Molecular cloning and sequence of Sparus aurata skeletal myosin light chains expressed in white muscle: developmental expression and thyroid regulation

Moutou, Katerina A.; Power, Deborah M.; Canário, Adelino V.M.

doi:10.1016/s1095-6433(99)90269-6

Cited by 18 publications

(38 citation statements)

References 50 publications

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“…For example, the MLC1, MLC2 and MLC3 clones showed a degree of similarity with zebrafish (Danio rerio, Hamilton) of 83, 92 and 91%, respectively. Overall, herein MLC expression was restricted to the Fish Physiol Biochem (2010) 36:1125-11321127 fast muscle, which agrees with data obtained in juvenile and developing gilthead seabream (Moutou et al 2001). In common with the gilthead seabream, a notable change in the tissue expression pattern of the various forms of MLC occurs during blackspot seabream development.…”

Section: Resultssupporting

confidence: 85%

“…The high degree of similarity among the MLC clones isolated from the gilthead seabream (Moutou et al 2001) with the ones previously isolated from other fish allow us to conclude that the MLC expression obtained in blackspot seabream is specific. For example, the MLC1, MLC2 and MLC3 clones showed a degree of similarity with zebrafish (Danio rerio, Hamilton) of 83, 92 and 91%, respectively.…”

Section: Resultsmentioning

confidence: 59%

“…Linearised minipreps of pGEM-T Easy (Promega) plasmid vector containing inserts of the complete coding region of MLC1, MLC2 and MLC3, isolated from gilthead seabream (Sparus aurata, Linnaeus) were used as templates (Moutou et al 2001). The linearised vector was purified and an in vitro transcription carried out using 20U of T7 RNA polymerase in transcription buffer (Amersham Biosciences) with 1 ll of digoxigenin-RNA labelling mix (Roche Diagnostics, Mannheim, Germany), for 1.5 h at 37°C.…”

Section: Probe Generationmentioning

confidence: 99%

“…Various biochemical, histochemical and immunohistochemical investigations have revealed that during fish muscle development there is a sequential expression of a range of myofibrillar protein isoforms (e.g. Carpenè and Veggetti 1981;Scapolo et al 1988;Martinez et al 1991;Brooks and Johnston 1993;Crockford and Johnston 1993;Veggetti et al 1993;Johnston 1994;Johnston and Horne 1994;Johnston et al 1998;Huriaux et al 1999Huriaux et al , 2003Focant et al 2000;Moutou et al 2001;Chauvigné et al 2006). In the plaice Pleuronectes platessa (Linneaus), larval MLC2 is gradually replaced by adult MLC2 in postmetamorphic fish, whereas changes in myosin heavy-chain composition occurs later (Brooks and Johnston 1993).…”

Section: Introductionmentioning

confidence: 99%

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Expression of the myosin light chains 1, 2 and 3 in the muscle of blackspot seabream (Pagellus bogaraveo, Brunnich), during development

Silva

Power

Valente

et al. 2010

Fish Physiol Biochem

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Previous studies on the histochemistry and immunoreactivity of fibres in lateral muscle of blackspot seabream indicated that there is a developmental transition in the composition of myofibrillar proteins, which presumably reflects changes in contractile function as the fish grows. We hypothesize that the phenomenon underscores age and spatial differences in the expression of myosin light chains (MLC), not studied yet in this species. In this study, we examined selected stages in the post-hatching development of the muscle of blackspot seabream: hatching (0 days), mouth opening (5 days), weaning (40 days) and juveniles (70 days). The spatial expression of embryonic MLC 1 (MLC1), 2 (MLC2) and 3 (MLC3) was studied by in situ hybridization. Overall, MLC expression patterns were overlapping and restricted to the fast muscle. At hatching and mouth opening, all MLC types were highly expressed throughout the musculature in fast muscle. The expression levels in fast muscle remained high until weaning when germinal zones appeared on the dorsal and ventral areas. The germinal zones were characterized by small-diameter fast fibres with high levels of MLC expression. This pattern persisted up to day 70, when the germinal zones disappeared and expression of MLCs was observed only in the smaller cells of the fast muscle mosaic. These results support our hypothesis and, together with previous imunoand histochemistry results, allow a better understanding of the mechanism of muscle differentiation and growth in fish beyond larval stages, and form-the basis for further comparative and experimental studies with this economically relevant species.

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Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 59%

Section: Probe Generationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Expression of the myosin light chains 1, 2 and 3 in the muscle of blackspot seabream (Pagellus bogaraveo, Brunnich), during development

Silva

Power

Valente

et al. 2010

Fish Physiol Biochem

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show abstract

“…This result is consistent with the earlier reports that the Ca 2+ -binding domain is the most conserved feature in EMLCs. However, there is a controversial that whether any EMLC binds to Ca 2+ or not [27][28][29]. In this study we demonstrated that recombinant MLC1 and MLC3, when expressed in and purified from E. coli, displayed altered electrophoretic mobility in the presence of Ca 2+ which is a common property in EF-hand family members [30].…”

Section: Discussionmentioning

confidence: 72%

Expression and functional analyses of two essential myosin light chains from the fast white muscle of the spotted mandarin fish Siniperca scherzeri

Chu¹,

Li²,

Wang³

et al. 2013

Turk J Bioch

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Myosin regulatory light chain expression in trout muscle

et al. 2007

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Muscle's contractile properties can vary along different trajectories, including between muscle fiber types, along the body (within a muscle fiber type), and between developmental stages. This study explores the role of the regulatory myosin light chain (MLC2) in modulating contractile properties in rainbow trout myotomal muscle. Rainbow trout show longitudinal variations in muscle activation and relaxation, with faster contractile properties in the anterior myotome. The expression of two muscle proteins, troponin T and parvalbumin, vary along the length of trout in concert with shifts in muscle activation and relaxation. However, there is no longitudinal variation in myosin heavy chain in trout. This study explores the role of MLC2 (or regulatory light chain), part of the myosin hexamer, in contributing to longitudinal variations in contractile properties of trout swimming muscle. We cloned and sequenced two isoforms of MLC2 from trout muscle and used real-time quantitative polymerase chain reaction to assess the relative expression of these two isoforms in red and white muscle from different body positions of two ages of rainbow trout: parr and smolt. Longitudinal variations in slow (sMLC2) but not fast (fMLC2) regulatory light chain isoforms were observed in young trout parr but not older trout smolts. The differences in sMLC2 expression correlated with shifts in muscle contractile properties in the parr.

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Molecular cloning and sequence of Sparus aurata skeletal myosin light chains expressed in white muscle: developmental expression and thyroid regulation

Cited by 18 publications

References 50 publications

Expression of the myosin light chains 1, 2 and 3 in the muscle of blackspot seabream (Pagellus bogaraveo, Brunnich), during development

Expression of the myosin light chains 1, 2 and 3 in the muscle of blackspot seabream (Pagellus bogaraveo, Brunnich), during development

Expression and functional analyses of two essential myosin light chains from the fast white muscle of the spotted mandarin fish Siniperca scherzeri

Myosin regulatory light chain expression in trout muscle

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