Molecular cloning and sequence analysis of a mitogen-activated protein kinase gene in the Antarctic yeast Rhodotorula mucilaginosa AN5

Abstract

The mitogen-activated protein kinase (MAPK) cascades play important roles in various signaling transduction networks of biotic and abiotic stress responses. However, MAPK signaling pathways in cold-active yeast Rhodotorula mucilaginosa have not been reported comprehensively. In the present study, MAPK gene (RmMAPK) was first cloned and characterized from Antarctic sea ice yeast R. mucilaginosa AN5. The full length of the RmMAPK gene is 1086 bp and encodes a 361 amino acids protein with a predicted molecular mass of 40.9 kDa and a pI of 5.25. The RmMAPK contains 11 MAPK conserved subdomains and the phosphorylation motif TGY located in the activation loop of the kinase. Quantitative real-time PCR assay revealed that the expression of RmMAPK up-regulated rapidly and significantly when yeast cells were subjected to low temperature (4 °C ), high salinity (120‰ NaCl) and heavy metal (2 mmol/L CuCl2), which suggested that the RmMAPK might act as a key function in response to extreme stresses, such as low temperature, high salinity and heavy metal.

Pyrimethamine inhibits cell growth by inducing cell senescence and boosting CD8+ T-cell mediated cytotoxicity in colorectal cancer

“…MAPK pathway plays an essential role in cancer development. ERKs, JNKs, and p38MAPKs are the three prominent MAPK family members in mammalian cells, among which p38MAPK is well known to be closely linked to cellular senescence [33][34][35][36]. Surprisingly, the MAPK signaling pathway was one of the most enriched pathways in our RNA-seq results.…”