Molecular cloning and genomic characterization of novel Leptin-like genes in salmonids provide new insight into the evolution of the Leptin gene family

Angotzi, Anna Rita; Stefansson, Sigurd O.; Nilsen, Tom Ole; Rathore, Raja Mansingh; Rønnestad, Ivar

doi:10.1016/j.ygcen.2013.03.022

Cited by 45 publications

(33 citation statements)

References 76 publications

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“…(JX131306), respectively (Angotzi et al, 2013). Thus, the assay primarily measures LepA1, although based on sequence similarities some cross-reactivity might exist with LepA2, but not LepB1.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Among these, leptin and ghrelin together with their corresponding functional receptors, the long isoform leptin receptor (LepRL) and the growth hormone secretagogue receptor 1a (GHS-R1a), respectively have been identified in a number of fish species including salmonids (Angotzi et al, 2013;Gong et al, 2013;Jönsson, 2013;Kaiya et al, 2009a;Rønnestad et al, 2010). (Kim et al, 2008;Wagoner et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Roles of leptin and ghrelin in adipogenesis and lipid metabolism of rainbow trout adipocytes in vitro

Salmerón

Johansson

Asaad

et al. 2015

Comparative Biochemistry and Physiology Part A: Molecular &

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Section: Accepted Manuscriptmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Roles of leptin and ghrelin in adipogenesis and lipid metabolism of rainbow trout adipocytes in vitro

Salmerón

Johansson

Asaad

et al. 2015

Comparative Biochemistry and Physiology Part A: Molecular &

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“…Since leptin physiology is highly conserved among fish species [105,108], zebrafish studies may be fostered by findings from other fishes. For instance, like zebrafish, salmon contain genes encoding for leptin-a and leptin-b (the latter closely resembling mammalian leptin [180]). While a long-term diet does not affect leptin expression in salmon, the short-term diet up-regulates both lepa1 and lepa2 (encoding for leptin-a1 and leptin-a2) [181].…”

Section: Lessons From Other Fish Speciesmentioning

confidence: 99%

Developing ‘integrative’ zebrafish models of behavioral and metabolic disorders

Nguyen

Yang

Neelkantan

et al. 2013

Behavioural Brain Research

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“…Through gene or genome-wide duplication events, multiple forms of leptin exist in fishes (Huising et al, 2006b) with the major form, leptin A (LepA) produced predominantly in the liver in most species including the tilapia (Angotzi et al, 2013; Douros et al, 2014; Trombley et al, 2012b). Evidence suggests that leptin increases in response to various stressors (e.g.…”

Section: Introductionmentioning

confidence: 99%

Control of leptin by metabolic state and its regulatory interactions with pituitary growth hormone and hepatic growth hormone receptors and insulin like growth factors in the tilapia ( Oreochromis mossambicus )

Douros

Baltzegar

Mankiewicz

et al. 2017

General and Comparative Endocrinology

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Leptin is an important cytokine for regulating energy homeostasis, however, relatively little is known about its function and control in teleost fishes or other ectotherms, particularly with regard to interactions with the growth hormone (GH)/insulin-like growth factors (IGFs) growth regulatory axis. Here we assessed the regulation of LepA, the dominant paralog in tilapia (Oreochromis mossambicus) and other teleosts under altered nutritional state, and evaluated how LepA might alter pituitary growth hormone (GH) and hepatic insulin-like growth factors (IGFs) that are known to be disparately regulated by metabolic state. Circulating LepA, and lepa and lepr gene expression increased after 3-weeks fasting and declined to control levels 10 days following refeeding. This pattern of leptin regulation by metabolic state is similar to that previously observed for pituitary GH and opposite that of hepatic GHR and/or IGF dynamics in tilapia and other fishes. We therefore evaluated if LepA might differentially regulate pituitary GH, and hepatic GH receptors (GHRs) and IGFs. Recombinant tilapia LepA (rtLepA) increased hepatic gene expression of igf-1, igf-2, ghr-1, and ghr-2 from isolated hepatocytes following 24 h incubation. Intraperitoneal rtLepA injection, on the other hand, stimulated hepatic igf-1, but had little effect on hepatic igf-2, ghr1, or ghr2 mRNA abundance. LepA suppressed GH accumulation and gh mRNA in pituitaries in vitro, but had no effect on GH release. We next sought to test if abolition of pituitary GH via hypophysectomy (Hx) affects the expression of hepatic lepa and lepr. Hypophysectomy significantly increases hepatic lepa mRNA abundance, while GH replacement in Hx fish restores lepa mRNA levels to that of sham controls. Leptin receptor (lepr) mRNA was unchanged by Hx. In in vitro hepatocyte incubations, GH inhibits lepa and lepr mRNA expression at low concentrations, while higher concentration stimulates lepa expression. Taken together, these findings indicate LepA gene expression and secretion increases with fasting, consistent with the hormones function in promoting energy expenditure during catabolic stress. It would also appear that LepA might play an important role in stimulating GHR and IGFs to potentially spare declines in these factors during catabolism. Evidence also suggests for the first time in teleosts that GH may exert important regulatory effects on hepatic LepA production, insofar as physiological levels(0.05–1 nM) suppresse lepa mRNA accumulation. Leptin A, may in turn exert negative feedback effects on basal GH mRNA abundance, but not secretion.

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Molecular cloning and genomic characterization of novel Leptin-like genes in salmonids provide new insight into the evolution of the Leptin gene family

Cited by 45 publications

References 76 publications

Roles of leptin and ghrelin in adipogenesis and lipid metabolism of rainbow trout adipocytes in vitro

Roles of leptin and ghrelin in adipogenesis and lipid metabolism of rainbow trout adipocytes in vitro

Developing ‘integrative’ zebrafish models of behavioral and metabolic disorders

Control of leptin by metabolic state and its regulatory interactions with pituitary growth hormone and hepatic growth hormone receptors and insulin like growth factors in the tilapia ( Oreochromis mossambicus )

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