Molecular cloning, recombinant protein expression, tissue distribution and functional analysis of a new c-type lysozyme from Lezhi black goat rumen

ZhangPeng,; WangYong,; JiangMingfeng,; ZhuLianlian,; LiJianbo,; LuoMeirong,; RenHonghui,; LiuLushu,

doi:10.4141/cjas2012-150

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…The authors explained this result by concluding that there is a more recent common ancestor of at least one of the stomach lysozymes with milk lysozyme than with other stomach lysozymes. The interest in ruminant animals is also confirmed in a study [ 30 ] of a new c-type lysozyme from Lezhi black goat rumen (147 amino acid residues) sharing 70.27% of its identity with the capra hircus blood lysozyme that, likely, functions in host immunity and digestive systems.…”

Section: Sourcesmentioning

confidence: 89%

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Ferraboschi

Ciceri

Grisenti³

2021

Antibiotics

207

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Lysozyme is a ~14 kDa protein present in many mucosal secretions (tears, saliva, and mucus) and tissues of animals and plants, and plays an important role in the innate immunity, providing protection against bacteria, viruses, and fungi. Three main different types of lysozymes are known: the c-type (chicken or conventional type), the g-type (goose type), and the i-type (invertebrate type). It has long been the subject of several applications due to its antimicrobial properties. The problem of antibiotic resistance has stimulated the search for new molecules or new applications of known compounds. The use of lysozyme as an alternative antibiotic is the subject of this review, which covers the results published over the past two decades. This review is focused on the applications of lysozyme in medicine, (the treatment of infectious diseases, wound healing, and anti-biofilm), veterinary, feed, food preservation, and crop protection. It is available from a wide range of sources, in addition to the well-known chicken egg white, and its synergism with other compounds, endowed with antimicrobial activity, are also summarized. An overview of the modified lysozyme applications is provided in the form of tables.

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Section: Sourcesmentioning

confidence: 89%

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Ferraboschi

Ciceri

Grisenti³

2021

Antibiotics

207

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“…It seems that the YML expressed only as native signal peptide without an expression tag from pET32 vector can improve the enzyme activity when the heterologous YML was expressed in E. coli. Recombinant lysozyme expressed in E. coli was reported in several papers ( Zhang et al, 2014 ). There are 8 cysteines in YML which form four disulfide bonds.…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Characterization of a New C-type Lysozyme Gene from Yak Mammary Tissue

Jiang

Ren

et al. 2015

Asian Australas. J. Anim. Sci

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Milk lysozyme is the ubiquitous enzyme in milk of mammals. In this study, the cDNA sequence of a new chicken-type (c-type) milk lysozyme gene (YML), was cloned from yak mammary gland tissue. A 444 bp open reading frames, which encodes 148 amino acids (16.54 kDa) with a signal peptide of 18 amino acids, was sequenced. Further analysis indicated that the nucleic acid and amino acid sequences identities between yak and cow milk lysozyme were 89.04% and 80.41%, respectively. Recombinant yak milk lysozyme (rYML) was produced by Escherichia coli BL21 and Pichia pastoris X33. The highest lysozyme activity was detected for heterologous protein rYML5 (M = 1,864.24 U/mg, SD = 25.75) which was expressed in P. pastoris with expression vector pPICZαA and it clearly inhibited growth of Staphylococcus aureus. Result of the YML gene expression using quantitative polymerase chain reaction showed that the YML gene was up-regulated to maximum at 30 day postpartum, that is, comparatively high YML can be found in initial milk production. The phylogenetic tree indicated that the amino acid sequence was similar to cow kidney lysozyme, which implied that the YML may have diverged from a different ancestor gene such as cow mammary glands. In our study, we suggest that YML be a new c-type lysozyme expressed in yak mammary glands that plays a role as host immunity.

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“…The c-type lysozymes are the most representative lysozymes in nature, with the largest number of related studies, and chicken egg white c-type lysozyme is the main industrial source of lysozymes . In addition to human c-type lysozyme, in recent years, there have been lysozyme studies on cattle, sheep, deer, baboons, and bats in mammals. − In other vertebrates, besides chicken egg c-type lysozyme, there are also studies on c-type lysozymes in birds, such as wood ducks, turkeys, pigeons, quails, and lovebirds. − Besides mammals and birds, there are a large number of studies about other vertebrates, including fish c-type lysozymes . In addition to vertebrates, two c-type lysozyme genes were found in amphioxus .…”

Section: Classification Of Fish Lysozymesmentioning

confidence: 99%

Lysozymes in Fish

Song

Xiao

et al. 2021

J. Agric. Food Chem.

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In recent years, the deterioration of the aquaculture ecological environment has led to a high incidence of fish diseases. Lysozymes, important antimicrobial enzymes, play an important role in the innate immune system of fish. The studies of fish lysozymes benefit the control of fish infections caused by pathogens. In this review, we reviewed recent progress in fish lysozymes, including their classification, structural characteristics, biological functions and mechanisms, tissue distributions, and properties of their recombinant proteins, which will help us to systematically understand the fish lysozymes and facilitate their applications in the fields of food and agriculture.

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Molecular cloning, recombinant protein expression, tissue distribution and functional analysis of a new c-type lysozyme from Lezhi black goat rumen

Cited by 3 publications

References 19 publications

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Molecular Cloning and Characterization of a New C-type Lysozyme Gene from Yak Mammary Tissue

Lysozymes in Fish

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