Molecular cloning of low-temperature-inducible ribosomal proteins from soybean

Kim, Kee‐Young; Park, Seong‐Whan; Chung, Youngjin; Chung, Chung‐Han; Kim, Jung‐In; Lee, Jai‐Heon

doi:10.1093/jxb/erh125

Cited by 108 publications

(72 citation statements)

References 20 publications

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“…Unlike the cold responsive CRT/DRE binding factor (CBF) genes that are rapidly and markedly induced within 30 min of cold treatment , the RPL23a paralogs were induced slowly (¸24 h) and accumulated to a maximum of 150-250% of their pre-treatment levels. This result is in agreement with Wndings from soybean (Glycine max) where three ribosomal proteins, RPS6, RPS13 and RPL37, are upregulated within 3-9 days of cold treatment (Kim et al 2004). Additional support is provided by experiments showing upregulation of one or more r-protein transcripts in response to cold treatment of many plant species, including Arabidopsis (Jung et al 2003;Oono et al 2006), canola (Brassica napus, Saez-Vasquez et al 1993, sunXower (Helianthus annuus, Fernandez et al 2008) and trifoliate orange (Poncirus trifoliata, Zhang et al 2005).…”

Section: Discussionsupporting

confidence: 90%

Transcript profiling demonstrates absence of dosage compensation in Arabidopsis following loss of a single RPL23a paralog

Degenhardt

Bonham‐Smith

2008

Planta

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Translation of nucleus-encoded messages in plants is conducted by the cytoplasmic ribosome, an enzyme that is comprised of two RNA/protein subunits. In Arabidopsis thaliana, the 81 different ribosomal proteins (r-proteins) of the cytosolic ribosome belong to gene families with multiple expressed members. Given that ribosomes generally contain only one copy of each r-protein, regulatory mechanisms must exist to ensure their stoichiometric accumulation. These mechanisms must be dynamic, allowing for adjustments to ribosome biogenesis to fulfill biological requirements for protein synthesis during development, and following stress induction of global changes in gene expression. In this study, we investigated whether r-protein paralogs are feedback regulated at the transcript level by obtaining a T-DNA knockout of one member, RPL23aB, from the two-member RPL23a family. Expression of the lone functional paralog in this line, RPL23aA, was compared to the expression of both paralogs in wildtype plants under non-stressed, low temperature-, and high light stresses. RPL23aA expression was not upregulated in RPL23aB knockouts to compensate for paralog-loss, and consequently knockouts showed reduced total abundance of RPL23a transcripts. However, no phenotype developed in RPL23aB knockouts, suggesting that this paralog is dispensable under experimental conditions examined, or that compensation by RPL23aA may occur post-transcriptionally. Patterns of RPL23aA and RPL23aB transcript accumulation in wildtype plants suggest that paralogs respond coordinately to developmental and stress stimuli.

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Section: Discussionsupporting

confidence: 90%

Transcript profiling demonstrates absence of dosage compensation in Arabidopsis following loss of a single RPL23a paralog

Degenhardt

Bonham‐Smith

2008

Planta

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“…For example, plant ribosomal proteins S14, S15a, S16, L10, L13a, and L30 can be induced by diverse phytohormones, including auxin (32), cytokinin (32,33), and abscisic acid (33), and by stress-inducing stimuli, including heat (32), cold (32,34), and UVB stress (35). Recent analysis of the expression of RP genes and the differential accumulation of RPs in Arabidopsis roots upon phosphate or iron deficiency revealed that these 579 RP genes were associated mainly with abiotic stress responses.…”

Section: Discussionmentioning

confidence: 99%

Abiotic Stress Resistance, a Novel Moonlighting Function of Ribosomal Protein RPL44 in the Halophilic Fungus Aspergillus glaucus

Liu

Xie

et al. 2014

Appl Environ Microbiol

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bRibosomal proteins are highly conserved components of basal cellular organelles, primarily involved in the translation of mRNA leading to protein synthesis. However, certain ribosomal proteins moonlight in the development and differentiation of organisms. In this study, the ribosomal protein L44 (RPL44), associated with salt resistance, was screened from the halophilic fungus Aspergillus glaucus (AgRPL44), and its activity was investigated in Saccharomyces cerevisiae and Nicotiana tabacum. Sequence alignment revealed that AgRPL44 is one of the proteins of the large ribosomal subunit 60S. Expression of AgRPL44 was upregulated via treatment with salt, sorbitol, or heavy metals to demonstrate its response to osmotic stress. A homologous sequence from the model fungus Magnaporthe oryzae, MoRPL44, was cloned and compared with AgRPL44 in a yeast expression system. The results indicated that yeast cells with overexpressed AgRPL44 were more resistant to salt, drought, and heavy metals than were yeast cells expressing MoRPL44 at a similar level of stress. When AgRPL44 was introduced into M. oryzae, the transformants displayed obviously enhanced tolerance to salt and drought, indicating the potential value of AgRPL44 for genetic applications. To verify the value of its application in plants, tobacco was transformed with AgRPL44, and the results were similar. Taken together, we conclude that AgRPL44 supports abiotic stress resistance and may have value for genetic application.

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“…The capacity for thermal acclimation of RNA translation or protein synthesis appears limited, largely relying on production of additional ribosomes (Nomura et al, 1984;Fraser et al, 2002;Storch and Pörtner, 2003;Kim et al, 2004). Such a large difference in ribosomal protein enrichment implies large increases in cellular RNA at lower temperatures, as RNA:protein in ribosomes is constant.…”

Section: Enrichment Patterns At Low Temperaturementioning

confidence: 99%

Metatranscriptomes reveal functional variation in diatom communities from the Antarctic Peninsula

et al. 2015

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Functional genomics of diatom-dominated communities from the Antarctic Peninsula was studied using comparative metatranscriptomics. Samples obtained from diatom-rich communities in the Bransfield Strait, the western Weddell Sea and sea ice in the Bellingshausen Sea/Wilkins Ice Shelf yielded more than 500K pyrosequencing reads that were combined to produce a global metatranscriptome assembly. Multi-gene phylogenies recovered three distinct communities, and diatom-assigned contigs further indicated little read-sharing between communities, validating an assembly-based annotation and analysis approach. Although functional analysis recovered a core of abundant shared annotations that were expressed across the three diatom communities, over 40% of annotations (but accounting for o10% of sequences) were community-specific. The two pelagic communities differed in their expression of N-metabolism and acquisition genes, which was almost absent in post-bloom conditions in the Weddell Sea community, while enrichment of transporters for ammonia and urea in Bransfield Strait diatoms suggests a physiological stance towards acquisition of reduced N-sources. The depletion of carbohydrate and energy metabolism pathways in sea ice relative to pelagic communities, together with increased light energy dissipation (via LHCSR proteins), photorespiration, and NO 3 − uptake and utilization all pointed to irradiance stress and/or inorganic carbon limitation within sea ice. Ice-binding proteins and cold-shock transcription factors were also enriched in sea ice diatoms. Surprisingly, the abundance of gene transcripts for the translational machinery tracked decreasing environmental temperature across only a 4°C range, possibly reflecting constraints on translational efficiency and protein production in cold environments.

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Molecular cloning of low-temperature-inducible ribosomal proteins from soybean

Cited by 108 publications

References 20 publications

Transcript profiling demonstrates absence of dosage compensation in Arabidopsis following loss of a single RPL23a paralog

Transcript profiling demonstrates absence of dosage compensation in Arabidopsis following loss of a single RPL23a paralog

Abiotic Stress Resistance, a Novel Moonlighting Function of Ribosomal Protein RPL44 in the Halophilic Fungus Aspergillus glaucus

Metatranscriptomes reveal functional variation in diatom communities from the Antarctic Peninsula

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