Molecular detection of bovine leukemia virus in peripheral blood of Iranian cattle, camel and sheep

Nekoei, Shahin; Hafshejani, Taghi Taktaz; Doosti, Abbas; Khamesipour, Faham

doi:10.1515/pjvs-2015-0091

Cited by 24 publications

(16 citation statements)

References 11 publications

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“…Viruses are one of the main causes of health problems, of which bovine leukemia virus (BLV) is one of the five agents considered most significant for cattle. In Colombia reported a seroprevalence per animal of 42.7% [1], which agreed with the reported worldwide with a range between 4 and 90% [2][3][4][5][6][7][8][9]. The disease is characterized by asymptomatic passage in 70% of infected animals, with persistent lymphocytosis developing in 30% and leukemia in 5% [10][11][12][13][14].…”

Section: Introductionsupporting

confidence: 75%

Masked Genotype 6 of Bovine Leukemia Virus Is Found in Colombian Cattle

Corredor-Figueroa

Olaya-Galán

Salas

et al. 2019

Preprint

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Background Viral diseases such as bovine leukosis are a major cause of health problems associated with economic losses in the livestock industry worldwide. The prevalence of bovine leukosis ranges between 4% and 90%, and this disease is considered endemic. The etiological agent is bovine leukemia virus (BLV). Ten BLV genotypes have been reported based on analysis of complete or partial envelope gene sequences, of which genotype 1 is the most prevalent. However, the genetic variability of BLV variants circulating in Colombia remains unexplored. Therefore, in the present study, we analyzed blood samples collected from 289 cows distributed in 75 farms across the country. PCR amplification of env, gag and tax gene segments was performed. The obtained amplicons were sequenced and then subjected to phylogenetic analyses. Results A total of 62% of the cows present at 92% of the farms were BLV-positive. Genotype 1 was exclusively detected by env and gag gene segments when analyzed using previously reported primers. However, tax gene analysis revealed circulation of genotype 6 variants, which were also detected based on env gene analysis with newly designed primers. These results indicate that current genotyping approaches based on partial env sequencing may bias BLV genetic variability approaches and underestimate the diversity of the detected BLV genotypes. Conclusions This report is the first molecular and epidemiological study of BLV conducted in Colombia and contributes to the global epidemiology of the virus; it also reinforces the great impact of BLV on the country’s livestock and thus is a useful resource for farmers and government entities.

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Section: Introductionsupporting

confidence: 75%

Masked Genotype 6 of Bovine Leukemia Virus Is Found in Colombian Cattle

Corredor-Figueroa

Olaya-Galán

Salas

et al. 2019

Preprint

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“…• Ovis aries (domestic sheep) (Green et al, 1988;Pannwitz et al, 1988;Kunakov and Abakin, 1993;Nekoei et al, 2015),…”

Section: Susceptible Animal Speciesmentioning

confidence: 99%

“… Ovis aries (domestic sheep) (Green et al., ; Pannwitz et al., ; Kunakov and Abakin, ; Nekoei et al., ), Vicugna pacos (Huacaya alpaca) (Lee et al., ). …”

Section: Assessmentmentioning

confidence: 99%

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): enzootic bovine leukosis (EBL)

More¹,

Bøtner²,

Butterworth

et al. 2017

EFS2

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Enzootic bovine leucosis (EBL) has been assessed according to the criteria of the Animal Health Law (AHL), in particular criteria of Article 7 on disease profile and impacts, Article 5 on the eligibility of EBL to be listed, Article 9 for the categorisation of EBL according to disease prevention and control rules as in Annex IV and Article 8 on the list of animal species related to EBL. The assessment has been performed following a methodology composed of information collection and compilation, expert judgement on each criterion at individual and, if no consensus was reached before, also at collective level. The output is composed of the categorical answer, and for the questions where no consensus was reached, the different supporting views are reported. Details on the methodology used for this assessment are explained in a separate opinion. According to the assessment performed, it is inconclusive whether EBL can be considered eligible to be listed for Union intervention as laid down in Article 5(3) of the AHL because there was no full consensus on the criteria 5 B(i) and 5 B(iii). Consequently, since it is inconclusive whether EBL can be considered eligible to be listed for Union intervention as laid down in Article 5(3) of the AHL, then the assessment on compliance of EBL with the criteria as in Sections 4 and 5 of Annex IV to the AHL, for the application of the disease prevention and control rules referred to in points (d) and (e) of Article 9(1), and which animal species can be considered to be listed for EBL according to Article 8(3) of the AHL is also inconclusive.

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“…Epidemiological studies in Japan revealed varying levels of BLV prevalence throughout the country, based on different detection methods [ 83 , 134 – 136 ], and BLV infection rates of 40.9% of dairy and 28.7% of beef cattle, with infection rates in animals over 2-years-old reaching 78% in dairy herds and 69% in beef cattle herds [ 136 ]. Less than 6% of cattle were infected with BLV in Mongolia (3.9%) [ 77 ], Cambodia (5.3%) [ 137 ], and Taiwan (5.8%) [ 48 ], while a serological survey in Iran revealed that the prevalence of BLV was between 22.1% and 25.4% in that country [ 138 , 139 ]. Lee et al [ 102 ] demonstrated an average prevalence of BLV of 58.7% in Thailand, reaching maxima of 87.8% and 100% of cattle when assayed using PCR and ELISA, respectively.…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and genetic diversity of bovine leukemia virus

Polat

Takeshima

Aida

2017

Virol J

152

154

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Bovine leukemia virus (BLV), an oncogenic member of the Deltaretrovirus genus, is closely related to human T-cell leukemia virus (HTLV-I and II). BLV infects cattle worldwide and causes important economic losses. In this review, we provide a summary of available information about commonly used diagnostic approaches for the detection of BLV infection, including both serological and viral genome-based methods. We also outline genotyping methods used for the phylogenetic analysis of BLV, including PCR restriction length polymorphism and modern DNA sequencing-based methods. In addition, detailed epidemiological information on the prevalence of BLV in cattle worldwide is presented. Finally, we summarize the various BLV genotypes identified by the phylogenetic analyses of the whole genome and env gp51 sequences of BLV strains in different countries and discuss the distribution of BLV genotypes worldwide.

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Molecular detection of bovine leukemia virus in peripheral blood of Iranian cattle, camel and sheep

Cited by 24 publications

References 11 publications

Masked Genotype 6 of Bovine Leukemia Virus Is Found in Colombian Cattle

Masked Genotype 6 of Bovine Leukemia Virus Is Found in Colombian Cattle

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): enzootic bovine leukosis (EBL)

Epidemiology and genetic diversity of bovine leukemia virus

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