Molecular cloning, characterization and expression patterns of HSP60 in the grass carp (Ctenopharyngodon idella)

Cloning and differential expression of five heat shock protein genes associated with thermal stress and development in the polyphagous predatory mite Neoseiulus cucumeris (Acari: Phytoseiidae)

“…The phylogenetic tree analysis of Hsp60 (Fig. S4) implied that Hsp60 genes are highly conserved throughout evolution, which is consistent with the observations on various mitochondrial Hsp60s (Abdallah et al 2000;Wong et al 2004;Xu and Qin 2012;Xu et al 2011). The NcHsp40 sequence contained a conserved J domain and a CR domain (Fig.…”

“…In addition, the predicted amino acid sequence of NcHsp60 contained two conserved ATP/Mg 2? -binding motifs that have also been observed in Portunus trituberculatus (Xu and Qin 2012) and in Ctenopharyngodon idella (Xu et al 2011). These results suggest that Hsp60 genes may be involved in similar substrate-refolding processes coupled with ATP hydrolysis.…”

Matrix metalloproteinase 2 of grass carp Ctenopharyngodon idella (CiMMP2) is involved in the immune response against bacterial infection

“…Sense (Table 1) [28]. The threshold cycle (CT) value was determined using the 7500 v2.0 Software, and the data were exported into Microsoft Excel for subsequent analysis, where the relative expression ratios of target genes in the treated groups versus that in the control group were calculated by the 2 ÀDDCT method [33].…”

“…Immune responses in fish can be experimentally stimulated by injection of bacteria [27]. Therefore, an immunological challenge with formalin-killed A. hydrophila has great potential to understand immune regulatory effects [28].…”

Molecular cloning of heat shock protein 60 from Marsupenaeus japonicus and its expression profiles at early developmental stages and response to heat stress

“…HSPs are categorized into several families, including HSP110, Hsp100, HSP 90, HSP70, HSP 60 and the small HSPs (sHSPs), according to their sequence homology and molecular mass (Feder & Hofmann, ). Among the heat shock protein family, HSP60 plays an important role in the protein‐folding system and is capable of responding to various stressors (Chaurasia et al., ; Shi et al., ; Xu & Qin, ; Xu et al., ; Yang et al., ; Zhou, Wang, Xin, et al., ). Several HSP60 from shrimps were recently identified and characterized (Shi et al., ; Zhou, Wang, He, et al., ).…”