Molecular cloning, expression analysis of the IgT gene and detection of IgT+ B cells in the half-smooth tongue sole (Cynoglossus semilaevis)

Wang, Lei; Zhang, Fumiao; Chen, Yadong; Wang, Shuangyan; Chen, Zhangfan; Zhou, Qian; Chen, Songlin

doi:10.1016/j.fsi.2022.02.051

Cited by 7 publications

(3 citation statements)

References 48 publications

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“…Te highest expression levels of pl-IgM and pl-pIgR were observed both in the mucosal-associated tissues and systemic immune tissues, including the intestine, spleen, kidney, gill, liver, and skin. Te present result is consistent with the previous studies on Japanese founder, turbot, and half-smooth tongue sole [13,24,30]. Te expression levels of IgM and pIgR were afected in fsh under infection with bacteria, parasites, and viruses.…”

Section: Discussionsupporting

confidence: 93%

“…SC is free or bound to polymeric IgM (or IgA) and contributes as a microbial scavenger to protect the epithelial surface [19,20]. Previous studies reported the gene sequences of pIgR in fugu (Takifugu rubripes) [21], common carp [17], orange-spotted grouper (Epinephelus coioides) [22], tlantic salmon (Salmo salar) [23], olive founder [24], turbot [25], sea bass (Lateolabrax japonicus) [26], crucian carp (Carassius auratus) [27], dojo loach (Misgurnus anguillicaudatus) [28], grass carp (Ctenopharyngodon idellus) [29], half-smooth tongue sole (Cynoglossus semilaevis) [30], and largemouth bass (Micropterus salmoides) [31]. Previously written articles demonstrated that the pIgR transported tetrameric IgM into the skin mucus in fugu and oliver founder [18,21].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of Immunoglobulin M (IgM) and Polymeric Immunoglobulin Receptor (pIgR) and Expression Response to Vibrio harveyi Challenge in Leopard Coral Grouper (Plectropomus leopardus)

Wang

Liu

Zhang

et al. 2023

Aquaculture Research

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Immunoglobulin M (IgM) is the primary antibody in fish, which is transported from epithelial cells into the external secretion system by polymeric immunoglobulin receptor (pIgR). In this study, the full length of IgM and pIgR complementary DNA sequences from leopard coral grouper (Plectropomus leopardus) was characterized, and their expression levels under Vibrio harveyi infection were studied. cDNA sequence analysis showed that the pl-IgM heavy chain cDNA contained an open reading frame (ORF) of 1797 bp encoding a polypeptide of 597 amino acids consisting of a signal peptide, a variable region, and 4 constant regions. pl-pIgR (1041 bp) encodes 346 amino acids with 2 Ig-like domains (ILDs). The expression levels of pl-IgM and pl-pIgR in healthy fish were mainly in the intestine and spleen of fish, which indicates the relationship between the mucosal-associated lymphoid tissue and system lymphoid tissue. Messenger RNA (mRNA) expression profiles of pl-IgM and pl-pIgR were significantly increased in the intestine, head kidney, spleen, and liver after V. harveyi infection. The cDNA sequences of two ILDs were cloned and expressed in Escherichia coli BL21 (DE3). The recombinant pl-pIgR protein had antibacterial function against V. harveyi and Vibrio alginolyticus at 200 ng/μl. Our results indicate that pl-IgM and pl-pIgR are involved in the mucosal-associated immune and systemic immune response in the antibacterial immunity of leopard coral grouper.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Immunoglobulin M (IgM) and Polymeric Immunoglobulin Receptor (pIgR) and Expression Response to Vibrio harveyi Challenge in Leopard Coral Grouper (Plectropomus leopardus)

Wang

Liu

Zhang

et al. 2023

Aquaculture Research

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“…Detection of IgT expression in teleost mucosal tissues has previously been established through either immunofluorescence staining or in-situ hybridization ( 7 , 22 , 24 , 26 , 27 ). Previous use of immunofluorescence staining has identified IgT + B cells in the epithelium of gill filaments and within the intestinal epithelium and lamina propria of the intestine in multiple teleost species ( 22 , 24 , 26 ). In this study, IgT-expressing cells were similarly located in the lamina propria of the intestine and primary lamellae (PL) of the gills of the ASB.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of immunoglobulin tau (IgT) in Asian Seabass (Lates calcarifer) and mucosal immune response to nervous necrosis virus

et al. 2023

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Mucosal immunity plays a critical role in the protection of teleost fish against infection, but mucosal immunoglobulin of important aquaculture species unique to Southeast Asia remained greatly understudied. In this study, the sequence of immunoglobulin T (IgT) from Asian sea bass (ASB) is described for the first time. IgT of ASB possesses the characteristic structure of immunoglobulin with a variable heavy chain and four CH4 domains. The CH2-CH4 domains and full-length IgT were expressed and CH2-CH4 specific antibody was validated against full-length IgT expressed in Sf9 III cells. Subsequent use of the anti-CH2-CH4 antibody in immunofluorescence staining confirmed the presence of IgT-positive cells in the ASB gill and intestine. The constitutive expression of ASB IgT was characterized in different tissues and in response to red-spotted grouper nervous necrosis virus (RGNNV) infection. The highest basal expression of secretory IgT (sIgT) was observed in the mucosal and lymphoid tissues such as the gills, intestine and head kidney. Following NNV infection, IgT expression was upregulated in the head kidney and mucosal tissues. Moreover, a significant increase in localized IgT was found in gills and intestines of infected fish on day 14 post-infection. Interestingly, a significant increase in NNV-specific IgT secretion was only observed in the gills of the infected group. Our results suggest that ASB IgT may play an important role in the adaptive mucosal immune responses against viral infection and could potentially be adapted as a tool for the evaluation of prospective mucosal vaccines and adjuvants for the species.

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Characterization of a membrane Fcγ receptor in largemouth bass (Micropterus saloumoides) and its response to bacterial challenge

Wang,

Wu,

et al. 2024

Fish Physiol Biochem

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Molecular cloning, expression analysis of the IgT gene and detection of IgT+ B cells in the half-smooth tongue sole (Cynoglossus semilaevis)

Cited by 7 publications

References 48 publications

Molecular Characterization of Immunoglobulin M (IgM) and Polymeric Immunoglobulin Receptor (pIgR) and Expression Response to Vibrio harveyi Challenge in Leopard Coral Grouper (Plectropomus leopardus)

Molecular Characterization of Immunoglobulin M (IgM) and Polymeric Immunoglobulin Receptor (pIgR) and Expression Response to Vibrio harveyi Challenge in Leopard Coral Grouper (Plectropomus leopardus)

Identification and characterization of immunoglobulin tau (IgT) in Asian Seabass (Lates calcarifer) and mucosal immune response to nervous necrosis virus

Characterization of a membrane Fcγ receptor in largemouth bass (Micropterus saloumoides) and its response to bacterial challenge

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