Molecular cloning and physical mapping of the genome of fish lymphocystis disease virus

Darai, Gholamreza; Delius, Hajo; Clarke, Jill; Apfel, Heiko; Schnitzler, Paul; Flügel, Rolf M.

doi:10.1016/0042-6822(85)90012-1

Cited by 68 publications

(27 citation statements)

References 17 publications

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“…LCDV-1, isolated in the United States, has been extensively studied, and its genome was characterized by molecular cloning and physical mapping about 20 years ago (5,6). The genome structure, found to be common to other iridoviruses, is circularly permuted and terminally redundant (5,6,28,37). In 1997, the LCDV-1 complete genomic DNA sequence was determined.…”

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confidence: 99%

Complete Genome Sequence of Lymphocystis Disease Virus Isolated from China

Zhang

Xiao

Xie

et al. 2004

J Virol

126

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Lymphocystis diseases in fish throughout the world have been extensively described. Here we report the complete genome sequence of lymphocystis disease virus isolated in China (LCDV-C), an LCDV isolated from cultured flounder (Paralichthys olivaceus) with lymphocystis disease in China. The LCDV-C genome is 186,250 bp, with a base composition of 27.25% G؉C. Computer-assisted analysis revealed 240 potential open reading frames (ORFs) and 176 nonoverlapping putative viral genes, which encode polypeptides ranging from 40 to 1,193 amino acids. The percent coding density is 67%, and the average length of each ORF is 702 bp. A search of the GenBank database using the 176 individual putative genes revealed 103 homologues to the corresponding ORFs of LCDV-1 and 73 potential genes that were not found in LCDV-1 and other iridoviruses. Among the 73 genes, there are 8 genes that contain conserved domains of cellular genes and 65 novel genes that do not show any significant homology with the sequences in public databases. Although a certain extent of similarity between putative gene products of LCDV-C and corresponding proteins of LCDV-1 was revealed, no colinearity was detected when their ORF arrangements and coding strategies were compared to each other, suggesting that a high degree of genetic rearrangements between them has occurred. And a large number of tandem and overlapping repeated sequences were observed in the LCDV-C genome. The deduced amino acid sequence of the major capsid protein (MCP) presents the highest identity to those of LCDV-1 and other iridoviruses among the LCDV-C gene products. Furthermore, a phylogenetic tree was constructed based on the multiple alignments of nine MCP amino acid sequences. Interestingly, LCDV-C and LCDV-1 were clustered together, but their amino acid identity is much less than that in other clusters. The unexpected levels of divergence between their genomes in size, gene organization, and gene product identity suggest that LCDV-C and LCDV-1 shouldn't belong to a same species and that LCDV-C should be considered a species different from LCDV-1.

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confidence: 99%

Complete Genome Sequence of Lymphocystis Disease Virus Isolated from China

Zhang

Xiao

Xie

et al. 2004

J Virol

126

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“…Regions of nonhomology between these two viruses have been described, accounting for 12-5 % of the IV9 genome and 40 % of the IV16 genome. Between these regions there are large sections containing repeat sequences, as reported for other IVs (Goorha & Murti, 1982;Darai et al, 1985;Tajbakhsh et al, 1986;Schnitzler et al, 1987;Fischer et al, 1990), although the precise nature of these sequences has not been described for IV9. The PCR product of IV10 was only partially cleaved by XhoI.…”

Section: Discussionmentioning

confidence: 81%

“…FV3 and FLCDV share no nucleic acid homology, and their relationships with other members of the Iridoviridae are unclear (Willis, 1990). The genomes of FV3 and FLCDV are highly methylated in contrast to the structure of the invertebrate IV genomes that have been studied (Darai et al, 1983(Darai et al, , 1985Essani, 1990).…”

Section: Introductionmentioning

confidence: 99%

Proposals for a new classification of iridescent viruses

Williams¹,

Cory²

1994

Journal of General Virology

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“…Iridoviruses are icosahedral cytoplasmic DNA viruses that infect invertebrates and poikilothermic vertebrates, including insects, fish, amphibians, and reptiles (64,73). The viral genomes are both circularly permuted and terminally redundant, which are unique features of eukaryotic virus genomes (10,11,12,65). Additionally, the iridoviruses that infect vertebrates have highly methylated genomes (10,74).…”

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confidence: 99%

Infectious Spleen and Kidney Necrosis Virus ORF48R Functions as a New Viral Vascular Endothelial Growth Factor

Wang

et al. 2008

J Virol

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Molecular cloning and physical mapping of the genome of fish lymphocystis disease virus

Cited by 68 publications

References 17 publications

Complete Genome Sequence of Lymphocystis Disease Virus Isolated from China

Complete Genome Sequence of Lymphocystis Disease Virus Isolated from China

Proposals for a new classification of iridescent viruses

Infectious Spleen and Kidney Necrosis Virus ORF48R Functions as a New Viral Vascular Endothelial Growth Factor

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