Molecular Cloning, Structure, and Expression of Mouse Estrogen-responsive Finger Protein Efp

Orimo, Akira; Inoue, Satoshi; Ikeda, Kazuhiro; Noji, Sumihare; Muramatsu, Masami

doi:10.1074/jbc.270.41.24406

Cited by 69 publications

(54 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The positive immunohistochemical staining for ER was observed in the nuclei of glandular and luminal epithelium and myometrium in rat uterus. This result was consistent with previous reports (Zaino et al 1989, Hild-Petito et al 1988, Hild-Petito et al 1992, Koji & Brenner 1993, Orimo et al 1995, Saunders et al 1997. The staining of ER was detected in the nuclei of glandular epithelium in rat uterus, whereas no staining was detected in luminal epithelium and myometrium.…”

Section: Discussionsupporting

confidence: 83%

“…Data from several laboratories, using in situ hybridization or immunohistochemistry, have shown that the ER localizes in granulosa cells and theca cells in certain species (Kudolo et al 1984, Iwai et al 1991, Billiar et al 1992, Orimo et al 1995, Byers et al 1997. Saunders et al (1997) were unable to demonstrate the presence of ER in rat granulosa cells by immunohistochemistry.…”

Section: Discussionmentioning

confidence: 99%

“…The cell transfection and nuclear extracts preparation from the cells were performed as described by Inoue et al (1993a). Nuclear extracts from rat tissues were prepared as described by Orimo et al (1995).…”

Section: Antibody Preparationmentioning

confidence: 99%

See 2 more Smart Citations

Differential immunolocalization of estrogen receptor alpha and beta in rat ovary and uterus

Hiroi¹,

Inoue²,

Watanabe³

et al. 1999

Journal of Molecular Endocrinology

135

View full text Add to dashboard Cite

In order to investigate the localization of estrogen receptor (ER) and ER in the reproductive organs in the rat, polyclonal antibodies were raised to each specific amino acid sequence. The Western blot with anti-ER antibody showed a 66 kDa band in rat ovary and uterus, while that with anti-ER antibody detected a 55 kDa band in rat ovary, uterus and prostate. The ligand-independent nuclear localization of the two receptors was verified by immunocytochemistry. By immunohistochemistry, the nuclei of glandular and luminal epithelial cells in the uterus were stained with anti-ER antibody, whereas only the nuclei of glandular epithelium cells were stained with anti-ER antibody. In rat ovary, positive signals were shown with anti-ER antibody in the nuclei of granulosa cells. No specific immunostaining was observed with anti-ER antibody. Although ER was immunostained at the proestrous, metestrous and diestrous stages, the immunoreactivity of ER was hardly detected at the estrous stage in rat ovary. Thus, we show differential expression of ER and ER in rat uterus and ovary at the protein level, which may provide a clue for understanding the roles of the two receptors in reproductive organs.

show abstract

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Differential immunolocalization of estrogen receptor alpha and beta in rat ovary and uterus

Hiroi¹,

Inoue²,

Watanabe³

et al. 1999

Journal of Molecular Endocrinology

135

View full text Add to dashboard Cite

show abstract

“…Furthermore, the expression of the estrogenresponsive finger protein (efp) is regulated during osteoblast differentiation (71). The ability of these proteins to induce differentiation could be related to their DNA binding properties, suggesting a role in transcriptional regulation, as described previously for efp (72). However, the sequences of the promoters targeted by these factors remain to be determined.…”

Section: Fig 5 Expression Of Enterophilin-1 Along the Small Intestimentioning

confidence: 95%

Enterophilins, a New Family of Leucine Zipper Proteins Bearing a B30.2 Domain and Associated with Enterocyte Differentiation

Gassama‐Diagne

Hullin-Matsuda

et al. 2001

Journal of Biological Chemistry

View full text Add to dashboard Cite

Enterocyte terminal differentiation occurs at the crypt-villus junction through the transcriptional activation of cell-specific genes, many of which code for proteins of the brush border membrane such as intestinal alkaline phosphatase, sucrase-isomaltase, or the microvillar structural protein villin. Several studies have shown that this sharp increase in specific mRNA levels is intimately associated with arrest of cell proliferation. We isolated several clones from a guinea pig intestine cDNA library. They encode new proteins characterized by an original structure associating a carboxyl-terminal B30.2/RFP-like domain and a long leucine zipper at the amino terminus. The first member of this novel gene family codes for a 65-kDa protein termed enterophilin-1, which is specifically expressed in enterocytes before their final differentiation. Enterophilin-1 is the most abundant in the small intestine but is still present in significant amounts in colonic enterocytes. In Caco-2 cells, a similar 65-kDa protein was recognized by a specific anti-enterophilin-1 antibody, and its expression was positively correlated with cell differentiation status. In addition, transfection of HT-29 cells with enterophilin-1 full-length cDNA slightly inhibited cell growth and promoted an increase in alkaline phosphatase activity. Taken together, these data identify enterophilins as a new family of proteins associated with enterocyte differentiation.The self-renewing small intestinal epithelium represents an attractive and valuable system to study various processes occurring during cell life such as proliferation, differentiation, or apoptosis. Proliferation is limited to the crypts of Lieberkü hn, where multipotent stem cells achieve continuous renewal of four main epithelial lineages. At the top of the crypt, cells lose their proliferative ability and complete differentiation during a highly organized migration along the crypt-villus axis. Enterocytes, mucus-producing goblet cells, and enteroendocrine cells evolve during a vertical migration to the villus apex (1-3). This process requires 4 to 5 days and results in well-differentiated cells that are finally released into the intestinal lumen upon programmed cell death. In contrast, Paneth cells undergo terminal differentiation while moving down to the base of the crypt (4 -8). The differentiation process and the function of the epithelium also vary along the horizontal axis (from proximal to distal intestine), and in both cases, functional differences reflect various patterns of gene expression as well as the nature of the epithelial cell type (7).How these various events are regulated at the genomic level and the intracellular signaling pathways involved in this differentiation process are still poorly understood. Recently identified genes were found to be necessary either to maintain the proliferative status of stem cells (Tcf-4) (9) or to direct the differentiation process (homeobox transcription factors cdx1 and cdx2) (10 -12). Most of the previous studies were performed with entero...

show abstract

“…4 In mice, estrogen-induced expression of Efp is observed in tissues such as uterine tissue. 5 Efp-deficient mice exhibit underdeveloped uteri and reduced estrogen responsiveness. 6 In human MCF-7 breast cancer cells, the expression of Efp mRNA is induced in response to estrogen.…”

Section: Introductionmentioning

confidence: 99%

Knockdown of Efp by DNA-modified small interfering RNA inhibits breast cancer cell proliferation and in vivo tumor growth

et al. 2010

View full text Add to dashboard Cite

The estrogen-responsive gene Efp promotes the growth of breast cancer cells by stimulating the degradation of a negative cell-cycle regulator, 14-3-3s, and is hence considered a suitable molecular target for breast cancer therapy. The use of small interfering RNA (siRNA) and its derivatives to silence cancer-related genes is being investigated with the aim of identifying clinical applications for these molecules. Recently, it has been shown that DNA-modified siRNA (chimeric siRNA) has good potential in clinical applications, because it induces fewer off-target effects or immune responses in mammalian cells. In the present study, we identified the most specific and effective siRNA (siEfp-1) for silencing Efp expression in MCF-7 breast cancer cells. For this purpose, we used an algorithm that primarily eliminates off-target effects. siEfp-1 considerably suppressed the in vitro proliferation and cell-cycle progression of MCF-7 cells, as well as the in vivo growth of MCF-7 tumors, in athymic mice. DNA-modified siEfp-1 (chimeric siEfp) significantly inhibited the expression of Efp, proliferation of cultured cells and the in vivo growth of MCF-7-derived tumors in athymic mice. In addition, the silencing of Efp expression by siEfp-1 and chimeric siEfp increased the expression of the 14-3-3s protein. These results suggest that siEfp-1 and chimeric siEfp could be useful in breast cancer therapy. Chimeric siEfp, in particular, has a high specificity and induces few side effects and is therefore expected to be used as a novel nucleic acid-based therapeutic agent.

show abstract

Molecular Cloning, Structure, and Expression of Mouse Estrogen-responsive Finger Protein Efp

Cited by 69 publications

References 55 publications

Differential immunolocalization of estrogen receptor alpha and beta in rat ovary and uterus

Differential immunolocalization of estrogen receptor alpha and beta in rat ovary and uterus

Enterophilins, a New Family of Leucine Zipper Proteins Bearing a B30.2 Domain and Associated with Enterocyte Differentiation

Knockdown of Efp by DNA-modified small interfering RNA inhibits breast cancer cell proliferation and in vivo tumor growth

Contact Info

Product

Resources

About