Molecular cloning, mRNA expression, and characterization of heat shock protein 10 gene from humphead snapper Lutjanus sanguineus

Zhang, Xinzhong; Dai, Liping; Wu, Zaohe; Jian, Jichang; Lu, Yuanan

doi:10.1016/j.margen.2010.10.001

Cited by 2 publications

(5 citation statements)

References 41 publications

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“…were observed in brain and hepatopancreas challenged with microorganisms (Gao et al, 2014;Zhang et al, 2011). In the present study, the transcriptional levels of the three sHSPs in hepatopancreas of P. trituberculatus injected with V. alginolyticus were upregulated rapidly at 3 h post injection.…”

Section: Discussionsupporting

confidence: 54%

“…As multifunctional molecules, HSPs can respond to various abiotic and biotic stress (Neckers & Tatu, 2008; Rhee et al, 2009; Roberts et al, 2010; Velázque‐Amado et al, 2012). In previous study, after challenged with microorganisms, the upregulations of transcriptional levels of several sHSPs have been observed in various invertebrates' species such as Tegillarca granosa , Lutjanus sanguineus , L. vannamei and Macrobrachium rosenbergii (Bao et al, 2011; Oliphant et al, 2012; Wang et al, 2017; Zhang et al, 2011). In contrast, the expression level of HSP20 downregulated in Fenneropenaeus chinensis and P. monodon challenged with white spot syndrome virus (WSSV) (Gao et al, 2014; Huang et al, 2008).…”

Section: Discussionmentioning

confidence: 90%

“…In contrast, the expression level of HSP20 downregulated in Fenneropenaeus chinensis and P. monodon challenged with white spot syndrome virus (WSSV) (Gao et al, 2014; Huang et al, 2008). In addition, no significant changes in the mRNA expression of ByHSP10 and FcHSP21 were observed in brain and hepatopancreas challenged with microorganisms (Gao et al, 2014; Zhang et al, 2011). In the present study, the transcriptional levels of the three sHSPs in hepatopancreas of P. trituberculatus injected with V .…”

Section: Discussionmentioning

confidence: 99%

“…vannamei and Macrobrachium rosenbergii (Bao et al, 2011;Oliphant et al, 2012;Wang et al, 2017;Zhang et al, 2011). In contrast, the expression level of HSP20 downregulated in Fenneropenaeus chinensis and P. monodon challenged with white spot syndrome virus (WSSV) (Gao et al, 2014;Huang et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

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Bacteria binding and antibacterial activities of three small heat shock protein from the swimming crab, Portunus trituberculatus

Zhang

Zheng

et al. 2022

Aquaculture Research

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The swimming crab, Portunus trituberculatus, is one of the most important commercial crabs cultured in China, with a total yield of approximately 100,000 tons (Fisheries Bureau of Agriculture Ministry of China, 2019). In recent decade, despite the increasing development of the P. trituberculatus farming industry, diseases caused by bacterial infection have caused tremendous economic losses and posed serious threats to the sustainable and healthy development of P. trituberculatus aquaculture industry. Excavating immune-related molecules from host, especially antibacterial molecules, will provide theoretical guidance and candidate therapeutic agents to address the disease problem.Heat shock proteins (HSPs), which widely exist in prokaryotes and eukaryotes, are molecular chaperones that play critical roles in maintaining cellular homeostasis (Schlesinger, 1990;Sikora & Grzesiuk, 2007). According to their homology and molecular mass, HSPs can be grouped into several families, including HSP110, HSP100, HSP90, HSP70, HSP60 and small molecular mass HSPs (sHSPs) (Craig et al., 1994;Tang et al., 2011). HSPs play a primary role

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Bacteria binding and antibacterial activities of three small heat shock protein from the swimming crab, Portunus trituberculatus

Zhang

Zheng

et al. 2022

Aquaculture Research

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“…On the basis of molecular weight and sequence similarity, Hsps can be usually divided into several families, including Hsp90, Hsp70, Hsp60, Hsp40 and small heat shock proteins (sHsps, molecular weights ranging from 12 to 43 kDa; (Kim et al ., 1998; Feder & Hofmann, 1999; Sørensen et al ., 2003; Huang et al ., 2008). Hsp100 and Hsp10 are also sometimes included in the Hsp superfamily (Jia et al ., 2011; Miot et al ., 2011; Zhang et al ., 2011).…”

Section: Introductionmentioning

confidence: 99%

Hsp70 and small Hsps are the major heat shock protein members involved in midgut metamorphosis in the common cutworm,Spodoptera litura

Shen

et al. 2012

Insect Molecular Biology

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Heat shock proteins (Hsps) are important chaperones, which are involved in various signal pathways and regulate lots of physiological processes. Early research suggested that some Hsps are involved in insect development. However, few studies have been carried out to explore the roles of Hsps, especially in larval-pupal metamorphosis. In the present study, 49 Hsp unigenes were identified in the Spodoptera litura transcriptome and their mRNA expression profiles during midgut metamorphosis were examined using a tag-based digital gene expression system. The genes with the most different levels of expression were then cloned and their expression patterns in midguts from sixth instar larvae to pupae were analysed using real time quantitative PCR. The responses of these genes to juvenile hormone (JH) and 20-hydroxyecdysone (20E) were also studied. The results showed that the mRNA levels of 22 Hsp unigenes changed significantly during midgut metamorphosis. Amongst these 22 unigenes, hsp70, hsp20.4 and hsp20.8 were the most up-regulated members, and hsp15.9, hsp19.3 and hsp22.0 were the most down-regulated ones. Further studies showed that hsp70, hsp20.4 and hsp20.8 were remarkably up-regulated by JH. In addition, 20E slightly increased the mRNA levels of both hsp20.4 and hsp20.8. However, hsp15.9, hsp19.3 and hsp22.0 did not respond to either JH or 20E. These results indicate that Hsp70 and small Hsps (sHsps) are probably the major players in midgut metamorphosis in S. litura. The current findings provide valuable insights into the roles of the Hsp superfamily in insect metamorphosis.

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Molecular cloning, mRNA expression, and characterization of heat shock protein 10 gene from humphead snapper Lutjanus sanguineus

Cited by 2 publications

References 41 publications

Bacteria binding and antibacterial activities of three small heat shock protein from the swimming crab, Portunus trituberculatus

Bacteria binding and antibacterial activities of three small heat shock protein from the swimming crab, Portunus trituberculatus

Hsp70 and small Hsps are the major heat shock protein members involved in midgut metamorphosis in the common cutworm,Spodoptera litura

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