Molecular cytogenetic characterization of wheat-Thinopyrum ponticum translocations bearing blue-grained gene(s) induced by r-ray

Abstract

Eight γ -irradiation-induced Triticum aestivum -Thinopyrum ponticum translocations conveying the blue aleurone were characterized using molecular cytogenetic approach. The size of alien chromosome segments was estimated by genomic in situ hybridization (GISH). The wheat chromosome segments involved in these translocations were clearly identified by two-color fluorescence in situ hybridization (FISH) with the probes of pAs1 and pSc119.2 (or pHvG38). Most of the detected translocations were reciprocal translocations involving wheat chromosomes 1B, 2D, 3A, 4A, 5B, 6B, 6D and 7A. This series of bluegrained wheat translocation lines would be useful in theoretical studies and wheat chromosome engineering breeding.

Chromosome and genome composition of a Triticum × Thinopyrum hybrid by classical and molecular cytogenetic techniques

“…The characteristic pattern of the 2D and 4D chromosomes of wheat, with centromeric signals (Brasileiro-Vidal et al 2003;Molnár et al 2009;Mukai et al 1993;Rayburn and Gill 1986;Schneider et al 2003;Sepsi et al 2008;Wang et al 2005;Zheng et al 2006) allowed us to detect the presence of these two pairs of chromosomes in trigopiro SH16 INTA.…”

The colours of durum wheat: a review

“…The gene for blue aleurone was transferred from the tall wheatgrass Agropyron elongatum to bread wheat (Triticum aestivum L.) (Zeller et al 1991;Morrison et al 2004). Similarly, translocation lines of the tall wheatgrass Thinopyrum ponticum with the blue aleurone gene(s) have also been characterised (Zheng et al 2006).…”

Caracterización del genoma de un Triticum x Thinopyrum (Poaceae) sintético amfiploide utilizando hibridación in situ.

“…In situ hybridization with genomic DNA (GISH) has been previously used to determine the genome composition of many interspecific hybrids, as well as to detect the presence of introgressions and translocations (Ferrari et al, 2005;Wang et al, 2005;Zheng et al, 2006;Chen et al, 1998;Qi et al, 2010).…”