Molecular Cloning and Sexually Dimorphic Expression of Two Dmrt Genes in the Giant Freshwater Prawn, Macrobrachium rosenbergii

Male Sexual Development and the Androgenic Gland: Novel Insights through the de novo Assembled Transcriptome of the Eastern Spiny Lobster,<b><i> Sagmariasus verreauxi</i></b>

“…The ancient emergence and conserved function of the Dmrts is evident in their characterization amongst the non-bilaterian animals (such as corals and placozoa), suggesting that they emerged before the divergence of the Eumetazoans [Picard et al, 2015]. Even though the Dmrts have been broadly described [Wexler et al, 2014;Picard et al, 2015], their documentation amongst the Decapods is still limited [Zhang and Qiu, 2010;Ma et al, 2012;Yu et al, 2014].…”

Potential involvement of a DMRT family member ( Mr‐Dsx ) in the regulation of sexual differentiation and moulting in the giant river prawn Macrobrachium rosenbergii

“…While these genes have been widely studied in insects, nematodes and vertebrates (Hong et al, ; Vinas et al, ; Zarkower, ), information on the molecular characteristics and functions of Dmrt genes in crustaceans is still limited (Yang et al, ; Yu et al, , ). To date, members of the Dmrt gene family have been identified from several crustaceans, including the water flea D. magna (Toyota et al, ), the Chinese mitten crab Eriocheir sinensis (Zhang & Qiu, ), the Chinese shrimp F. chinensis (Li et al, ), the amphipod H. azteca (Rotllant et al, ), the eastern rock lobster S. verreauxi (Chandler, Fitzgibbon, Smith, Elizur, & Ventura, ) and the giant freshwater prawn M. rosenbergii (Yu et al, ). In particular, two Dmrt genes (namely Dmrt11E and Dmrt99B ) have been characterized in M. rosenbergii .…”

“…In particular, two Dmrt genes (namely Dmrt11E and Dmrt99B ) have been characterized in M. rosenbergii . These two genes were reportedly involved in the sexual differentiation and embryonic development of M. rosenbergii , and Dmrt11E can influence the development of male gonad by modulating the expression of IAG (Yu et al, ). In this study, we identified a new Dmrt gene ( Mr‐Dsx ) by molecular cloning techniques and bioinformatics analysis based on previous transcriptome sequencing data.…”

“…In particular, the position of Dmrt family genes in the signalling pathways orchestrating sex differentiation of M. rosenbergii largely remains elusive, and the regulatory network between eyestalk neuropeptides, IAG and Dmrt genes is still one of the most puzzling questions that need to be resolved (Bai et al, ; Li et al, ). To date, only two Dmrt genes have been identified from M. rosenbergii , namely Dmrt11E and Dmrt99B (Yu et al, ). These two genes were reportedly implicated in the embryonic development and sexual differentiation of M. rosenbergii , and Dmrt11E has been further validated to affect the development of male gonads by regulating the expression of IAG (Yu et al, ).…”

“…To date, only two Dmrt genes have been identified from M. rosenbergii , namely Dmrt11E and Dmrt99B (Yu et al, ). These two genes were reportedly implicated in the embryonic development and sexual differentiation of M. rosenbergii , and Dmrt11E has been further validated to affect the development of male gonads by regulating the expression of IAG (Yu et al, ). Except these two genes, no other Dmrt homologues have been reported for M. rosenbergii .…”