Molecular cloning and functional characterization of a DNA damage-inducible (DDI) gene in Arabidopsis

Ding, Lei; Yang, Guo‐Yu; Cao, Jun; Zhou, Yang; Yang, Ruiying

doi:10.1016/j.pmpp.2016.06.001

Cited by 5 publications

(5 citation statements)

References 51 publications

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“…Overexpression of Ta-Ub2 in tobacco resulted in high tolerance to drought (Guo et al, 2008) and the deletion of AtDDI1 increased susceptibility to pathogenic bacteria (Ding et al, 2016). In this study, transgenic Arabidopsis lines overexpressing CsDDI1 showed higher Fv/Fm ratios ( Figures 6A, B), lower EL ( Figure 6C), and higher leaf growth rate ( Figures S4G, H).…”

Section: Discussionsupporting

confidence: 51%

“…Plants have evolved the ability to cope with various environmental stresses to ensure survival and proliferation, including solar UV and ionizing radiation, chemical mutagens, heavy metals, droughts, heat, pathogenic attacks, and chilling. Although different environmental stresses may cause different disorders or symptoms in plants, they can all cause cellular DNA damage (Durrant et al, 2007;Gichner et al, 2008;Ciccia and Elledge, 2010;Achary et al, 2012;Cvjetko et al, 2014;Roy, 2014;Daghino et al, 2016;Ding et al, 2016;Maric et al, 2017;Shim et al, 2018). DNA damage may result in changes to both the chemical and physical structures of DNA, which can seriously threaten the survival and the faithful transmission of genetic information in plants (Zhang et al, 2015a;Ding et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…DNA damage-inducible (DDI) proteins are usually related to plant defense responses and play important roles in DNA repair pathways (Maric et al, 2017). Arabidopsis DNA damage-inducible protein 1 (AtDDI1) participates in plant defense responses against abiotic stresses such as drought and salt by regulating the expression of defense genes (Ding et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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DNA Damage Inducible Protein 1 is Involved in Cold Adaption of Harvested Cucumber Fruit

Wang

Zhu

2020

Front. Plant Sci.

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Chilling stress can cause cellular DNA damage, affecting the faithful transmission of genetic information. Cold acclimation enhances chilling tolerance, but it is not clear that the process of cold adaption involves DNA damage responses, as cold acclimation does not form real chilling stress. Here we showed with cucumber fruit that pre-storage cold acclimation (PsCA) reduces chilling injury and upregulates DNA damage inducible protein1 (CsDDI1), suggesting that the chilling tolerance induced by cold acclimation involves CsDDI1 transcription. Application of nitric oxide (NO), abscisic acid (ABA) or H 2 O 2 biosynthesis inhibitor before PsCA treatment downregulates CsDDI1 and aggravates chilling injury, while H 2 O 2 generation inhibition plus exogenous NO or ABA application before PsCA treatment restores chilling tolerance, but does not restore CsDDI1 expression, suggesting H 2 O 2 plays a crucial role in triggering cold adaption. CsDDI1 overexpression Arabidopsis lines show faster growth, stronger chilling tolerance, lower reactive oxygen species levels, enhanced catalase and superoxide dismutase activities and higher expression of nine other Arabidopsis defense genes under chilling stress, suggesting CsDDI1 strengthens defenses against chilling stress by enhancing antioxidant defense system. Taken together, CsDDI1 positively regulates chilling tolerance induced by cold acclimation in cucumber. In addition, H 2 O 2 is involved in initiation of cold acclimation. While CsDDI1 upregulation requires H 2 O 2 as a key signaling molecule, the upregulation of CsDDI1 activates an antioxidant system to reduce biotoxic accumulation of H 2 O 2 and helps in DNA repair.

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Section: Discussionsupporting

confidence: 51%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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DNA Damage Inducible Protein 1 is Involved in Cold Adaption of Harvested Cucumber Fruit

Wang

Zhu

2020

Front. Plant Sci.

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“…The repair of DNA damage is essential for living organisms under biotic stress as well as abiotic stress. DDI proteins are DNA repair proteins, which can also regulate gene expression during plant defense responses (Ding et al, 2016 ). In the present study, a DDI protein 1 isoform (spot 4) was found to be up-regulated by PsCA, suggesting PsCA activated the DNA repair mechanism, which makes it possible to keep DNA intact and is especially important for cucumber fruit as a reproductive organ.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Differentially Accumulated Proteins in Cucumber (Cucumis sativus) Fruit Peel in Response to Pre-storage Cold Acclimation

2018

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Harvested fruits are still living organs and respond to environmental stimuli. Low temperature storage is effective in extending life of harvested fruit, but it may also cause chilling injury. Cold acclimation has been shown to induce chilling tolerance in plants, but what proteomic changes caused by cold acclimation are related to defense against chilling stress remains largely unclear. Here, 3 d of pre-storage cold acclimation (PsCA) at 10°C reduced chilling injury and secondary disease severity in cucumber stored at 5°C by 51 and 94%, respectively, compared with the control which was directly stored at 5°C. Proteomic analysis of cucumber peel identified 21 significant differentially-accumulated proteins (SDAPs) right after PsCA treatment and 23 after the following cold storage (PsCA+CS). These proteins are mainly related to stress response and defense (SRD), energy metabolism, protein metabolism, signal transduction, primary metabolism, and transcription. The SRD proteins, which made up 37% of the 21 and 47% of the 23, respectively, represented the largest class of SDAPs, and all but one protein were up-regulated, suggesting accumulation of proteins involved in defense response is central feature of proteomic profile changes brought about by PsCA. In fruit just after PsCA treatment, the identified SDAPs are related to responses to various stresses, including chilling, salt stress, dehydration, fungi, bacteria, insects, and DNA damage. However, after prolonged cold storage, the targeted proteins in acclimated fruit were narrowed down in scope to those involved in defense against chilling and pathogens. The change patterns at the transcription level of the majority of the up-regulated differentially-accumulated proteins were highly consistent with those at protein level. Taken all, the results suggest that the short-time cold acclimation initiated comprehensive defense responses in cucumber fruit at first, while the long term storage thereafter altered the responses more specifically to chilling. These findings add to the understanding of plants' molecular responses to cold acclimation.

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“…Higher FPKM value of DNA damage-inducible protein-1 in anhydrobiotic IJs signifies its importance in desiccation stress tolerance as they are reported to play a crucial role in DNA repair pathways in C . elegans [ 31 – 32 ] and Arabidopsis [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Deciphering the mechanism of anhydrobiosis in the entomopathogenic nematode Heterorhabditis indica through comparative transcriptomics

Balakumaran

Chidambaranathan²,

P.³

et al. 2022

PLoS ONE

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The entomopathogenic nematode, Heterorhabditis indica, is a popular biocontrol agent of high commercial significance. It possesses tremendous genetic architecture to survive desiccation stress by undergoing anhydrobiosis to increase its lifespan—an attribute exploited in the formulation technology. The comparative transcriptome of unstressed and anhydrobiotic H. indica revealed several previously concealed metabolic events crucial for adapting towards the moisture stress. During the induction of anhydrobiosis in the infective juveniles (IJ), 1584 transcripts were upregulated and 340 downregulated. As a strategy towards anhydrobiotic survival, the IJ showed activation of several genes critical to antioxidant defense, detoxification pathways, signal transduction, unfolded protein response and molecular chaperones and ubiquitin-proteasome system. Differential expression of several genes involved in gluconeogenesis - β-oxidation of fatty acids, glyoxylate pathway; glyceroneogenesis; fatty acid biosynthesis; amino-acid metabolism - shikimate pathway, sachharopine pathway, kyneurine pathway, lysine biosynthesis; one-carbon metabolism—polyamine pathway, transsulfuration pathway, folate cycle, methionine cycle, nucleotide biosynthesis; mevalonate pathway; and glyceraldehyde-3-phosphate dehydrogenase were also observed. We report the role of shikimate pathway, sachharopine pathway and glyceroneogenesis in anhydrobiotes, and seven classes of repeat proteins, specifically in H. indica for the first time. These results provide insights into anhydrobiotic survival strategies which can be utilized to strengthen the development of novel formulations with enhanced and sustained shelf-life.

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Molecular cloning and functional characterization of a DNA damage-inducible (DDI) gene in Arabidopsis

Cited by 5 publications

References 51 publications

DNA Damage Inducible Protein 1 is Involved in Cold Adaption of Harvested Cucumber Fruit

DNA Damage Inducible Protein 1 is Involved in Cold Adaption of Harvested Cucumber Fruit

Proteomic Analysis of Differentially Accumulated Proteins in Cucumber (Cucumis sativus) Fruit Peel in Response to Pre-storage Cold Acclimation

Deciphering the mechanism of anhydrobiosis in the entomopathogenic nematode Heterorhabditis indica through comparative transcriptomics

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