Molecular cloning, characterization and expression analysis of ubiquitin protein ligase gene (As-ubpl) from Artemia sinica

Li, Zengrong; Yao, Feng; Chen, Yifei; Zhang, Rui; Lv, Ying; Zhao, Ning; Wang, Ting; Xin, Wenting; Lin, Hui; Zou, Xiangyang

doi:10.1016/j.cbpb.2013.03.009

Cited by 6 publications

(5 citation statements)

References 41 publications

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“…A. sinica has a relatively low classification status, and few differences were found between As-RBBP4 and homologs in other species. Other genes of A. sinica also showed similar characteristics as it is classified between higher vertebrates and low invertebrates Jiang et al, 2007;Li et al, 2013;Wang et al, 2013;Li et al, 2014 ).…”

Section: Discussionmentioning

confidence: 69%

Cloning and expression of retinoblastoma-binding protein 4 gene in embryo diapause termination and in response to salinity stress from brine shrimp Artemia sinica

Wang

Yao

Liang

et al. 2016

Gene

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Section: Discussionmentioning

confidence: 69%

Cloning and expression of retinoblastoma-binding protein 4 gene in embryo diapause termination and in response to salinity stress from brine shrimp Artemia sinica

Wang

Yao

Liang

et al. 2016

Gene

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“…A. sinica cysts were brought from Yuncheng, Shanxi, China (Li et al, ). The diapause cysts were stored at −20°C for at least one month to terminate diapause.…”

Section: Methodsmentioning

confidence: 99%

The potential role of Annexin 3 in diapause embryo restart of Artemia sinica and in response to stress of low temperature

Yao

Huang

et al. 2019

Molecular Reproduction Devel

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Annexins are highly conserved and ubiquitous in various somatic cell types. They are involved in membrane transport and a range of calcium-regulated activities on the cell membrane surface, including vesicular transport, membrane fusion in exocytosis, signal transduction, and formation of calcium channels. They also regulate inflammatory response, cell differentiation, and interaction between cytoskeletal proteins. In this study, for the first time, an ANX3 gene from Artemia sinica (As-anx3) was cloned. The As-anx3 full-length complementary DNA comprises 1,024 bp and has a 948 bp open reading frame encoding a 315-amino-acid polypeptide with four ANX domains. The profiles of both As-ANX3 mRNA and protein expression exhibited peaks at the 0 hr stage and had the same significant downregulation trend throughout the post-diapause embryo development stage. The ERK1/2, the phosphorylation levels of ERK1/2, and cell cycle-related protein (CDK4) expressions were analyzed by western blot analysis. The results showed that CDK4 presented a significantly ascending trend from 0 and 40 hr, although the phosphorylation levels of ERK1/2 did not increase significantly. The transcriptional and protein expressions of As-ANX3 were highly upregulated when the temperature was lowered from 25 to 15°C, but the expressions showed a gradual downward trend when the temperature was further lowered to 5°C. These results indicated that As-ANX3 plays a crucial role in restarting diapause and low-temperature stress in A. sinica. K E Y W O R D Sannexin 3, Artemia sinica, diapause embryo development

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“…A. sinica cysts were collected from Yuncheng salt lake (Shanxi, China)56, and kept at a low temperature to terminate diapause. The cysts were hatched in filtered seawater, at 28 °C, with a salinity level of 28‰ and a light intensity of 1000Lux, according to a previously described method57.…”

Section: Methodsmentioning

confidence: 99%

“…Real-time PCR was performed in triplicate for every sample in a parallel design, using the SYBR Premix Ex Taq (Takara) and the Takara detection system, according to the manufacturer’s protocol. PCR reaction conditions according to the previously described method5657. The A. sinica β- actin gene was used as a normalization control for each starting quantity of RNA.…”

Section: Methodsmentioning

confidence: 99%

APC/CCDC20 and APC/C play pivotal roles in the process of embryonic development in Artemia sinica

Zhang

Yao

Luan

et al. 2016

Sci Rep

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Anaphase Promoting Complex or Cyclosome (APC/C) is a representative E3 ubiquitin ligase, triggering the transition of metaphase to anaphase by regulating degradation and ensures the exit from mitosis. Cell division cycle 20 (CDC20) and Cell division cycle 20 related protein 1 (CDH1), as co-activators of APC/C, play significant roles in the spindle assembly checkpoint, guiding ubiquitin-mediated degradation, together with CDC23. During the embryonic development of the brine shrimp, Artemia sinica, CDC20, CDH1 and CDC23 participate in cell cycle regulation, but the specific mechanisms of their activities remain unknown. Herein, the full-length cDNAs of cdc20 and cdc23 from A. sinica were cloned. Real-time PCR analyzed the expression levels of As-cdc20 and As-cdc23. The locations of CDH1, CDC20 and CDC23 showed no tissue or organ specificity. Furthermore, western blotting showed that the levels of As-CDC20, securin, cyclin B, CDK1, CDH1, CDC14B, CDC23 and geminin proteins conformed to their complicated degradation relationships during different embryo stages. Our research revealed that As-CDC20, As-CDH1 and APC mediate the mitotic progression, downstream proteins degradation and cellular differentiation in the process of embryonic development in A. sinica.

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Molecular cloning, characterization and expression analysis of ubiquitin protein ligase gene (As-ubpl) from Artemia sinica

Cited by 6 publications

References 41 publications

Cloning and expression of retinoblastoma-binding protein 4 gene in embryo diapause termination and in response to salinity stress from brine shrimp Artemia sinica

Cloning and expression of retinoblastoma-binding protein 4 gene in embryo diapause termination and in response to salinity stress from brine shrimp Artemia sinica

The potential role of Annexin 3 in diapause embryo restart of Artemia sinica and in response to stress of low temperature

APC/CCDC20 and APC/C play pivotal roles in the process of embryonic development in Artemia sinica

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