Molecular Colocalization Using Massive Gold Cluster Secondary Ion Mass Spectrometry

Eller, Michael J.; Vinjamuri, Anita; Tomlin, Bryan E.; Schweikert, E. A.

doi:10.1021/acs.analchem.8b02950

Cited by 13 publications

(24 citation statements)

References 31 publications

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“…) have also been shown to enhance pseudo-molecular ion detection due to lower energy sputtering of secondary species [8].…”

Section: +mentioning

confidence: 99%

“…Metal cluster ion beams such as Au 3 + and Bi 3 + have supplanted the traditional monoatomic ion beams (e.g., Ga + ), as they provide non-linear enhancements of secondary ion signal, as the cluster size increases while still being relatively easy to focus [ 6 , 7 ]. Very large, high energy gold clusters (e.g., Au 2800 8+ ) have also been shown to enhance pseudo- molecular ion detection due to lower energy sputtering of secondary species [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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(CO2)n+, (H2O)n+, and (H2O)n+ (CO2) gas cluster ion beam secondary ion mass spectrometry: analysis of lipid extracts, cells, and Alzheimer’s model mouse brain tissue

et al. 2021

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This work assesses the potential of new water cluster-based ion beams for improving the capabilities of secondary ion mass spectrometry (SIMS) for in situ lipidomics. The effect of water clusters was compared to carbon dioxide clusters, along with the effect of using pure water clusters compared to mixed water and carbon dioxide clusters. A signal increase was found when using pure water clusters. However, when analyzing cells, a more substantial signal increase was found in positive ion mode when the water clusters also contained carbon dioxide, suggesting that additional reactions are in play. The effects of using a water primary ion beam on a more complex sample were investigated by analyzing brain tissue from an Alzheimer’s disease transgenic mouse model. The results indicate that the ToF-SIMS results are approaching those from MALDI as ToF-SIMS was able to image lyso-phosphocholine (LPC) lipids, a lipid class that for a long time has eluded detection during SIMS analyses. Gangliosides, sulfatides, and cholesterol were also imaged. Graphical abstract

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“…) have also been shown to enhance pseudo-molecular ion detection due to lower energy sputtering of secondary species [8].…”

Section: +mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

(CO2)n+, (H2O)n+, and (H2O)n+ (CO2) gas cluster ion beam secondary ion mass spectrometry: analysis of lipid extracts, cells, and Alzheimer’s model mouse brain tissue

et al. 2021

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“…The description of the experimental device has been published previously. 36 Briefly, a liquid metal ion source produces a range of Au cluster projectiles. A Wien filter is used to select the Au 2800 8+ projectiles which are accelerated toward the target obtaining a final kinetic energy of 1040 keV.…”

Section: ■ Materials and Methodsmentioning

confidence: 92%

“…The description of the experimental device has been published previously . Briefly, a liquid metal ion source produces a range of Au cluster projectiles.…”

Section: Materials and Methodsmentioning

confidence: 99%

Nanoprojectile Secondary Ion Mass Spectrometry for Analysis of Extracellular Vesicles

et al. 2021

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We describe a technique based on secondary ion mass spectrometry with nanoprojectiles (NP-SIMS) for determining the protein content of extracellular vesicles, EVs, via tagged antibodies. The technique uses individual gold nanoprojectiles (e.g., Au400 4+ and Au2800 8+), separated in time and space, to bombard a surface. For each projectile impact (10–20 nm in diameter), the co-emitted molecules are mass analyzed and recorded as an individual mass spectrum. Examining these individual mass spectra for co-localized species allows for nanoscale mass spectrometry to be performed. The high lateral resolution of this technique is well suited for analyzing nano-objects. SIMS is generally limited to analyzing small molecules (below ∼1500 Da); therefore, we evaluated three molecules (eosin, erythrosine, and BHHTEGST) as prospective mass spectrometry tags. We tested these on a model surface comprising a mixture of all three tags conjugated to antibodies and found that NP-SIMS could detect all three tags from a single projectile impact. Applying the method, we tagged two surface proteins common in urinary EVs, CD63 and CD81, with anti-CD63–erythrosine and anti-CD81–BHHTEGST. We found that NP-SIMS could determine the relative abundance of the two proteins and required only a few hundred or thousand EVs in the analysis region to detect the presence of the tagged antibodies.

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“…47 MetA SIMS has also been shown useful to reduce the inuence of the chemical environment on the ion yields (referred to as a change of the "matrix effect" in the literature). 48 MetA SIMS has enhanced small molecule ion detection on biolms 49 as well as the signal of small organic compounds such as peptides 50 and additive molecules in tissue. 51,52 Typically, the thickness of the gold coating is about 1 nm.…”

Section: Signal Enhancement Methodsmentioning

confidence: 99%

A concise tutorial review of TOF-SIMS based molecular and cellular imaging

Massonnet

Heeren

2019

J. Anal. At. Spectrom.

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Molecular Colocalization Using Massive Gold Cluster Secondary Ion Mass Spectrometry

Cited by 13 publications

References 31 publications

(CO2)n+, (H2O)n+, and (H2O)n+ (CO2) gas cluster ion beam secondary ion mass spectrometry: analysis of lipid extracts, cells, and Alzheimer’s model mouse brain tissue

(CO2)n+, (H2O)n+, and (H2O)n+ (CO2) gas cluster ion beam secondary ion mass spectrometry: analysis of lipid extracts, cells, and Alzheimer’s model mouse brain tissue

Nanoprojectile Secondary Ion Mass Spectrometry for Analysis of Extracellular Vesicles

A concise tutorial review of TOF-SIMS based molecular and cellular imaging

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