Molecular cloning and nucleotide sequence of the genome of hog cholera virus

Meyers, Gregor; Rümenapf, Till; Thiel, Heinz-Jürgen

doi:10.1016/0042-6822(89)90625-9

Cited by 362 publications

(270 citation statements)

References 16 publications

Supporting

Mentioning

264

Contrasting

Unclassified

Order By: Relevance

“…CSFV specific primers were designed considering sequences of the 5'NTR of the genome of 55 different pestivirus strains, available in GenBank or in the database of CSF European reference laboratory of Hanover (Germany), that includes 37 CSFV representing subgroups described by Lowings [20]: 1.1, 1.2, 2.1, 2.2, 2.3 and disparate virus outgrouped [12,13,15,25,27,[31][32][33], fourteen BVDV belonging to types I and II [5,8,32] and four BDV isolates [4,29]. Sequences were aligned, using Clustal W software, and five oligonucleotides were primarily selected from highly conserved sequences among the different CSFV strains but showing high divergence with the other pestiviruses.…”

Section: Primers and Restriction Endonuclease Selectionmentioning

confidence: 99%

A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples

Agüero

Fernández²,

Romero

et al. 2004

Vet. Res.

View full text Add to dashboard Cite

-The development and standardisation of a novel, highly sensitive and specific one-step hot start multiplex RT-PCR assay is presented for the simultaneous and differential diagnosis of African swine fever (ASF) and Classical swine fever (CSF). The method uses two primer sets, each one specific for the corresponding virus, amplifying DNA fragments different in length, allowing a gel-based differential detection of the PCR products. Universal detection of ASF and CSF virus strains was achieved through selection of primers in conserved viral genome regions. The detection range was confirmed by analysis of a large collection of isolates of the two viruses. The high specificity of the assay was proven by testing related viruses, uninfected cell line cultures and healthy pig tissues. Additional confirmatory tests of the ASF and CSF virus amplicon specificity, based on restriction endonuclease analysis with BsmA I or Ban II, respectively, are also described. The analysis of whole blood and serum samples from experimentally infected animals proved the usefulness of the method for an early diagnosis of both diseases, even before the appearance of the first clinical signs. A study of 150 positive field samples from several ASF and CSF outbreaks showed the suitability of this method for a rapid (less than five hours), sensitive and specific differential diagnosis in clinical samples. In addition, a highly sensitive and specific uniplex RT-PCR for CSFV was also developed and standardised as a powerful tool for fast and early diagnosis of the disease.

show abstract

Section: Primers and Restriction Endonuclease Selectionmentioning

confidence: 99%

A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples

Agüero

Fernández²,

Romero

et al. 2004

Vet. Res.

View full text Add to dashboard Cite

show abstract

“…The viral positive single stranded genome of approximately 12.5 kb in size contains a single open reading frame (ORF) flanked by two non-translating terminal regions, named 5' and 3'-UTR. The single ORF is directly translated and gives rise to a long polyprotein which is co-translationally cleaved, originating 10 to 12 mature viral proteins (Collet et al 1988, Meyer et al 1989.…”

Section: Introduction Introduction Introduction Introduction Introducmentioning

confidence: 99%

Genetic characterization of Brazilian bovine viral diarrhea virus isolates by partial nucleotide sequencing of the 5'-UTR region

et al. 2006

View full text Add to dashboard Cite

As amostras foram agrupadas em BVDV-1 (11/19), BVDV-2 (6/19) e num terceiro grupo de amostras denominadas "atípicas" (2/ 19). Das onze amostras genotipadas como BVDV-1, oito amostras foram sub-genotipadas como BVDV-1a, enquanto que a maioria (4/6) das amostras de BVDV-2 foi agrupada como BVDV2b. Duas amostras provenientes de fetos bovinos abortados foram classificadas como atípicas, não BVDV-1 e 2. A presença da diversidade genética de BVDV detectada nas amostras estudadas pode ser responsável por falhas vacinais e de diagnóstico e deve influenciar nas estratégias de controle do BVDV aplicadas nas diferentes regiões brasileiras.TERMOS DE INDEXAÇÃO: BVDV-1, BVDV-2, Pestivirus, Brazil, análise filogenética. INTRODUCTION INTRODUCTION INTRODUCTION INTRODUCTION INTRODUCTIONInfections of cattle by bovine viral diarrhea virus (BVDV) are widespread cause of major economic losses to the cattle industry (Houe 1999). Clinical symptoms may involve the reproductive, respiratory, immune, and gastrointestinal systems, with signs that may range from disease with high mortality rates to asymptomatic infections. The latter is observed in most cases (Pellerin et al. 1994, Ridpath et al.1994, Baker 1995, Fray et al. 2000.BVDV is an enveloped RNA virus that belongs to family Flaviviridae, genus Pestivirus. The viral positive single stranded genome of approximately 12.5 kb in size contains a single open reading frame (ORF) flanked by two non-translating terminal regions, named 5' and 3'-UTR. The single ORF is directly translated and gives rise to a long polyprotein which is co-translationally cleaved, originating 10 to 12 mature viral proteins (Collet et al. 1988, Meyer et al. 1989.Isolates have been subdivided in genotypes BVDV-1 and BVDV-2. These were further split into subgenotypes.

show abstract

“…Two fragments, 431 and 190 bp, corresponding to 1269-1699 (E rns ) and 2517-2706 (E2) of the Alfort Tubingen sequence [20], respectively, were chosen for the phylogenetic analysis. For comparison, 27 reference strain sequences representing three groups and six subgroups were obtained from the GenBank and analyzed with Taiwanese CSFV isolates.…”

Section: Phylogenetic Treesmentioning

confidence: 99%

“…The causative agent of CSFV belongs to the genus Pestivirus within the family Flaviviridae [21]. The genome of CSFV consists of a positive-stranded RNA approximately 12.3 kb in length, and comprises a single large open reading frame (ORF) spanning 11,694 nucleotides that encode a polyprotein composed of 3,898 amino acids [20]. The polyprotein is proteolytically processed to produce both Phylogenetic analysis of CSFV in Taiwan method) from the Lasergene program package (DNASTAR, 2001).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of classical swine fever virus in Taiwan

et al. 2005

View full text Add to dashboard Cite

Summary. Two envelope glycoprotein (E rns and E2) regions of the classical swine fever virus (CSFV) were amplified by RT-PCR and sequenced directly from 158 specimens collected between 1989 and 2003 in Taiwan. Phylogenetic analysis of the two regions revealed a similar tree topology and the E rns region provided better discrimination than the E2 region. One hundred and fifteen isolates out of the 158 isolates were clustered within subgroup 2.1 (further classified as 2.1a and 2.1b) and 2.2, which were considered to be likely of the introduced strains, whereas the remaining 43 isolates were clustered within subgroup 3.4 and were considered to be of the endemic strains. The subgroup 2.1a viruses were first detected in 1994 and predominated from 1995 onwards. However, subgroup 3.4 viruses were prevalent in the early years, not being isolated after 1996. We have observed a dramatic switch in genotype from subgroup 3.4 to 2.1a. The subgroup 2.1a isolates are closely related to the Paderborn and Lao isolates, whereas 2.1b isolates have a close relationship to the Chinese Guangxi isolates. The phylogenetic tree of 27 CSFV sequences based on the complete envelope glycoprotein gene (E rns -E2) displayed better resolution than that based on the complete open reading frame.

show abstract

Molecular cloning and nucleotide sequence of the genome of hog cholera virus

Cited by 362 publications

References 16 publications

A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples

A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples

Genetic characterization of Brazilian bovine viral diarrhea virus isolates by partial nucleotide sequencing of the 5'-UTR region

Phylogenetic analysis of classical swine fever virus in Taiwan

Contact Info

Product

Resources

About