Molecular Detection and Characterization of Salmonella Gallinarum From Poultry Farms in Brazil

Zanetti, Nathalie; Carli, Sílvia De; Souza, Margarida Neves; Lehmann, Fernanda Kieling Moreira; Kipper, Diéssy; Dias, K.K.R.; Fonseca, André Salvador Kazantzi; Lunge, Vagner Ricardo; Ikuta, Nilo

doi:10.3382/japr/pfz051

Cited by 6 publications

(5 citation statements)

References 21 publications

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“…Para a determinação do pH, utilizou-se 50 g de cada amostra homogeneizada com 10 mL de água destilada, sendo a leitura realizada através do potenciômetro de um pHmetro de bancada previamente calibrado (Brasil, 1981 Os isolados suspeitos foram confirmados pela técnica de PCR, tendo como alvo o gene ompC (Dai et al, 2018). Através do método de fervura (Zanetti et al, 2019) extraiuse o DNA, que foi utilizado em reação posterior composta por GoTaq Green Master Mix, água estéril livre de nuclease e primer(i) (ompC-F 5"-ATCGCTGACTTATGCA ATCG-3", ompC-R 5"-CGGGTTGCGTTATAGGTCTG-3").…”

Section: Methodsunclassified

Avaliação Da Adequação Das Boas Práticas De Fabricação E Qualidade De Carne Bovina Em Açougues

Ventura

Costa

Dias

et al. 2020

AVS

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Este estudo teve como objetivo avaliar a adoção das boas práticas de fabricação e parâmetros físico-químicos (pH e temperatura) e microbiológico (presença de Salmonella spp.) da carne moída bovina comercializada em açougues de Uberlândia, MG. Foram analisados 40 estabelecimentos das cinco regiões do município (oito por região), sendo as boas práticas de fabricação avaliada por meio de um checklist (A. Edificações e Instalações; B. Equipamentos, móveis e utensílios; e C. Manipuladores). Para as análises físico-químicas e microbiológica foram coletadas 200g de carne moída bovina. Os estabelecimentos apresentaram 36,5% de inconformidade quanto à adoção das boas práticas de fabricação, sendo a área externa com focos de insalubridade e a lavagem das mãos os principais problemas observados. Em relação ás análises físico-químicas, identificou-se 75% de inconformidade dentre os açougues avaliados, sendo constatada também a presença de Salmonella spp. em amostras de carne de dois deles (5%). Conclui-se que existem falhas na adoção de boas práticas de fabricação e qualidade da carne analisada, e adequações são necessárias para a comercialização de alimentos seguros ao consumidor.

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Section: Methodsunclassified

Avaliação Da Adequação Das Boas Práticas De Fabricação E Qualidade De Carne Bovina Em Açougues

Ventura

Costa

Dias

et al. 2020

AVS

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“…Poultry health and welfare are very important for food supply and safety and significantly influence economic and societal prosperity. In many countries, official data regarding disease prevalence is poor as the diseases could be under-reported, thus, the probability incidence of PD and FT is underestimated (Zanetti et al, 2019;de Mesquita Souza Saraiva et al, 2022). Though has not been reported from Norway, Finland, Greece, and Estonia.…”

Section: Prevalence Of Fowl Typhoidmentioning

confidence: 99%

Fowl Typhoid: Present Scenario, Diagnosis, Prevention and Control Measures

2023

IJAB

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Poultry farming is facing complicated diseases including salmonellosis throughout the world. Avian salmonellosis renders heavy economic losses not only by causing high mortality (maybe up to 80%) but also by a reduction in meat and egg production. Salmonella has zoonotic importance. There are more than 2500 species of Salmonella, among these species important for poultry are Salmonella Gallinarum, S. pullorum, S. Typhimurium, S. enteritidis, etc. Among these Salmonella Gallinarum causes fowl typhoid (FT). FT affects mostly adult chickens resulting in greenish diarrhea and enteritis and high mortality. In the past, only antimicrobial drugs were used to control Salmonella in poultry which was causing also a threat to human beings as residues of the drugs were coming in meat and eggs. Day by day, antimicrobial resistance is developing against Salmonella in poultry, thus new ways and means are being searched for its control. Both live and inactivated vaccines have been in application against S. Gallinarum. Previously, Salmonella species were diagnosed by traditional ways of isolation, biochemical reactions, and microscopy. With the passage of time, there are new advancements in its diagnosis. Many techniques have been adopted for the detection of the causative agent. The PCR is useful for the rapid and correct diagnosis of Salmonella serotypes in poultry. Multiplex-PCR, Real-time PCR, PCR-RFLP assay, capillary gel electrophoresis (CGE), PFGE, indirect ELISA, and RAPD DNA are being used for diagnosis. Various strategies have been applied to curtail or control Salmonella infection in poultry. As Salmonella antibiotic resistance is developing, alternative treatment/prevention strategies are being opted for. Organic compounds such as prebiotics, probiotics, and phytobiotics are in use to limit salmonella growth. Organic compounds have several benefits to the chicken, and eggs and meats are free from residues and have public health safety. Omic technologies and bacteriophages as an alternative solution to control Salmonellosis have also been suggested.

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“…Gallinarum causes FT, an acute septicemic or chronic disease that occurs most often in adult birds through horizontal transmission. Acute septicemic and/or chronic FT is responsible for 40% of death in Brazilian poultry flocks [73,74]. FT also induces significant economic losses by reducing fertility and egg production [8].…”

Section: Salmonella Gallinarummentioning

confidence: 99%

Emergence, Dissemination and Antimicrobial Resistance of the Main Poultry-Associated Salmonella Serovars in Brazil

Kipper

Mascitti

Carli

et al. 2022

Veterinary Sciences

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Salmonella infects poultry, and it is also a human foodborne pathogen. This bacterial genus is classified into several serovars/lineages, some of them showing high antimicrobial resistance (AMR). The ease of Salmonella transmission in farms, slaughterhouses, and eggs industries has made controlling it a real challenge in the poultry-production chains. This review describes the emergence, dissemination, and AMR of the main Salmonella serovars and lineages detected in Brazilian poultry. It is reported that few serovars emerged and have been more widely disseminated in breeders, broilers, and layers in the last 70 years. Salmonella Gallinarum was the first to spread on the farms, remaining as a concerning poultry pathogen. Salmonella Typhimurium and Enteritidis were also largely detected in poultry and foods (eggs, chicken, turkey), being associated with several human foodborne outbreaks. Salmonella Heidelberg and Minnesota have been more widely spread in recent years, resulting in frequent chicken/turkey meat contamination. A few more serovars (Infantis, Newport, Hadar, Senftenberg, Schwarzengrund, and Mbandaka, among others) were also detected, but less frequently and usually in specific poultry-production regions. AMR has been identified in most isolates, highlighting multi-drug resistance in specific poultry lineages from the serovars Typhimurium, Heidelberg, and Minnesota. Epidemiological studies are necessary to trace and control this pathogen in Brazilian commercial poultry production chains.

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Molecular Detection and Characterization of Salmonella Gallinarum From Poultry Farms in Brazil

Cited by 6 publications

References 21 publications

Avaliação Da Adequação Das Boas Práticas De Fabricação E Qualidade De Carne Bovina Em Açougues

Avaliação Da Adequação Das Boas Práticas De Fabricação E Qualidade De Carne Bovina Em Açougues

Fowl Typhoid: Present Scenario, Diagnosis, Prevention and Control Measures

Emergence, Dissemination and Antimicrobial Resistance of the Main Poultry-Associated Salmonella Serovars in Brazil

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