Molecular cloning, characterization, and expression analyses of immunoglobulin tau heavy chain (IgT) in largemouth bass (Micropterus salmoides)

Yang, Shun Fa; Ming, Yue; Ma, Yuanxin; Zhang, Xintang; Qian, Shichao; Fei, Hui

doi:10.1016/j.aqrep.2021.100989

Cited by 3 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Characterization of IgT heavy chain in most teleost have found an arrangement of four CH (CH1, CH2, CH3, CH4) domains apart from species such as the fugu and stickleback that possesses lesser CH numbers in non-consecutive order ( 20 , 21 ). In the case of the ASB, the heavy chain of IgT was shown to retain the prototypical arrangement and number observed previously in perch-like fish ( 14 , 22 , 23 ). Additionally, amino acid sequence comparisons of ASB sIgT revealed the highest conservation to sIgT of the gilthead sea bream (GSB) ( Sparus aurata ), a fellow member of the Perciformes order.…”

Section: Discussionsupporting

confidence: 65%

“…A study of immersion NNV challenge and immune responses of seven-band grouper has found early virus replication in the gills of infected fish prior to dissemination to the brain ( 29 ). Stimulation of MALTs through immersion challenge was found to mostly enhance localized production of antigen-specific mucosal IgT with rare induction of systemic IgT ( 14 , 15 , 23 , 30 ).…”

Section: Discussionmentioning

confidence: 99%

“…Analysis of sIgT expression in tissues of GSB and European sea bass has likewise found a trend for higher IgT expression in the gill, intestine and head kidney and lower IgT transcription in the spleen ( 14 , 22 ). In the Nile tilapia, the highest IgT expression was found in the spleen ( 23 ), while flounder and turbot exhibited high IgT expression in the gill, spleen, liver, head kidney and trunk kidney ( 15 , 28 ). Taken together, the results suggested a tissue specificity for basal sIgT expression with variation in expression levels between teleost species.…”

Section: Discussionmentioning

confidence: 99%

“…Taken together, the results suggested a tissue specificity for basal sIgT expression with variation in expression levels between teleost species. Exposure of teleost to infectious agents or immunizing antigen was demonstrated to modulate IgT expression through stimulation of the adaptive mucosal immunity ( 14 , 22 , 23 ). European sea bass infected with nodavirus exhibited increased IgT transcription in the gill and spleen post-infection ( 22 ).…”

Section: Discussionmentioning

confidence: 99%

“…European sea bass infected with nodavirus exhibited increased IgT transcription in the gill and spleen post-infection ( 22 ). In contrast, immersion vaccination of largemouth bass induced significantly higher IgT mRNA levels in mucosal tissues such as the gill and intestine ( 23 ). In the current study, RGNNV immersion was found to elicit a higher response of IgT expression in mucosal tissues of ASB with the strongest response observed in the MALTs ie., gill, skin and intestine.…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Identification and characterization of immunoglobulin tau (IgT) in Asian Seabass (Lates calcarifer) and mucosal immune response to nervous necrosis virus

et al. 2023

View full text Add to dashboard Cite

Mucosal immunity plays a critical role in the protection of teleost fish against infection, but mucosal immunoglobulin of important aquaculture species unique to Southeast Asia remained greatly understudied. In this study, the sequence of immunoglobulin T (IgT) from Asian sea bass (ASB) is described for the first time. IgT of ASB possesses the characteristic structure of immunoglobulin with a variable heavy chain and four CH4 domains. The CH2-CH4 domains and full-length IgT were expressed and CH2-CH4 specific antibody was validated against full-length IgT expressed in Sf9 III cells. Subsequent use of the anti-CH2-CH4 antibody in immunofluorescence staining confirmed the presence of IgT-positive cells in the ASB gill and intestine. The constitutive expression of ASB IgT was characterized in different tissues and in response to red-spotted grouper nervous necrosis virus (RGNNV) infection. The highest basal expression of secretory IgT (sIgT) was observed in the mucosal and lymphoid tissues such as the gills, intestine and head kidney. Following NNV infection, IgT expression was upregulated in the head kidney and mucosal tissues. Moreover, a significant increase in localized IgT was found in gills and intestines of infected fish on day 14 post-infection. Interestingly, a significant increase in NNV-specific IgT secretion was only observed in the gills of the infected group. Our results suggest that ASB IgT may play an important role in the adaptive mucosal immune responses against viral infection and could potentially be adapted as a tool for the evaluation of prospective mucosal vaccines and adjuvants for the species.

show abstract

Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Identification and characterization of immunoglobulin tau (IgT) in Asian Seabass (Lates calcarifer) and mucosal immune response to nervous necrosis virus

et al. 2023

View full text Add to dashboard Cite

show abstract

Oral Vaccination With Recombinant Pichia pastoris Expressing Iridovirus Major Capsid Protein Elicits Protective Immunity in Largemouth Bass (Micropterus salmoides)

et al. 2022

View full text Add to dashboard Cite

Largemouth bass iridovirus (LMBV) can cause high mortality and lead to heavy economic loss in the cultivation of largemouth bass, but there was no effective treatment. Here, the present study constructed a recombinant Pichia pastoris expressing LMBV major capsid protein (MCPD). The recombinant GS115-pW317-MCPD was then used to immunize largemouth bass via oral administration, and mucosal immune response mediated by immunoglobulins (Igs) was measured after oral immunization. Serum antibody levels were measured by ELISA, neutralizing antibody titers were determined by serum neutralization test (SNT), antigen presentation-related gene expressions were detected by RT-PCR, and the histopathological characteristics of immunized fish were assessed after challenging with 0.1 ml 107.19 TCID50/ml LMBV. The relative percentage survival (RPS) was also determined. Our results showed that the serum antibody titers of immunized fish were significantly higher than that of control groups (P < 0.05). IgT and IgM expressions in gut were increased significantly after vaccination with GS115-pW317-MCPD; however, much stronger response in gut was observed as compared with gill. The expression levels of major histocompatibility complex (MHC) II, CD8, and T-cell receptor (TCR) were significantly elevated in GS115-pW317-MCPD group (P < 0.05), while CD4 and MHC I transcription levels remained unchanged after oral immunization (P > 0.05). The RPS of fish orally immunized with 1.0 × 108 CFU/g GS115-pW317-MCPD was reached up to 41.6% after challenge with 0.1 ml 109.46 TCID50/ml LMBV. Moreover, orally immunizing with GS115-pW317-MCPD can relieve the pathological damage caused by LMBV. Therefore, GS115-pW317-MCPD showed a promising potential against LMBV.

show abstract

Dietary Supplementation of Chlorella vulgaris Effectively Enhanced the Intestinal Antioxidant Capacity and Immune Status of Micropterus salmoides

Huang

et al. 2023

Antioxidants

View full text Add to dashboard Cite

An M. salmoides fish meal diet was supplemented with 0 (CHL0, Control), 38 (CHL38), 76 (CHL76), 114 (CHL114), and 152 (CHL152) mg/kg C. vulgaris for 60 days, and their serum and intestinal samples were analyzed. The results showed that the albumin (ALB) and total protein (TP) contents were observably enhanced in the CHL76 group compared with the Control group. The intestinal glutathione (GSH) and glutathione peroxidase (GSH-Px) contents were enhanced significantly in the CHL76 group, while the total antioxidant capacity (T-AOC) was enhanced in the CHL38 group, compared with the Control group. However, supplementation of >76 g/kg C. vulgaris significantly inhibited the superoxide dismutase (SOD) activity in the intestines of M. salmoides. Moreover, the malondialdehyde (MDA) content was observably dropped in the CHL-supplemented groups compared with the Control group. Transcriptome analysis of the CHL76 and Control groups displayed a total of 1384 differentially expressed genes (DEGs). KEGG analysis revealed that these DEGs were enriched in apoptosis, cytokine–cytokine receptor interaction, tight junction (TJ), and phagosome signaling pathways, which were associated with improved intestinal immunity in the CHL76 group. Additionally, the DEGs enriched in the above pathways were also correlated with the antioxidant parameters, such as catalase (CAT), GSH, GSH-Px, SOD, T-AOC, and MDA. Therefore, our study found that dietary supplementation of C. vulgaris effectively enhanced the intestinal antioxidant capacity of M. salmoides by increasing antioxidant enzyme activity and decreasing MDA content. Additionally, dietary supplementation of C. vulgaris improved the intestinal immune status of M. salmoides by reducing proapoptotic and proinflammatory factors, increasing intestinal TJs- and phagosome-related genes expressions, and increasing the serum ALB and TP contents. Lastly, quadratic regression analysis of the serum biochemical indices (ALB and TP) and intestinal antioxidant parameters (GSH-Px and GSH) revealed that the optimal supplemental level of C. vulgaris in the M. salmoides diet was 58.25–77.7 g/kg.

show abstract

Molecular cloning, characterization, and expression analyses of immunoglobulin tau heavy chain (IgT) in largemouth bass (Micropterus salmoides)

Cited by 3 publications

References 20 publications

Identification and characterization of immunoglobulin tau (IgT) in Asian Seabass (Lates calcarifer) and mucosal immune response to nervous necrosis virus

Identification and characterization of immunoglobulin tau (IgT) in Asian Seabass (Lates calcarifer) and mucosal immune response to nervous necrosis virus

Oral Vaccination With Recombinant Pichia pastoris Expressing Iridovirus Major Capsid Protein Elicits Protective Immunity in Largemouth Bass (Micropterus salmoides)

Dietary Supplementation of Chlorella vulgaris Effectively Enhanced the Intestinal Antioxidant Capacity and Immune Status of Micropterus salmoides

Contact Info

Product

Resources

About