Molecular cloning, characterization, and expression of two TNFRs from the pearl oyster Pinctada fucata martensii

Wu, Yuyuan; He, Jun-Jun; Yao, Gaoyou; Liang, Haiying; Huang, Xuemin

doi:10.1016/j.fsi.2020.01.010

Cited by 13 publications

(8 citation statements)

References 43 publications

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“…Several reports have indicated that the TNF-TNFR system (TNFR, TRAF, and NF-kB family members) responded to the nuclear insertion surgery. These genes may be involved in allograft immunity in pearl oysters (Wu et al, 2020). Here, in hepatopancreas tissues, PmTRAF3 and PmTRAF4 transcripts with significant up-regulation at different times after nuclear insertion surgery, and PmTRAF2 and PmTRAF6 with downregulation at all times, indicating that four TRAFs might participate in the immune defense at different time.…”

Section: Discussionmentioning

confidence: 74%

“…Consistent with the above results, the mRNA transcripts of PmTRAF2 and PmTRAF4 were highly expressed in hepatopancreas and gills, indicating that they operated in immune defense. TRAF2 is one of the major recruitment targets for TRADD and TNFR5 proteins, and all three genes from P. f. martensii share high expression in gills and hepatopancreas (Hsu et al, 1996;He et al, 2019a;Wu et al, 2020). It can be speculated that PmTRADD and PmTNFR5 can recruit PmTRAF2 and play a role in apoptosis and other immune responses.…”

Section: Discussionmentioning

confidence: 99%

“…Sixty oysters were used to perform nucleus insertion surgery by an experienced technician at Xuwen Breeding Base, China. In nucleus insertion surgery, a piece of mantle graft from a donor oyster with a spherical shell bead or nucleus is transplanted into the gonad of the host oyster (Wu et al, 2020). At 0, 12, 24, 48, and 72 h after insertion, hepatopancreas samples were collected with 10 pearl oysters.…”

Section: Experimental Samplementioning

confidence: 99%

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Molecular cloning and function of two tumor necrosis factor receptor-associated factors genes (TRAF2 and TRAF4) from Pinctada fucata martensii

et al. 2023

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Tumor necrosis factor receptor-associated factors (TRAFs) have been studied in a few mollusks and participate in various biological processes, like apoptosis, immune response, stress, and inflammatory response. However, TRAFs’ function and mechanism of pearl oysters (Pinctada fucata martensii) are still unclear. In this study, we identified novel PmTRAF2 and PmTRAF4 from P. f. martensii. The deduced PmTRAF2 and PmTRAF4 proteins contain TRAF-type zinc finger domain and MATH domain, while PmTRAF4 lacks a RING finger domain. Multiple sequence alignment revealed that PmTRAF2 and PmTRAF4 had high homology with the ortholog of other species. Phylogenic analysis indicated that PmTRAF4 was closer to the homolog protein of Mytilus edulis and Mytilus galloprovincialis, and PmTRAF2 was closer to Crassostrea gigas TRAF2. The real-time quantitative PCR (qPCR) analysis revealed that PmTRAF2 and PmTRAF4 were expressed in all six tissues, and both of them were significantly expressed in hepatopancreas and gill (p < 0.01). Under lipopolysaccharide (LPS) stimulation, polyinosinic acid (PolyI:C) stimulation, and nucleus insertion surgery, the transcripts of PmTRAF2, PmTRAF3, PmTRAF4 and PmTRAF6 in hepatopancreas were markedly changed at corresponding time points. These results have indicated that these genes may play a role in P. f. martensii innate immunity. Yeast two-hybrid assays show that PmTRAF2 interacts with PmTRAF6 but not PmTRAF3, potentially affecting downstream immune signaling pathways. Our findings provide new perspectives for further investigation of TRAFs’ immune mechanisms in bivalves.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Experimental Samplementioning

confidence: 99%

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Molecular cloning and function of two tumor necrosis factor receptor-associated factors genes (TRAF2 and TRAF4) from Pinctada fucata martensii

et al. 2023

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“…RNA interference has evolved into a powerful tool for probing the functions of genes ( Hannon, 2002 ), which has successfully led to the silence of homologous genes ( Fire et al, 1998 ). Studies have shown that RNAi inhibition of PmTNFR1 and PmTNFR5 downregulated the downstream genes, which suggested that they mediated the NF-κB signaling pathway and were closely related to immune defense in the pearl oyster ( Wu et al, 2020 ). Similarly, the silencing experiment of LvGrx 2 expression indicated that it was involved in the regulation of oxidative defense and antioxidant system in shrimp under ammonia stress ( Zheng et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Vital Role of Glutamate Dehydrogenase Gene in Ammonia Detoxification and the Association Between its SNPs and Ammonia Tolerance in Sinonovacula constricta

et al. 2021

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Increasing evidence has revealed accumulated ammonia will cause adverse effects on the growth, reproduction, and survival of aquatic animals. As a marine benthic mollusk, the razor clam Sinonovacula constricta shows better growth and survival under high ammonia nitrogen environment. However, little is known about its adaptation mechanisms to high ammonia stress in an integrated mariculture system. In this study, we analyzed the association between the polymorphism of glutamate dehydrogenase gene (GDH), a key gene involved in ammonia nitrogen detoxification, and ammonia tolerance. The results showed that 26 and 22 single-nucleotide polymorphisms (SNPs) of GDH in S. constricta (denoted as Sc-GDH) were identified from two geographical populations, respectively. Among them, two SNPs (c.323T > C and c.620C > T) exhibited a significant and strong association with ammonia tolerance, suggesting that Sc-GDH gene could serve as a potential genetic marker for molecular marker–assisted selection to increase survival rate and production of S. constricta. To observe the histological morphology and explore the histocellular localization of Sc-GDH, by paraffin section and hematoxylin–eosin staining, the gills were divided into gill filament (contains columnar and flattened cells) and gill cilia, whereas hepatopancreas was made up of individual hepatocytes. The results of immunohistochemistry indicated that the columnar cells of gill filaments and the endothelial cells of hepatocytes were the major sites for Sc-GDH secretion. Under ammonia stress (180 mg/L), the expression levels of Sc-GDH were extremely significantly downregulated at 24, 48, 72, and 96 h (P < 0.01) after RNA interference. Thus, we can speculate that Sc-GDH gene may play an important role in the defense process against ammonia stress. Overall, these findings laid a foundation for further research on the adaptive mechanisms to ammonia–nitrogen tolerance for S. constricta.

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“…Proteins in the tumor necrosis factor receptor (TNFR) superfamily have significant roles in inflammation, apoptosis, autoimmunity, and organogenesis. Two TNFR gene homologs (PmTNFR1 and PmTNFR5) were characterized from P. f. martensii, and Wu et al (2020) found RNAi inhibition of PmTNFR1 and PmTNFR5 gene expression. Results suggested that the NF-κB signaling pathway may be activated by PmTNFR1 and PmTNFR5 and that these genes are closely related to allograft immunity in P. f. martensii.…”

Section: Immune Responsementioning

confidence: 99%

Research Trends of Development on Pearl Bivalve Mollusks Based on a Bibliometric Network Analysis in the Past 25 Years

Cheng

Bai

2021

Front. Mar. Sci.

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Freshwater pearl mussels and marine pearl oysters are major producers of cultured pearls. With the development of pearl farming, pearl bivalve mollusks have attracted significant research attention over the past 25 years. To provide an overview of this research, we conducted a bibliometric analysis of publications from the Web of Science Core Collection database from 1995 to 2020. A network map generated by VOSviewer software was used to evaluate studies of pearl bivalve mollusks in terms of author, country, organization, journal, and keywords. A total of 2,288 publications were obtained, showing an annual increase in the number of papers. Researchers based in China were a major contributor to the field and there was active cooperation among authors. Research focused on mussel and oyster developmental biology, growth, culture mode, molecular biology, and ecological conservation. In recent years, hot topics, such as growth performance and traits, pearl formation and biomineralization, and immune response, have been widely discussed. Genomics, transcriptomics, proteomics, and metabonomics analyses were commonly used to analyze the regulatory mechanism of coding and non-coding genes. The interaction between the environment and farming activities showed the importance of sustainable development. Interdisciplinary research could solve some of the issues facing the pearl bivalve mollusk farming. In conclusion, our findings could serve as another way to understand research trends in pearl bivalve mollusks and contribute to future studies.

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Molecular cloning, characterization, and expression of two TNFRs from the pearl oyster Pinctada fucata martensii

Cited by 13 publications

References 43 publications

Molecular cloning and function of two tumor necrosis factor receptor-associated factors genes (TRAF2 and TRAF4) from Pinctada fucata martensii

Molecular cloning and function of two tumor necrosis factor receptor-associated factors genes (TRAF2 and TRAF4) from Pinctada fucata martensii

Vital Role of Glutamate Dehydrogenase Gene in Ammonia Detoxification and the Association Between its SNPs and Ammonia Tolerance in Sinonovacula constricta

Research Trends of Development on Pearl Bivalve Mollusks Based on a Bibliometric Network Analysis in the Past 25 Years

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