2014
DOI: 10.2323/jgam.60.234
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Molecular cloning, sequence analysis and expression of a GHF 43 xylanase from Aspergillus niger in Escherichia coli

Abstract: A new xylanase gene (xyn43A) from Aspergillus niger XZ-3S was cloned and expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL. The coding region of the gene was separated by only one intron 86 bp in length. It encoded 318 amino acid residues of a protein with a calculated molecular weight (MW) of 33.47 kDa plus a signal peptide of 19 amino acids. The amino acid sequence of the xyn43A gene showed 77.56% amino acid identity to A. nidulans xylanase, and the phylogenetic tree analysis revealed that xyn43A had cl… Show more

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“…Xylanase is the key enzyme for xylan degradation and it belongs to glycoside hydrolase (GH) families 5, 7, 8, 10, 11, and 43 (Kim et al, 2018a;Nordberg Karlsson et al, 2018;Zhou et al, 2014). Xylanase belongs to GH10 and GH11 families are mainly endo-β-1,4-xylanases (Brusa et al, 2018;Bu et al, 2018;Yang et al, 2018).…”
Section: Introductionmentioning
confidence: 99%
“…Xylanase is the key enzyme for xylan degradation and it belongs to glycoside hydrolase (GH) families 5, 7, 8, 10, 11, and 43 (Kim et al, 2018a;Nordberg Karlsson et al, 2018;Zhou et al, 2014). Xylanase belongs to GH10 and GH11 families are mainly endo-β-1,4-xylanases (Brusa et al, 2018;Bu et al, 2018;Yang et al, 2018).…”
Section: Introductionmentioning
confidence: 99%