Molecular cloning, expression, and characterization of BmSOD3 in silkworm (Bombyx mori)

Abstract

Superoxide dismutases (SODs) play an essential role in eliminating excess reactive oxygen species and maintaining the redox balance of the immune system. To study the function of BmSOD3 in silkworm, 543-bp full-length complementary DNA-encoding BmSOD3 was cloned from silkworm. The BmSOD3 amino acids were compared to their homologs, and several highly conserved regions were analyzed. We also carried out phylogenetic analyses of the SOD gene. Our results showed that the BmSOD3 gene belonged with the ecCu/Zn SOD gene. The BmSOD3 gene was transformed into the pET28a vector for functional expression in Escherichia coli. The sodium saltpolyacrylamide gel electrophoresis results showed that the molecular weight of recombinant BmSOD3 was about 22 kDa. The recombinant protein BmSOD3 was purified to detect its properties. After purification analyses, the enzyme activity showed Cu/Zn SOD activity, and the specific activity of the purified enzyme was 0.51 U/mg. The BmSOD3 transcripts showed tissue-specific expression in the midgut and malpighian tubule. The immune microarray data for BmSOD3 showed an expression signal that had a strong response to the induction of four pathogens (Bacillus bombyseptieus, Beauveria bassiana, E. coli, and nuclear polyhedrosis virus), particularly after infection for 24 h, which indicates that the BmSOD3 gene plays a key role in

Transcriptome of the pygmy grasshopper Formosatettix qinlingensis (Orthoptera: Tetrigidae)

“…CTL, also known as immulectin, can not only participate in innate immunity, but also recognize pathogenic microorganisms and activate specific immunity ( Theopold et al, 1999 ; Koizumi et al, 1999 ), enhanced hemocyte encapsulation, melanization and enhanced phagocytic efficiency ( Kawasaki, Kawasaki & Yamashina, 1989 ; Yu et al, 2006 ). Sod plays a key role in resisting virus and bacterial invasion ( Shi et al, 2021 ), which can regulate the effect of cellular oxidative stress and participate in fat body immunity ( Nojima et al, 2015 ). Peptidoglycan recognition proteins (PGRPs) are a family that recognize peptidoglycan and participate in immune response, including pgrp-sa, pgrp-sb, pgrp-sc, pgrp-sd, pgrp-lc and pgrp-le ( Kurata, 2014 ).…”

Evolution and assembly of Anopheles aquasalis 's immune genes: primary malaria vector of coastal Central and South America and the Caribbean Islands

“…Another highly conserved family of proteins identified here relates to the metabolism of reactive oxygen species (ROS) products, as in the case of superoxide dismutase proteins (SOD). This group is universally present and is believed to have arisen long ago in prokaryotes from increased oxygen production in the atmosphere [ 50 , 51 ]. In An.…”