Molecular Cloning, Characterization and Expression of the Phenylalanine Ammonia-Lyase Gene from Juglans regia

Xu, Feng; Deng, Guang; Cheng, Shuiyuan; Zhang, Weiwei; Huang, Xiaohua; Li, Linling; Cheng, Hua; Rong, Xiaofeng; Li, Jinbao

doi:10.3390/molecules17077810

Cited by 81 publications

(84 citation statements)

References 50 publications

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“…Some highly conserved residues were found in the , Q 497 and 3, 5-dihydro-5-methylidene-4H-imidazol-4-one (MIO), which were considered to be of significant importance for substrate binding, catalytic activity and LMIO formation (Xu et al, 2008). The mechanism was in agreement with the recent report from Petroselinum crispum research (Röther et al, 2002).…”

Section: Cytologic Characterization and Function Domain Investigationsupporting

confidence: 80%

Isolation and characterization of the anthocyanidin genes pal, f3h and dfr of Scutellaria viscidula (Lamiaceae)

Wei

Yao²,

Kang³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Anthocyanidin is a group of flavonoid compounds used as a vegetable pigment and plays an important role in flower coloration and environmental adaptations of the Chinese ornamental plant Scutellaria viscidula. We determined the cDNA sequences of phenylalanine ammonia-lyase (SvPAL), flavanone 3-hydroxylase (SvF3H) and dihydroflavonol 4-reductase (SvDFR) genes in S. viscidula. Comparative analysis showed that the protein products of these three genes did not have a transit peptide at their N-terminal portion, which indicated that these enzymes were directly involved in the substrate conversion in the cytoplasmic matrix. Bioinformatic analysis further revealed that Svpal, Svf3h and Svdfr were the members of flavonoid biosynthetic genes with highly conserved motifs. Based on phylogenetic tree analysis, it appears that PAL, F3H or DFR from ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 10 (4): 3385-3402 (2011) W. Lei et al. 3386 different plants might have originated from the same ancestor. This study can help to map and regulate the important stages involved in anthocyanidin biosynthesis by genetic engineering to diversify flower color and improve the ornamental value of S. viscidula.

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Section: Cytologic Characterization and Function Domain Investigationsupporting

confidence: 80%

Isolation and characterization of the anthocyanidin genes pal, f3h and dfr of Scutellaria viscidula (Lamiaceae)

Wei

Yao²,

Kang³

et al. 2011

Genet. Mol. Res.

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“…This gene was previously characterized and designated as JrPAL (Xu et al 2012a). Expression of the transcription factor gene JrCBF was also studied .…”

Section: Progress On Transcriptomicsmentioning

confidence: 99%

Walnut: past and future of genetic improvement

Bernard

Lheureux²,

Dirlewanger

2017

Tree Genetics & Genomes

163

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Persian or English walnut (Juglans regia L.), the walnut species cultivated for nut production, is one of the oldest food sources known. Persian walnuts, native to the mountain valleys of Central Asia, are grown worldwide in temperate areas. World production exceeds three million tons since 2012, mostly provided by China, the USA, and Iran. Despite very ancient culture of walnut species (Juglans spp.), breeding actually started in the twentieth century. Using a range of methodologies, from morphological markers to the most recent advances in genome analysis, many genetic studies of walnut have been conducted during the past 30 years, including examination of diversity, determination of relationships within or among germplasm collections and populations, phylogenetic and origin elucidation, genetic map construction, and biotic or abiotic stress investigations. The genetic improvement of walnut has undergone great evolution. The producing countries of the Middle East have widely studied morphological characteristics of walnut. The USA and France, for example, are behind important cultivar releases such as BChandler^and BFranquette.^Finally, genomics represents a major breakthrough in walnut improvement, in particular by recent sequencing of both chloroplast and nuclear genomes. This review summarizes worldwide molecular and Bomics^studies and gives an overview of the main walnut breeding programs.

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“…CCC treatments also significantly induced the activities of PAL, CHS, and CHI in a concentration-dependent manner in ginkgo leaves. Recent findings demonstrated that PAL, CHS and CHI were both key regulatory enzymes in flavonoid biosynthesis in G. biloba leaves Xu et al, 2007Xu et al, , 2008 and observed that CCC could induce the transcription of chalcone isomerase gene (GbCHI) in ginkgo leaves . The transcript levels of several genes encod-…”

Section: Discussionmentioning

confidence: 99%

Effect of Chlorocholine Chloride on Chlorophyll, Photosynthesis, Soluble Sugar and Flavonoids of Ginkgo biloba

Zhang

Cheng

et al. 2013

Not Bot Hort Agrobot Cluj

Self Cite

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The flavonoids content determines the quality characteristics of Ginkgo biloba extract that could be increased by using of plant growth regulators. The objective of study was to investigate the effect of chlorocholine chloride (CCC), an anti-gibberellin growth retardant, on photosynthesis, chlorophyll, soluble sugar, total amino acids and phenylalanine contents, flavonoid accumulation, and flavonoids enzyme activity in G. biloba leaves. The ginkgo seedlings were grown in the greenhouse conditions with foliar applications of 0 (control), 0.5, 1.0 and 2.0 g l -1 CCC. Results showed that 0.5, 1.0 and 2.0 g l -1 CCC treatments significantly increased photosynthetic rates of leaves, the contents of chlorophyll, soluble sugar, total amino acids and phenylalnine in ginkgo leaves. Total polyphenols, flavonoids, anthocyanins content, phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS) and chalcone isomerase (CHI) activities were all significantly increased by 1.0 and 2.0 g l -1 CCC treatments. Foliar treatment with CCC therefore might be a useful means of improving pharmacological properties of G. biloba leaves.

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Molecular Cloning, Characterization and Expression of the Phenylalanine Ammonia-Lyase Gene from Juglans regia

Cited by 81 publications

References 50 publications

Isolation and characterization of the anthocyanidin genes pal, f3h and dfr of Scutellaria viscidula (Lamiaceae)

Isolation and characterization of the anthocyanidin genes pal, f3h and dfr of Scutellaria viscidula (Lamiaceae)

Walnut: past and future of genetic improvement

Effect of Chlorocholine Chloride on Chlorophyll, Photosynthesis, Soluble Sugar and Flavonoids of Ginkgo biloba

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