Molecular cloning and tissue expression of porcine β‐defensin‐1

Zhang, Guolong; Wu, Hua; Shi, Jishu; Ganz, Tomas; Ross, Christopher R.; Blecha, Frank

doi:10.1016/s0014-5793(98)00134-3

Cited by 83 publications

(64 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Obviously, this is not the case. The expression of pBD-1 correlates well with the pattern described before (Zhang et al, 1998). For pBD-2 no expression sites have been described, but based on these studies, high levels of pBD-2 mRNA are found in liver, kidney and to a lesser extend aorta.…”

Section: Discussionsupporting

confidence: 83%

Expression of β-defensins pBD-1 and pBD-2 along the small intestinal tract of the pig: Lack of upregulation in vivo upon Salmonella typhimurium infection

Veldhuizen

Dijk

Tersteeg

et al. 2007

Molecular Immunology

View full text Add to dashboard Cite

Defensins are antimicrobial peptides that play an important role in the innate immune response in the intestine. Up to date, only one ␤-defensin (pBD-1), has been described in pig, which was found to be expressed at low levels in the intestine. We set-up a quantitative PCR method to detect the gene expression of pBD-1 and a newly discovered porcine ␤-defensin, pBD-2. Expression of pBD-1 mRNA increased from the proximal to the distal part of the intestine whereas pBD-2 expression decreased. The main gene expression sites for pBD-2 were kidney and liver, whereas pBD-1 was mainly expressed in tongue. The porcine small intestinal segment perfusion (SISP) technique was used to investigate effects of Salmonella typhimurium DT104 on intestinal morphology and pBD-1 and pBD-2 mRNA levels in vivo. The early responses were studied 2, 4 and 8 h postinfection in four separate jejunal and ileal segments. Immunohistochemistry showed invasion of the mucosa by Salmonella and changes in intestinal morphology. However, no concomitant changes in expression of either pBD-1 or pBD-2 were observed. We conclude that at least two defensins are differentially expressed in the intestine of pigs, and that expression of both defensins is not altered by S. typhimurium under these conditions.

show abstract

Section: Discussionsupporting

confidence: 83%

Expression of β-defensins pBD-1 and pBD-2 along the small intestinal tract of the pig: Lack of upregulation in vivo upon Salmonella typhimurium infection

Veldhuizen

Dijk

Tersteeg

et al. 2007

Molecular Immunology

View full text Add to dashboard Cite

show abstract

“…A novel AMP, termed NK-lysin, was isolated from porcine small intestine and has been shown to be a new effector molecule of cytotoxic T and NK cells [5]. Recently, a porcine β-defensin, pBD-1 was cloned and found to be expressed throughout epithelia of the respiratory and gastrointestinal tracts [134]. All of these AMPs, except cecropin P1 and NK-lysin, belong to either the defensin or the cathelicidin family, which are the two major groups of AMPs found in most mammalian species [37][38][39]66].…”

Section: Arsenal Of Porcine Ampsmentioning

confidence: 99%

“…Recently, we cloned the full-length cDNA for pBD-1, which is the only member of the defensin family identified in pigs thus far [134]. The pBD-1 mRNA is expressed abundantly in tongue epithelia and to a lesser extent throughout the respiratory and digestive tracts.…”

Section: Pbd-1 a Porcine Form Of Epithelial β-Defensinmentioning

confidence: 99%

Porcine antimicrobial peptides: New prospects for ancient molecules of host defense

Zhang¹,

Ross²,

Blecha³

2000

Vet. Res.

Self Cite

110

View full text Add to dashboard Cite

-Antimicrobial peptides (AMPs) are small, endogenous, polycationic molecules that constitute a ubiquitous and significant component of innate immunity. These natural antibiotics have broad microbicidal activity against various bacteria, fungi, and enveloped viruses. Because most AMPs kill bacteria by physical disruption of cell membranes, which may prevent microorganisms from developing resistance against these agents, they are being explored as possible alternatives to conventional antibiotics. Pigs, like many other mammals, produce an impressive array of AMPs, which are synthesized predominantly by host leukocytic phagocytes or mucosal epithelial cells. Currently, more than a dozen distinct porcine AMPs have been identified and a majority belongs to the cathelicidin family. This review briefly summarizes recent advances in porcine AMP research with an emphasis on the diverse biological functions of each peptide. Mechanisms of action of these AMPs and their role in the resistance to infections are considered. Finally, the current status of pharmaceutical and agricultural uses of AMPs as well as future prospects for their application in the food animal industry is discussed.pig / antimicrobial peptide / cathelicidin / defensin / innate immunity Résumé -Peptides antimicrobiens porcins : nouvelles perspectives pour d'anciennes molécules de défense de l'hôte. Les peptides antimicrobiens sont de petites molécules endogènes, polycationiques, qui représentent un élément important et ubiquitaire des défenses immunes naturelles. Ces antibiotiques naturels ont un effet antimicrobien à large spectre, à la fois contre des bactéries, des moisissures et des virus enveloppés. Comme la plupart de ces peptides antimicrobiens tuent les bactéries par altération physique de leur membrane, ce qui peut éviter le développement de souches microbiennes résistantes à ces agents, ils représentent des alternatives possibles à l'emploi des antibiotiques classiques. Le porc, comme de nombreux autres mammifères, produit une gamme importante de peptides antimicrobiens, synthétisés pour l'essentiel par les leucocytes à activité phagocytaire, ou par les cellules épithéliales des muqueuses. Il y a actuellement plus d'une douzaine de peptides antimicrobiens identifiés chez le porc, dont la majorité fait partie de la famille des cathélicidines. Cet article résume les progrès récents accomplis dans le domaine des peptides antimicrobiens du porc, Vet. Res. 31 (2000) 277-296 277

show abstract

“…The only member of the defensin family identified in pigs thus far is the porcine β-defensin [77]. Although α-defensins are the most abundant anti-microbial peptides in granules of polymorphonuclear cells or intestinal Paneth cells in many mammalian species [79], they have not been found in pigs.…”

Section: Role Of Iec In the Secretion Of Defensins And Other Antibactmentioning

confidence: 99%

Role of intestinal epithelial cells in the innate immune defence of the pig intestine

Oswald

2006

Vet. Res.

139

View full text Add to dashboard Cite

-The intestinal epithelium serves as a dynamic barrier, which in the course of its normal function, maintains regulated uptake of nutrients and water while excluding potential pathogens. Over the past decade many studies have also revealed the immunological importance of intestinal epithelial cells (IEC). IEC have developed a variety of mechanisms to reduce the risk of infection by invasive pathogens or damage by toxic compounds. The effective maintenance of a physical barrier function is dependent on the establishment of well-organised intercellular junctions and a constant state of regeneration/renewal of the epithelium. IEC also participate in the innate immune responsiveness of the intestine by their ability to secrete mucus and antimicrobial peptides. IEC are also able to secrete cytokines and to respond to exogenous chemokines. This review summarises the current knowledge of the innate immune mechanisms developed by porcine IEC.

show abstract

Molecular cloning and tissue expression of porcine β‐defensin‐1

Cited by 83 publications

References 19 publications

Expression of β-defensins pBD-1 and pBD-2 along the small intestinal tract of the pig: Lack of upregulation in vivo upon Salmonella typhimurium infection

Expression of β-defensins pBD-1 and pBD-2 along the small intestinal tract of the pig: Lack of upregulation in vivo upon Salmonella typhimurium infection

Porcine antimicrobial peptides: New prospects for ancient molecules of host defense

Role of intestinal epithelial cells in the innate immune defence of the pig intestine

Contact Info

Product

Resources

About