Molecular cloning and expression studies of a prolactin receptor in goldfish (Carassius auratus)

Tse, Dicky L.Y.; Chow, Billy K. C.; Chan, Chi-Bun; Lee, Leo Tsz On; Chen, Cheng

doi:10.1016/s0024-3205(99)00632-3

Cited by 58 publications

(39 citation statements)

References 26 publications

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“…The ovary, testis, and headkidney (which has hematopoietic function in fish) also expressed PRLR but at much lower levels (Sandra et al, 1995(Sandra et al, , 2000. A similar distribution of PRLR was also observed in the goldfish (Tse et al, 2000). These results confirmed the results obtained with homologous ligand binding studies in the gills and kidney of tilapia (Auperin et al, 1994) and identified alternative target tissues for PRL in fish.…”

Section: Introductionsupporting

confidence: 81%

“…Despite the presence of all the characteristic features of PRLRs, the amino acid sequence of sbPRLR shares a relatively low level of identity with tiPRLR (Sandra et al, 1995) and gfPRLR (Tse et al, 2000). However, sequence identity is higher (46%) in tilapia and goldfish PRLR.…”

Section: Discussionmentioning

confidence: 98%

“…The similar constitution and organization in sbPRLR, as well as in the other fish, suggest a common function. In the WS box of the ECD, there has been a conservative substitution of the last serine by threonine; an identical substitution is present in goldfish (Tse et al, 2000) and in mouse (Davis and Linzer, 1989) serine has been replaced by glycine. The WS box can be found in all the members of the class 1 superfamily of cytokine receptors with the exception of the growth hormone receptor (GHR), in which conservative substitutions have occurred (Kelly et al, 1991a).…”

Section: Discussionmentioning

confidence: 99%

“…In the goldfish two transcripts, of 3.5 and 4.6 kb, were identified. The 4.6-kb transcript encodes a 600-amino acid protein 48.3% identical to tiPRLR (Tse et al, 2000). The expression of PRLR was analyzed in tilapia by Northern blot and the results obtained demonstrated that the osmoregulatory organs, gills, kidney, and intestine expressed PRLR at higher levels.…”

Section: Introductionmentioning

confidence: 99%

“…PRLRs have also been cloned in an amphibian (Yamamoto et al, 1998), in a euryhaline teleost, Nile tilapia (Oreochromis niloticus) (tiPRLR) (Sandra et al, 1995), and in a fresh water teleost, the goldfish (Carassius auratus) (gf PRLR) (Tse et al, 2000). In tilapia there is a single transcript of 3.2 kb which shares greatest similarity with the long forms of mammalian PRLRs, although the overall homology is low (37%) (Sandra et al, 1995(Sandra et al, , 2000.…”

Section: Introductionmentioning

confidence: 99%

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Cloning, Characterization, and Tissue Distribution of Prolactin Receptor in the Sea Bream (Sparus aurata)

Santos

Ingleton²,

Cavaco

et al. 2001

General and Comparative Endocrinology

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The prolactin receptor (PRLR) was cloned and its tissue distribution characterized in adults of the protandrous hermaphrodite marine teleost, the sea bream (Sparus aurata). An homologous cDNA probe for sea bream PRLR (sbPRLR) was obtained by RT-PCR using gill mRNA. This probe was used to screen intestine and kidney cDNA libraries from which two overlapping clones (1100 and 2425 bp, respectively) were obtained. These clones had 100% sequence identity in the overlapping region (893 bp) and were used to deduce the complete amino acid sequence of sbPRLR. The receptor spans 2640 bp and encodes a protein of 537 amino acids. Features characteristic of PRLR, two pairs of cysteines, WS box, hydrophobic transmembrane domain, box 1, and box 2, were identified and showed a high degree of sequence identity to PRLRs from other vertebrate species. SbPRLR is 29 and 32% identical to tilapia (Oreochromis niloticus) and goldfish (Carassius auratus) PRLRs, respectively. In the sea bream two PRLR transcripts of 2.8 and 3.2 kb were detected in the intestine, kidney, and gills and a single transcript of 2.8 kb was detected in skin and pituitary by Northern blot. Spermiating gonads (more than 95% male tissue; gonado-somatic index of 0.6) contained, in addition to the 2.8-kb transcript, three more transcripts of 1.9, 1.3, and 1.1 kb. RT-PCR, which is a far more sensitive method than Northern blot, detected PRLR mRNA in gills, intestine, brain, pituitary, kidney, liver, gonads, spleen, head-kidney, heart, muscle, and bone. Immunohistochemistry using specific polyclonal antibodies raised against an oligopeptide from the extracellular domain of sbPRLR detected PRLR in several epithelial tissues of juvenile sea bream, including the anterior gut, renal tubule, choroid membrane of the third ventricle, saccus vasculosus, branchial chloride cells, and branchial cartilage.

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Section: Introductionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cloning, Characterization, and Tissue Distribution of Prolactin Receptor in the Sea Bream (Sparus aurata)

Santos

Ingleton²,

Cavaco

et al. 2001

General and Comparative Endocrinology

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Seasonal environmental changes modulate the prolactin receptor expression in an eurythermal fish

Martin

Cáceres

Azócar

et al. 2004

J of Cellular Biochemistry

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Eurythermal fish have evolved compensatory responses to the cyclical seasonal changes of the environment. The complex adaptive mechanisms include the transduction of the physical parameters variations into molecular signals. Studies in carp have indicated that prolactin and growth hormone expression is associated with acclimatization, suggesting that the pituitary gland is a relevant physiological node in the generation of the homeostatic rearrangement that occurs in this adaptive process. Here, we report the cloning and characterization of a full-length carp prolactin receptor cDNA, which codes for the long form of the protein resembling that found in mammalian prolactin receptors. We identified up to three receptor transcript isoforms in different tissues of the teleost and assessed cell- and temporal-specific transcription and protein expression in carp undergoing seasonal acclimatization. The distinctive pattern of expression that carp prolactin receptor (cPRLr) depicts upon seasonal acclimatization supports the hypothesis that prolactin and its receptor are clearly involved in the new homeostatic stage that the eurythermal fish needs to survive during the cyclical changes of its habitat.

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Gene structure and seasonal expression of carp fish prolactin short receptor isoforms

Martin¹,

Hurtado²,

Quezada³

et al. 2006

J of Cellular Biochemistry

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The complex adaptive mechanisms that eurythermal fish have evolved in response to the seasonal changes of the environment include the transduction of the physical parameter variations into neuroendocrine signals. Studies in carp (Cyprinus carpio) have indicated that prolactin (PRL) and growth hormone (GH) expression is associated with acclimatization, suggesting that the pituitary gland is a relevant physiological node in this adaptive process. Also, the distinctive pattern of expression that carp prolactin receptor (PRLr) protein depicts upon seasonal acclimatization supports the hypothesis that PRL and its receptor clearly are involved in the new homeostatic stage that the eurythermal fish needs to survive during the cyclical changes of its habitat. Here, we characterize the first prolactin receptor gene in a teleost and show that its expression is not associated with alternative promoters, unlike in humans and rodents. Using the regulatory region to direct the transcription of green fluorescent protein (GFP) in zebrafish embryos, we mapped the appearance of this hormone receptor during fish development. This is the first report identifying a fish prolactin receptor gene expressing transcript isoforms encoding for short forms of the protein (45 kDa). These have been found in osmoregulatory tissues of the carp and are regulated in connection with the seasonal acclimatization of the fish.

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Molecular cloning and expression studies of a prolactin receptor in goldfish (Carassius auratus)

Cited by 58 publications

References 26 publications

Cloning, Characterization, and Tissue Distribution of Prolactin Receptor in the Sea Bream (Sparus aurata)

Cloning, Characterization, and Tissue Distribution of Prolactin Receptor in the Sea Bream (Sparus aurata)

Seasonal environmental changes modulate the prolactin receptor expression in an eurythermal fish

Gene structure and seasonal expression of carp fish prolactin short receptor isoforms

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