Molecular cloning and mRNA expression of the vitellogenin and nuclear receptor gene induced by 17β-estradiol in the mud carp, Cirrhinus molitorella

Liang, Yue; Fang, Zhanqiang

doi:10.1007/s10646-011-0831-x

Cited by 9 publications

(6 citation statements)

References 52 publications

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“…The up-regulation of era and vtg mRNA levels was observed in all treatment groups after 20 and 40 days of exposure in the livers. It is reported that the expression of era and vtg demonstrated a significant relationship after E2 exposure in the liver of mud carp [45]. In female zebrafish, the increase of plasma E2 was accompanied by up-regulated hepatic era and vtg expression after exposure to fluorotelomer alcohol (6:2 FTOH) [46].…”

Section: Discussionmentioning

confidence: 99%

Butachlor causes disruption of HPG and HPT axes in adult female rare minnow (Gobiocypris rarus)

Zhu

Zha

et al. 2014

Chemico-Biological Interactions

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a b s t r a c tButachlor is a chloroacetamide herbicide widely used in Asia, and may enter the aquatic environment through agricultural application. In this study, plasma VTG and hormone levels (E2, 11-KT, T3 and T4) were determined after the female rare minnow (Gobiocypris rarus) was exposed to butachlor at environmental relevant concentrations (0, 0.1, 1, and 10 lg/L) for 40 days. The mRNA levels of the HPG axisrelated genes (gnrh, era, vtg, star, lhr, 3b-hsd, cyp11a, cyp17, cyp19a and cyp19b), and the HPT axis-related genes (tra, dio1, dio2, and dio3) were quantified after 20 and 40 days exposure to butachlor. For the HPG axis, the plasma 11-KT was increased at exposure concentration of 10 lg/L, and VTG was significantly decreased at 1 lg/L. Functional genes like gnrh and cyp19b in the brains, star, lhr, cyp11a, 3b-hsd, and cyp19a in the ovaries, and era and vtg in livers were up-regulated. For the HPT axis, the results showed that plasma T4 levels were significantly increased, the gene expression of dio1 was up-regulated, dio2 showed no significant variation, and dio3 was down-regulated in the livers. These results indicated that butachlor may promote the accumulation of T4 in fish through inactive deiodinase type 3. The transcription of HPG axis-related genes could serve as an auto-regulation of hormone levels after exposure to butachlor. Furthermore, the activation of gnrh may play an important role as a feed-back mechanism in the regulation of hormone levels and crosstalk of endocrine axes.

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Section: Discussionmentioning

confidence: 99%

Butachlor causes disruption of HPG and HPT axes in adult female rare minnow (Gobiocypris rarus)

Zhu

Zha

et al. 2014

Chemico-Biological Interactions

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“…However, the effects of E2 or EE2 on Ru-Vg gene expression are significantly lower compared to those seen in other fish. In fish such as the slender bitterling, mud carp, zebrafish, and rainbow trout, the levels of hepatocyte Vg mRNA or plasma Vg were induced on a logarithmic scale by E2 or EE2 treatments [42,43,44,45]. In contrast, VgAo1 mRNA levels in the hepatocytes of zebrafish and white cloud mountain minnow were only induced approximately 1.5- and 10-fold by E2 treatment [34,36].…”

Section: Resultsmentioning

confidence: 99%

“…As shown Figure 6, the 5'-flanking region of Ru-Vg was activated by the expression of ERα, and this expression was synergistically increased by ERα together with 10 –7 M E2 or 10 –7 M EE2. These results suggest that Ru-Vg expression can be regulated by estrogen derivatives and indicate that R. uyekii and the Ru-Vg gene may have utility as biomarkers for exposure to endocrine-disrupting chemicals (EDCs), which can be harmful to human health by disrupting the endocrine, reproductive, and immune systems [42,48]. …”

Section: Resultsmentioning

confidence: 99%

Characterization, Expression Profile, and Promoter Analysis of the Rhodeus uyekii Vitellogenin Ao1 Gene

Kong

Kim

Moon

et al. 2014

IJMS

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The fish Vitellogenin (Vg) gene has been applied as a biomarker for exposure to estrogenic compounds in the aquatic environment. In this study, we cloned and characterized Vg cDNA from the Korean rose bitterling Rhodeus uyekii (Ru-Vg). The Ru-Vg cDNA encodes a 1424-amino-acid polypeptide that belongs to the VgAo1 family and contains a putative signal peptide, lipovitellin I, phosvitin, and lipovitellin II, but does not contain the vWFD domain or the C-terminal peptide. The deduced Ru-Vg protein has high amino acid identity (73.97%–32.17%) with fish Vg proteins. Pairwise alignment and phylogenetic analysis revealed that Ru-Vg is most closely related to Acheilognathus yamatsutae Vg. Ru-Vg transcripts were detected using quantitative polymerase chain reaction in all tissues tested, with the highest level of expression observed in the ovary. Ru-Vg mRNA was upregulated in R. uyekii hepatopancreas cells in response to treatment with 17β-estradiol (E2) or 17α-ethinylestradiol (EE2). Luciferase reporter expression, driven by the 5'-regulatory region of the Ru-Vg gene spanning from −1020 bp to the start codon was induced by the estrogen receptor and was synergistically activated by treatment with E2 or EE2. These results suggest that R. uyekii and the Ru-Vg gene may be useful as biomarkers for exposure to E2 or EE2.

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“…In this study, we did not find any other cDNA encoding other types of Vtg. To date, a single vtg form has been also found in another cyprinid species, C. molitorella (Liang & Fang, 2012). Miracle et al (2006) found that vtg1 induction levels in male fathead minnow (P. promelas)…”

Section: Discussionmentioning

confidence: 96%

Vitellogenin expression in wild cyprinid Petroleuciscus esfahani as a biomarker of endocrine disruption along the Zayandeh Roud River, Iran

et al. 2016

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Aquatic environments are the ultimate sink for most of anthropogenic pollutants. The Zayandeh Roud River is the most important river in the central Iranian Plateau, supplying water to a large population. In order to determine the potential occurrence and in vivo effects of Endocrine Disrupting Chemicals (EDCs) with estrogenic or anti-androgenic properties we analyzed the wild populations of an extensively distributed endemic fish species, Petroleuciscus esfahani. For this purpose, specimens were caught from two sites upstream and two sites downstream of the expected major anthropogenic pollution sources. P. esfahani full-length cDNAs for vitellogenin (vtg), with 4177 base pairs (bp) encoding a 1339 amino acids (aa), and for β-actin (actb), with 1776 bp encoding a 375 aa, were amplified and cloned. Hepatic vtg mRNA expression levels were measured by quantitative real-time PCR. Condition factor, gonadosomatic index and sex ratio were calculated and compared with vtg expression. Gonad histology was performed to study the possible presence of intersex condition. Detection of vtg transcripts in male individuals from the two downstream sampling sites supports the hypothesis of exposure to EDCs in these regions. Higher vtg expression in male individuals, together with reduced gonad size and condition factor, in specimens from the site located downstream of the major steel mill plant suggest a major endocrine disruption in this area.

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Molecular cloning and mRNA expression of the vitellogenin and nuclear receptor gene induced by 17β-estradiol in the mud carp, Cirrhinus molitorella

Cited by 9 publications

References 52 publications

Butachlor causes disruption of HPG and HPT axes in adult female rare minnow (Gobiocypris rarus)

Butachlor causes disruption of HPG and HPT axes in adult female rare minnow (Gobiocypris rarus)

Characterization, Expression Profile, and Promoter Analysis of the Rhodeus uyekii Vitellogenin Ao1 Gene

Vitellogenin expression in wild cyprinid Petroleuciscus esfahani as a biomarker of endocrine disruption along the Zayandeh Roud River, Iran

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