Molecular cloning and functional analysis of nucleotide-binding oligomerization domain 1 (NOD1) in olive flounder, Paralichthys olivaceus

Park, Seong Bin; Hikima, Jun-ichi; Suzuki, Yoshiaki; Ohtani, Maki; Nho, Seong Won; Seok, In; Jang, Ho G.; Kondo, Hidehiro; Hirono, Ikuo; Aoki, Takashi; Jung, Taehun

doi:10.1016/j.dci.2011.11.007

Cited by 51 publications

(20 citation statements)

References 29 publications

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“…While NOD1 is not classically associated with RNA recognition, our data are concordant with the recently reported increase in NOD1 and NOD2 signaling initiated by either poly(I·C) or infection with RNA viruses, for example, viral hemorrhagic septicemia virus (VHSV) as well as vesicular stomatitis virus (VSV) and murine norovirus-1 (MNV-1) (26,27). In the study by Park et al (27), activation of NOD1 and -2 in macrophages was primed by type I IFN, which was synthesized through the classical antiviral innate immunity pathways.…”

Section: Discussionsupporting

confidence: 76%

“…In the study by Park et al (27), activation of NOD1 and -2 in macrophages was primed by type I IFN, which was synthesized through the classical antiviral innate immunity pathways. In contrast to our results, NOD activation by RNA was only revealed following exposure to bacterial PAMPs.…”

Section: Discussionmentioning

confidence: 99%

“…Until recently, NOD1 and NOD2 were thought to be activated specifically in response to bacterial infection through recognition of peptides derived from the degradation of constituents of the bacterial wall (20). Recent reports suggest that both receptors might also respond to viral infections, thus participating in the coordinated host defense against viruses (26)(27)(28)(29). In the context of viral infections, activation of both NOD1 and NOD2 has been reported to depend on type I IFN, synthesized as a result of activation of other PRRs.…”

Section: It Is Thus Of Considerable Interest To Investigate What Initmentioning

confidence: 99%

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NOD1 Participates in the Innate Immune Response Triggered by Hepatitis C Virus Polymerase

Vegna

Grégoire

Moreau

et al. 2016

J Virol

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Hepatitis C virus (HCV) triggers innate immunity signaling in the infected cell. Replication of the viral genome is dispensable for this phenotype, and we along with others have recently shown that NS5B, the viral RNA-dependent RNA polymerase, synthesizes double-stranded RNA (dsRNA) from cellular templates, thus eliciting an inflammatory response, notably via activation of type I interferon and lymphotoxin ␤. Here, we investigated intracellular signal transduction pathways involved in this process. Using HepaRG cells, a model that largely recapitulates the in vivo complexities of the innate immunity receptor signaling, we have confirmed that NS5B triggered increased expression of the canonical pattern recognition receptors (PRRs) specific for dsRNA, namely, RIG-I, MDA5, and Toll-like receptor 3 (TLR3). Unexpectedly, intracellular dsRNA also led to accumulation of NOD1, a receptor classically involved in recognition of bacterial peptidoglycans. NOD1 activation, confirmed by analysis of its downstream targets, was likely due to its interaction with dsRNA and was independent of RIG-I and mitochondrial antiviral signaling protein (MAVS/IPS-1/Cardif/VISA) signaling. It is likely to have a functional significance in the cellular response in the context of HCV infection since interference with the NOD1 pathway severely reduced the inflammatory response elicited by NS5B. IMPORTANCEIn this study, we show that NOD1, a PRR that normally senses bacterial peptidoglycans, is activated by HCV viral polymerase, probably through an interaction with dsRNA, suggesting that NOD1 acts as an RNA ligand recognition receptor. In consequence, interference with NOD1-mediated signaling significantly weakens the inflammatory response to dsRNA. These results add a new level of complexity to the understanding of the cross talk between different classes of pattern recognition receptors and may be related to certain complications of chronic hepatitis C virus infection.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: It Is Thus Of Considerable Interest To Investigate What Initmentioning

confidence: 99%

See 1 more Smart Citation

NOD1 Participates in the Innate Immune Response Triggered by Hepatitis C Virus Polymerase

Vegna

Grégoire

Moreau

et al. 2016

J Virol

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“…However, the majority of other fish NACHT-containing proteins are more related to each other than to mammalian NLR proteins (Stein et al, 2007;Laing et al, 2008). With respect to function, only the antibacterial roles of fish NOD1 and NOD2 were revealed in teleost fish (Oehlers et al, 2011;Park et al, 2012). In the present study, we identified two other members of NACHT-family in zebrafish, which contain only one NACHT domain.…”

Section: Introductionmentioning

confidence: 71%

“…The E. tarda invasion assay was performed according to the previous report (Park et al, 2012) with minor modification. Stable ZF4 cells transfected respectively with ptGFP1-NALPL1, ptGFP1-NAL-PL2 and ptGFP1 were seeded at 3 Â 10 5 cells per well at 12-well plates overnight.…”

Section: E Tarda Invasion Assaymentioning

confidence: 99%

Expression and protective role of two novel NACHT-containing proteins in pathogen infection

Hu¹,

Zhang²,

Xue³

et al. 2014

Developmental & Comparative Immunology

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The Innate Immune Response in Fish

Jørgensen¹

2014

Fish Vaccination

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Molecular cloning and functional analysis of nucleotide-binding oligomerization domain 1 (NOD1) in olive flounder, Paralichthys olivaceus

Cited by 51 publications

References 29 publications

NOD1 Participates in the Innate Immune Response Triggered by Hepatitis C Virus Polymerase

NOD1 Participates in the Innate Immune Response Triggered by Hepatitis C Virus Polymerase

Expression and protective role of two novel NACHT-containing proteins in pathogen infection

The Innate Immune Response in Fish

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