Molecular cytogenetic characterization and SSR marker analysis of a leaf rust resistant wheat line carrying a 6G(6B) substitution from Triticum timopheevii (Zhuk.)

Abstract

A disease (powdery mildew, leaf rust) resistant line was selected from the progenies of a Triticum aestivum 9 Triticum timopheevii amphiploid produced at Martonvásár. This line was previously identified with C-banding as a 6G(6B) substitution. In order to detect the 6G chromosome in a wheat background, fluorescence in situ hybridization (FISH) and microsatellite marker analysis were used. Ten microsatellite markers of the 43 tested generated PCR products that were polymorphic between chromosomes 6B and 6G, and four showed lengthpolymorphism. The FISH hybridization pattern of 6G from T. timopheevii was identified using a combination of four repetitive DNA probes (Afa-family, pSc119.2, pTa71, (GAA) 7 ). Genomic in situ hybridization (GISH) technique, capable of labelling the A t and G genomes separately, was used on the same slides to differentiate the A t and G genomes in T. timopheevii. The A t and G genomes of T. timopheevii were grouped on the basis of the GISH patterns and a cyclic intergenomic translocation involving 6A t -1G-4G was detected in T. timopheevii accession TRI667. The presence of 6G in the substitution line was demonstrated using FISH with the four repetitive DNA probes. Chromosome 6G was clearly identified and its FISH pattern was different from that of 6B in the parental wheat cultivar Fleischmann-481. According to field tests, the 6G(6B) substitution line has resistance to leaf rust.

Molecular cytogenetic identification and phenotypic description of a new synthetic amphiploid, Triticum timococcum (AtAtGGAmAm)

“…2 b). Like its female parent, T. timococcum carries the same species-specific translocations previously reported by Rodríguez et al ( 2000 ) and Uhrin et al ( 2012 ). The short arm of chromosome 6A t has a long translocated segment from the short arm of chromosome 1G, while 1G has a short segment from 6A t (6A t S/1GS).…”

“…Chromosome discrimination was carried out using the previously published FISH patterns of T. timopheevii (Jiang and Gill 1994 ; Uhrin et al 2012 ) and T. monococcum (Megyeri et al 2012 ). Chromosomes 6G, 6A t , 1A m and 5A m showed very strong pTa71 signals, while the green coloured pSc119.2 probe gave strong signals on all the G genome chromosomes and weaker signals on two A t chromosomes (1A t and 5A t ).…”

“…The discrimination of A genome chromosomes was based on the findings of Megyeri et al ( 2012 ), while the identification of G chromosomes was aided by the results of Uhrin et al ( 2012 ).…”

Development and Molecular Cytogenetic Identification of a Novel Wheat–Leymus mollis Lm#7Ns (7D) Disomic Substitution Line with Stripe Rust Resistance

“…FISH is a powerful technique for localizing highly repetitive DNA sequences, detecting specific sites in particular regions of individual chromosomes and discriminate genome constitutions [ 32 , 33 ]. Many substitution lines related to common wheat chromosomes and alien chromosomes have been identified using FISH or nulli-tetrasomic analysis, including 6 G (6 B ) [ 34 ], 1St( 1 D ) [ 28 ], 6 Js /6 B [ 13 ], 2 R a (2 D ) [ 35 ], 2 Ns (2 D ) [ 36 ], 2 V b (2 D ) [ 37 ], and 3 D (3 Ns ) [ 18 ]. In this study, the standard FISH karyotype patterns of wheat parent 7182 was painted for the first time using oligonucleotide probes Oligo-pTa535 and Oligo-pSc119.2, which was convenient to distinguish common wheat chromosomes in M11003-3-1-15-8.…”

Identification of a novel wheat-Thinopyrum ponticumaddition line revealed with cytology, SSR, EST-SSR, EST-STS and PLUG markers

“…ponticum, where there is a lack of molecular biology information, the transfer of wheat molecular markers to the species is a feasible method for genetic analysis (Dobrovolskaya et al 2009). Over the last two decades, SSR markers have been used extensively as a effective tool for the genetic analysis of alien chromosomes in various species, such as Thinopyrum ponticum (Hu et al 2011), Thinopyrum intermedium (Lin et al 2007), Triticum timopheevii (Uhrin et al 2012), Leymus mollis (Zhao et al 2013) and Secale cereale (Tang et al 2008).…”