Molecular cloning and mRNA expression analysis of antizyme inhibitor 1 in the ovarian follicles of the Sichuan white goose

Ma, Rong; Jiang, Dongmei; Kang, Bo; Bai, Lin; He, Hua; Chen, Ziyu; Zhang, Yi

doi:10.1016/j.gene.2015.05.014

Cited by 7 publications

(3 citation statements)

References 30 publications

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“…AZIN1 expression pattern has been correlated to genes involved in the rat oestrous cycle and avian ovarian follicles, suggesting a possible role of AZIN1 in reproductive physiology. 58,59 Additionally, AZIN1 is highly expressed in vasopressinergic and oxytocinergic neurons of the male rat hypothalamus. AZIN expression in hypothalamic nuclei is directly correlated to increased polyamines resulting in transcriptional regulation of the neuropeptide hormone arginine vasopressin.…”

Section: Physiological Role Of Antizyme Inhibitors Physiological Rolementioning

confidence: 99%

Role of Antizyme Inhibitor Proteins in Cancers and Beyond

Tulluri

Nemmara

2021

OTT

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Polyamines are multivalent organic cations essential for many cellular functions, including cell growth, differentiation, and proliferation. However, elevated polyamine levels are associated with a slew of pathological conditions, including multiple cancers. Intracellular polyamine levels are primarily controlled by the autoregulatory circuit comprising two different protein types, Antizymes (OAZ) and Antizyme Inhibitors (AZIN), which regulate the activity of the polyamine biosynthetic enzyme ornithine decarboxylase (ODC). While OAZ functions to decrease the intracellular polyamine levels by inhibiting ODC activity and exerting a negative control of polyamine uptake, AZIN operates to increase intracellular polyamine levels by binding and sequestering OAZ to relieve ODC inhibition and to increase polyamine uptake. Interestingly, OAZ and AZIN exhibit autoregulatory functions on polyamine independent pathways as well. A growing body of evidence demonstrates the dysregulation of AZIN expression in multiple cancers. Additionally, RNA editing of the Azin1 transcript results in a "gain-of-function" phenotype, which is shown to drive aggressive tumor types. This review will discuss the recent advances in AZIN's role in cancers via aberrant polyamine upregulation and its polyamine-independent protein regulation. This report will also highlight AZIN interaction with proteins outside the polyamine biosynthetic pathway and its potential implication to cancer pathogenesis. Finally, this review will reveal the protein interaction network of AZIN isoforms by analyzing three different interactome databases.

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Section: Physiological Role Of Antizyme Inhibitors Physiological Rolementioning

confidence: 99%

Role of Antizyme Inhibitor Proteins in Cancers and Beyond

Tulluri

Nemmara

2021

OTT

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“…Finally, emerging evidence has suggested that AZIN1 may have also functions not directly related with cell proliferation. For instance, the similarity in the expression pattern between AZIN1 and certain reproductive related genes in the hypothalamus, ovary and uterus during the rat oestrous cycle [73] or in the avian ovarian follicles [74], suggested a possible role of AZIN1 in reproductive physiology. Transcriptomic studies have also revealed that Azin1 is one of the genes that are consistently up-regulated by glucocorticoids in the brain [75].…”

Section: Antizyme Inhibitormentioning

confidence: 99%

Antizyme Inhibitors in Polyamine Metabolism and Beyond: Physiopathological Implications

2018

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The intracellular levels of polyamines, cationic molecules involved in a myriad of cellular functions ranging from cellular growth, differentiation and apoptosis, is precisely regulated by antizymes and antizyme inhibitors via the modulation of the polyamine biosynthetic and transport systems. Antizymes, which are mainly activated upon high polyamine levels, inhibit ornithine decarboxylase (ODC), the key enzyme of the polyamine biosynthetic route, and exert a negative control of polyamine intake. Antizyme inhibitors (AZINs), which are proteins highly homologous to ODC, selectively interact with antizymes, preventing their action on ODC and the polyamine transport system. In this review, we will update the recent advances on the structural, cellular and physiological functions of AZINs, with particular emphasis on the action of these proteins in the regulation of polyamine metabolism. In addition, we will describe emerging evidence that suggests that AZINs may also have polyamine-independent effects on cells. Finally, we will discuss how the dysregulation of AZIN activity has been implicated in certain human pathologies such as cancer, fibrosis or neurodegenerative diseases.

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“…Thus, OAZs are considered as negative regulators of intracellular polyamines. Polyamines are essential for cellular processes such as cell growth and proliferation, and hence play important roles in reproduction [4, 5]. Among four types of OAZs described to date [6], the prototype and the most highly investigated form is OAZ1, which is expressed ubiquitously at significantly higher levels and is believed to be the predominant factor in the regulation of ODC [7, 8].…”

Section: Introductionmentioning

confidence: 99%

OAZ1 knockdown enhances viability and inhibits ER and LHR transcriptions of granulosa cells in geese

Kang

Jiang

et al. 2017

PLoS ONE

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An increasing number of studies suggest that ornithine decarboxylase antizyme 1 (OAZ1), which is regarded as a tumor suppressor gene, regulates follicular development, ovulation, and steroidogenesis. The granulosa cells in the ovary play a critical role in these ovarian functions. However, the action of OAZ1 mediating physiological functions of granulosa cells is obscure. OAZ1 knockdown in granulosa cells of geese was carried out in the current study. The effect of OAZ1 knockdown on polyamine metabolism, cell proliferation, apoptosis, and hormone receptor transcription of primary granulosa cells in geese was measured. The viability of granulosa cells transfected with the shRNA OAZ1 at 48 h was significantly higher than the control (p<0.05). The level of putrescine and spermidine in granulosa cells down-regulating OAZ1 was 7.04- and 2.11- fold higher compared with the control, respectively (p<0.05). The CCND1, SMAD1, and BCL-2 mRNA expression levels in granulosa cells down-regulating OAZ1 were each significantly higher than the control, respectively (p<0.05), whereas the PCNA and CASPASE 3 expression levels were significantly lower than the control (p<0.05). The estradiol concentration, ER and LHR mRNA expression levels were significantly lower in granulosa cells down-regulating OAZ1 compared with the control (p<0.05). Taken together, our results indicated that OAZ1 knockdown elevated the putrescine and spermidine contents and enhanced granulosa cell viability and inhibited ER and LHR transcriptions of granulosa cells in geese.

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Molecular cloning and mRNA expression analysis of antizyme inhibitor 1 in the ovarian follicles of the Sichuan white goose

Cited by 7 publications

References 30 publications

Role of Antizyme Inhibitor Proteins in Cancers and Beyond

Role of Antizyme Inhibitor Proteins in Cancers and Beyond

Antizyme Inhibitors in Polyamine Metabolism and Beyond: Physiopathological Implications

OAZ1 knockdown enhances viability and inhibits ER and LHR transcriptions of granulosa cells in geese

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