters in grapevines received less attention to date. To reveal the molecular characteristics of WBCs and the connection between WBCs and agronomic traits of stenospermocarpic (seedless) grapevine, we carried out a genomic census and analysis of ovule-associated expression for VvWBC genes in grapevine. We identified 30 VvWBC genes and cloned full-length complementary DNAs (cDNAs) for 20 of these. The tissue or organ-specific expression analysis showed that several VvWBCs exhibited distinct expression patterns with some showing tissue specificity. Twelve VvWBC genes were found to be expressed in the developing ovules. Moreover, the results of quantitative real-time PCR (qRT-PCR) suggested that four of twelve ovule-expressed VvWBCs have distinct expression profiles during the development of ovules between seeded and stenospermocarpic grapevines. These four genes might be involved in ovule abortion. Meanwhile, chromosome mapping, multiple sequence alignments, exon/intron structure analyses and synteny analyses were preformed on VvWBC genes. Our experiments provide a new perspective on the mechanism of stenospermocarpic seedlessness and put forward a framework for further study of WBC transporters.

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Molecular cloning and expression profile of an ATP-binding cassette (ABC) transporter gene from the hemipteran insect Nilaparvata lugens

Cited by 4 publications

References 26 publications

The β‐N‐acetylhexosaminidase gene family in the brown planthopper, Nilaparvata lugens

The β‐N‐acetylhexosaminidase gene family in the brown planthopper, Nilaparvata lugens

Molecular Cloning, Expression Pattern of Multidrug Resistance Associated Protein 1 (Mrp1, Abcc1) Gene, and the Synergistic Effects of Verapamil on Toxicity of Two Insecticides in the Bird Cherry‐oat Aphid

Gene cloning and expression analyses of WBC genes in the developing grapevine seeds

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