Molecular cloning, expression and characterization of the first three genes in the mevalonate-independent isoprenoid pathway in Streptomyces coelicolor

1-Deoxy-d-xylulose 5-Phosphate Synthase Catalyzes a Novel Random Sequential Mechanism

“…In addition, we required buffering conditions in which substrates are stable, DXP synthase activity is optimal, and collisional fluorescence quenching and nonspecific binding are minimized. A review of literature reports on DXP synthase reveals varying kinetic constants for the enzyme under a variety of conditions (6,12,13,19,20). Thus, a buffer optimization study was performed.…”

Improvement of Coenzyme Q10Production by Increasing the NADH/NAD+Ratio inAgrobacterium tumefaciens

“…7,10,11) The discovery of the eubacterial MEP pathway has provided a means of engineering of the isoprenoid flux to improve CoQs productivity. Some groups have reported that production of CoQ 8 and of lycopene in E. coli was enhanced by overexpression of 1-deoxy-D-xylulose 5-phosphate synthase (Dxs) 12) or 1-deoxy-D-xylulose 5-phosphate (DXP) reductoisomerase, the enzymes catalyzing the first and second steps of the MEP pathway respectively.…”

Growth-phase Dependent Expression of the Mevalonate Pathway in a Terpenoid Antibiotic-producingStreptomycesStrain

“…Very recently, Cane and coworkers have reported that Streptomyces coelicolor also had two distinct dxs genes. 17) They have cloned the two dxs genes by PCR and expressed their products as recombinant enzymes in E. coli. Although they were able to detect the expected activities with both of the recombinant enzymes, it remained unclear whether each of the two dxs genes was functionally expressed in Streptomyces strains.…”

“…The dxs genes encoding the DXP synthase have been cloned from Escherichia coli, 12,13) Rhodobacter capsulatus, 14) Pseudomonas aeruginosa, 15) Streptomyces sp. strain CL190, 16) Streptomyces coelicolor, 17) Synechococcus leopoliensis,…”