Molecular cloning, characterization and expression profiles of Annexin family (ANXA1~A6) in yellow catfish (Pelteobagrus fulvidraco) and ANX regulation by CpG ODN responding to bacterial infection

Cai, Hao; Wei, Jiatian; Shen, Haokun; Li, Jiayi; Fan, Qin; Zhao, Zengjue; Deng, Jinbo; Ming, Feiping; Zeng, Min; Ma, Miaopeng; Zhao, Peijing; Liang, Qiao‐Mei; Jia, Junhao; Zhang, Shuxia; Zhang, Linghua

doi:10.1016/j.fsi.2020.02.032

Cited by 6 publications

(2 citation statements)

References 66 publications

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“…The yellow catfish is rich in nutrients, with a particularly delicious flavour, and has a high economic value. This freshwater fish is popular with people of East and South Asia (Cai et al ., 2020; Wang et al ., 2016; Zhou et al ., 2015). Nonetheless, with the increase in the culture density of yellow catfish, outbreaks of microbial diseases in the breeding process have become more frequent, which is detrimental to the development of yellow catfish culture.…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide identification of the traf gene family in yellow catfish (Pelteobagrus fulvidraco) and analysis of their expression in response to bacterial challenge

Nie

Wang

Zhang

et al. 2022

Journal of Fish Biology

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Tumour necrosis factor (TNF) receptor‐associated factor (TRAF) is a receptor protein that has important functions in the immune system. Nonetheless, there have been few reports of traf genes in teleost fishes. The present study aimed to identify the traf genes from the genomic information of yellow catfish (Pelteobagrus fulvidraco). Eight traf genes were identified and named, which are distributed on different chromosomes but have similar conserved protein domains. Phylogenetic and syntenic analyses demonstrated conservation of traf genes during evolution. In addition, yellow catfish has the relatively rare traf1 and traf5 genes. Gene structure and motif analysis revealed the homology and distribution diversity of the traf genes. Quantitative real‐time reverse transcription PCR was used to study the expression patterns of traf genes in healthy fish tissues and after infection by Aeromonas hydrophila. The results demonstrated significant changes in traf gene expression, indicating a potential role in innate immunity.

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Section: Introductionmentioning

confidence: 99%

Genome‐wide identification of the traf gene family in yellow catfish (Pelteobagrus fulvidraco) and analysis of their expression in response to bacterial challenge

Nie

Wang

Zhang

et al. 2022

Journal of Fish Biology

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“…Strikingly, synthetic oligonucleotides containing unmethylated CpG motifs (CpG-ODNs), which are recognized by Toll-like receptor (TLR) 9 in mammals while by TLR21 in avian and teleost, can precisely meet the demands [ 2 , 3 , 4 , 5 ]. Accumulating evidence showed that CpG-ODNs are excellent modulators for innate immune response in protecting hosts of different species from infections [ 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ]. Meanwhile, applications of CpG-ODNs as vaccine adjuvants (acquired immunoenhancers) in COVID-19 and Hepatitis B vaccines demonstrated their undeniable safety [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

LACpG10-HL Functions Effectively in Antibiotic-Free and Healthy Husbandry by Improving the Innate Immunity

Pan

Zhao

et al. 2022

IJMS

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Antibiotics are broadly restricted in modern husbandry farming, necessitating the need for efficient and low-cost immunomodulatory preparations in antibiotic-free and healthful farming. As is known to all, CpG oligonucleotides (CpG-ODNs, an effective innate immunostimulatory agent) recognized by TLR9 in mammals (while TLR21 in avians) could collaborate with some united agent to induce stronger immune responses, but the cost is prohibitively expensive for farmers. Here, considering the coordination between TLR2 and TLR9/TLR21, we firstly proposed the idea that the well-fermented Lactococcus lactis could be utilized as a CpG-plasmid carrier (LACpG10) to enhance the host's innate immunity against pathogenic invasion. In the present study, after obtaining LACpG10-HL from homogenized and lyophilized recombinant strain LACpG10, we treated primary chicken lymphocytes, two cell lines (HD11 and IPEC-J2), and chickens with LACpG10-HL, CpG plasmids (pNZ8148-CpG10), and other stimulants, and respectively confirmed the effects by conducting qRT-PCR, bacterial infection assays, and a zoological experiment. Our data showed that LACpG10-HL could induce excellent innate immunity by regulating autophagy reactions, cytokine expression, and motivating PRRs. Interestingly, despite having no direct antiseptic effect, LACpG10-HL improved the antibacterial capacities of lymphocytes and enterocytes at the first line of defense. Most importantly, water-supplied LACpG10-HL treatment reduced the average adverse event rates, demonstrating that LACpG10-HL maintained its excellent immunostimulatory and protective properties under farming conditions. Our research not only contributes to revealing the satisfactory effects of LACpG10-HL but also sheds new light on a cost-effective solution with optimal immune effects in green, antibiotic-free, and healthful husbandry farming.

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Characterization and expression profiles of cGAS (cyclic GMP-AMP synthase) and STING (stimulator of interferon) genes in various immune tissues of hybrid yellow catfish under bacterial infections

Guo,

Zeng,

Wang

et al. 2024

Aquaculture Reports

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Molecular cloning, characterization and expression profiles of Annexin family (ANXA1~A6) in yellow catfish (Pelteobagrus fulvidraco) and ANX regulation by CpG ODN responding to bacterial infection

Cited by 6 publications

References 66 publications

Genome‐wide identification of the traf gene family in yellow catfish (Pelteobagrus fulvidraco) and analysis of their expression in response to bacterial challenge

Genome‐wide identification of the traf gene family in yellow catfish (Pelteobagrus fulvidraco) and analysis of their expression in response to bacterial challenge

LACpG10-HL Functions Effectively in Antibiotic-Free and Healthy Husbandry by Improving the Innate Immunity

Characterization and expression profiles of cGAS (cyclic GMP-AMP synthase) and STING (stimulator of interferon) genes in various immune tissues of hybrid yellow catfish under bacterial infections

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