Molecular Cloning and mRNA Expression of Geraniol-inducible Genes in Cultured Shoot Primordia of<i>Matricaria chamomilla</i>

Ashida, Yuki; Nishimoto, Masaki; Matsushima, Ayako; Watanabe, Junko; Hirata, Toshifumi

doi:10.1271/bbb.66.2511

Cited by 15 publications

(7 citation statements)

References 17 publications

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“…This study aimed to evaluate the expression levels of two genes WRKY1 and EREBP1 in lemon balm. These two genes are Geranial responsive elements which have been extracted from chamomile leaf primordial by Ashida et al ( 2002 ).…”

Section: Introductionmentioning

confidence: 99%

Investigating the effect of drought stress on expression of WRKY1 and EREBP1 genes and antioxidant enzyme activities in lemon balm (Melissa Officinalis L.)

et al. 2016

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Drought stress is a severe environmental constraint to plant productivity. In this study, gene expression of WRKY1 and EREBP1 genes and activity of two enzymes were investigated in two genotypes, G9 (as resistance) and G12 (as susceptible) of 10 days-old lemon balm seedlings. Experiments were done according to factorial design on the base of completely randomized design with three replications for expression analysis and enzyme assays. Seedlings were cultured in MS medium suspensions, including 0, 3, 6, 12 and 15 % w/v PEG 6000. Leaf samples were subsequently collected at 0, 3, 6, 24, 48, 72 h after culture. According to the results of the enzyme assay, SOD activity in resistant genotype was more than in sensitive one. POD activity was high in G9 in severe drought condition and in G12 this activity was increased at initial times of drought stress. The survey results revealed that the expression levels of both genes, EREBP1 and WRKY1, in G9 variety were more than G12, and in this respect we can say that the G9 variety is more resistant to drought.

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Section: Introductionmentioning

confidence: 99%

Investigating the effect of drought stress on expression of WRKY1 and EREBP1 genes and antioxidant enzyme activities in lemon balm (Melissa Officinalis L.)

et al. 2016

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“…As well, WRKY proteins have been implicated in the regulation of developmental processes such as trichome, 4 seed development, 5 embryogenesis, 6 leaf senescence, 7,8 dormancy, 9 plant growth 10 and metabolic pathways. 4,11 Since the first WRKY cDNA, SPF1 , was cloned from sweet potato ( Ipomoeabatatas ), 12 more and more WRKY genes in various plant species have been experimentally identified, including wild oat ( Avena fatua ; ABF1 ), 13 parsley ( Petroselinum crispum ; PcWRKY1, 2, 3 ), 14,15 Arabidopsis thaliana ( ZAP1 ), 16 orchardgrass ( Dactylis glomerata ), 17 tobacco ( Nicotiana tabacum ), 18–21 chamomile ( Matricaria chamomilla ), 22 rice ( Oryza sativa ), 23 sugarcane ( Saccharum hybrid cultivar), 24 bittersweet nightshade ( Solanum dulcamara ), 25 potato ( Solanum tuberosum ), 26,27 wheat ( Triticum aestivum ) 28 and grape ( Vitis vinifera ). 29 Zea mays (hereafter called maize) is not only a vital crop, but also serves as an important model system for basic biological research.…”

Section: Introductionmentioning

confidence: 99%

Molecular Phylogenetic and Expression Analysis of the Complete WRKY Transcription Factor Family in Maize

et al. 2012

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The WRKY transcription factors function in plant growth and development, and response to the biotic and abiotic stresses. Although many studies have focused on the functional identification of the WRKY transcription factors, much less is known about molecular phylogenetic and global expression analysis of the complete WRKY family in maize. In this study, we identified 136 WRKY proteins coded by 119 genes in the B73 inbred line from the complete genome and named them in an orderly manner. Then, a comprehensive phylogenetic analysis of five species was performed to explore the origin and evolutionary patterns of these WRKY genes, and the result showed that gene duplication is the major driving force for the origin of new groups and subgroups and functional divergence during evolution. Chromosomal location analysis of maize WRKY genes indicated that 20 gene clusters are distributed unevenly in the genome. Microarray-based expression analysis has revealed that 131 WRKY transcripts encoded by 116 genes may participate in the regulation of maize growth and development. Among them, 102 transcripts are stably expressed with a coefficient of variation (CV) value of <15%. The remaining 29 transcripts produced by 25 WRKY genes with the CV value of >15% are further analysed to discover new organ- or tissue-specific genes. In addition, microarray analyses of transcriptional responses to drought stress and fungal infection showed that maize WRKY proteins are involved in stress responses. All these results contribute to a deep probing into the roles of WRKY transcription factors in maize growth and development and stress tolerance.

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“…The first cDNA encoding a WRKY protein, SPF1, was cloned from sweet potato ( Ipomoea batatas ) [4]. Numerous genes for WRKY proteins have since been experimentally identified from more than 10 other plant species, including Arabidopsis thaliana [5,6], wild oats ( Avena fatua ) [7], orchardgrass ( Dactylis glomerata ) [8], barley ( Hordeum vulgare ) [9], tobacco ( Nicotiana tabacum ) [10-13], chamomile( Matricaria chamomilla ) [14], rice ( Oryza sativa ) [9,15], parsley ( Petroselinum crispum ) [16,17], a desert legume ( Retama raetam ) [18], sugarcane ( Saccharum hybrid cultivar) [19], bittersweet nightshade ( Solanum dulcamara ) [20], potato ( Solanum tuberosum ) [21,22], and wheat ( Triticum aestivum ) [9]. In addition, over 70 WRKY genes were identified in the Arabidopsis genome by sequence similarity comparisons [2,23].…”

Section: Introductionmentioning

confidence: 99%

The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants

2005

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BackgroundWRKY proteins are newly identified transcription factors involved in many plant processes including plant responses to biotic and abiotic stresses. To date, genes encoding WRKY proteins have been identified only from plants. Comprehensive search for WRKY genes in non-plant organisms and phylogenetic analysis would provide invaluable information about the origin and expansion of the WRKY family.ResultsWe searched all publicly available sequence data for WRKY genes. A single copy of the WRKY gene encoding two WRKY domains was identified from Giardia lamblia, a primitive eukaryote, Dictyostelium discoideum, a slime mold closely related to the lineage of animals and fungi, and the green alga Chlamydomonas reinhardtii, an early branching of plants. This ancestral WRKY gene seems to have duplicated many times during the evolution of plants, resulting in a large family in evolutionarily advanced flowering plants. In rice, the WRKY gene family consists of over 100 members. Analyses suggest that the C-terminal domain of the two-WRKY-domain encoding gene appears to be the ancestor of the single-WRKY-domain encoding genes, and that the WRKY domains may be phylogenetically classified into five groups. We propose a model to explain the WRKY family's origin in eukaryotes and expansion in plants.ConclusionsWRKY genes seem to have originated in early eukaryotes and greatly expanded in plants. The elucidation of the evolution and duplicative expansion of the WRKY genes should provide valuable information on their functions.

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Molecular Cloning and mRNA Expression of Geraniol-inducible Genes in Cultured Shoot Primordia ofMatricaria chamomilla

Cited by 15 publications

References 17 publications

Investigating the effect of drought stress on expression of WRKY1 and EREBP1 genes and antioxidant enzyme activities in lemon balm (Melissa Officinalis L.)

Investigating the effect of drought stress on expression of WRKY1 and EREBP1 genes and antioxidant enzyme activities in lemon balm (Melissa Officinalis L.)

Molecular Phylogenetic and Expression Analysis of the Complete WRKY Transcription Factor Family in Maize

The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants

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