Molecular cloning, expression and polymorphism of gooseLRP8gene

Abstract

1. The low-density lipoprotein receptor-related protein 8 (LRP8), a member of the low-density lipoprotein receptor (LDLR) gene family, participates in the supplying of lipid during follicular development. The objective of the study was to identify and characterise the LRP8 gene in goose. 2. A 2867 bp fragment that covered the complete coding region (CDS) of goose (Anser cygnoides) LRP8 gene was cloned. It encoded a protein of 917 amino acid residues containing a 24-amino acid signal peptide and 5 functional domains. The goose LRP8 showed high nucleic acid and amino acid identities with those in other species. 3. Similarly to duck LRP8 gene, two splice variants of LRP8, LRP8-1 (containing 8 ligand-binding repeats) and LRP8-2 (containing 7 ligand-binding repeats), were identified in goose. 4. Semi-quantitative RT-PCR analysis indicates that the LRP8-1 transcript is expressed in heart, liver, spleen, lung, kidney, breast muscle, duodenum, hypothalamus, pituitary and ovary, negligible or absent in sebum and oviduct, and the LRP8-2 transcript is widely expressed in all examined tissues. 5. A total of 7 SNPs were identified in the coding region of the goose LRP8 gene.

Chicken ovarian follicles morphology and growth differentiation factor 9 gene expression in chicken ovarian follicles: review

“…The chosen follicle will develop faster from an F6 follicle to an F1 follicle until ovulation occurs. According to Wang et al. (2014) , the preovulatory ovarian follicles develop in order from F6 to F1, with F1 as the largest follicle, which is ready to be ovulated next and F2 as the second largest follicle followed by others until F6 as the smallest preovulatory follicle.…”

“…faster from an F6 follicle to an F1 follicle until ovulation occurs. According to Wang et al (2014), the preovulatory ovarian follicles develop in order from F6 to F1, with F1 as the largest follicle, which is ready to be ovulated next and F2 as the second largest follicle followed by others until F6 as the smallest preovulatory follicle. According to Yang et al (2019), Yangzhou geese follicle numbers are more than that in Zhejiang geese and Carlos geese in the egg-laying periods.…”