Molecular Detection of Diffusely Adherent Escherichia coli Strains Associated with Diarrhea in Shiraz, Iran

Abbasi, Parvin; Kargar, Mohammad; Doosti, Abbas; Mardaneh, Jalal; Ghorbani-Dalini, Sadegh

doi:10.5812/pedinfect.37629

Cited by 12 publications

(7 citation statements)

References 30 publications

(43 reference statements)

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“…DAEC was found more often in isolates from diarrheic isolates (29/35; 82.8%) as compared to isolates from UTI isolates (6/35; 17.1%). The prevalence of DAEC ranged from about 8% (Shiraz)[ 27 ] to 9% (Tehran and Sanandaj)[ 16 ] in Iran [ Appendix 1 and Figure 3 ].…”

Section: Resultsmentioning

confidence: 99%

An overview of diarrheagenic Escherichia coli in Iran: A systematic review and meta-analysis

et al. 2019

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Section: Resultsmentioning

confidence: 99%

An overview of diarrheagenic Escherichia coli in Iran: A systematic review and meta-analysis

et al. 2019

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“…Pathogenic E. coli is divided into two subgroups according to its predilection, namely, diarrheagenic E. coli (DEC) [54,58], which is pathogenic in the digestive tract, and ExPEC [26,59], which causes systemic infections [50]. Diarrheagenic E. coli is found in the intestinal tracts of both humans and animals and is classified into eight sub-pathotypes, including EPEC [60,61], enterotoxigenic E. coli (ETEC) [62][63][64], enterohemorrhagic E. coli (EHEC) [65][66][67], enteroinvasive E. coli (EIEC) [68][69][70][71], diffusely adherent E. coli [72][73][74][75][76], enteroaggregative E. coli (EAEC) [75,[77][78][79][80][81], adherent invasive E. coli [82][83][84][85], and Stx-producing EAEC [65,77,81,86]. Diarrheagenic E. coli is transmitted through food or water contaminated with animal or human feces and causes gastroenteritis and diarrhea.…”

Section: Avian Pathogenic E Colimentioning

confidence: 99%

Occurrence, antimicrobial resistance, and potential zoonosis risk of avian pathogenic Escherichia coli in Indonesia: A review

2022

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Avian pathogenic Escherichia coli (APEC) causes avian colibacillosis or colibacillosis and is a major endemic disease of poultry worldwide, including in Indonesia. It is characterized by a black proventriculus and can damage other organs, leading to pericarditis, perihepatitis, water sacculitis, mesenteritis, and omphalitis. The APEC strain is one of the six main sub-pathotypes of the extraintestinal pathogenic E. coli (ExPEC) pathotype. The relationship between APEC and infection in humans is questionable. The previous studies have suggested poultry products, including meat and eggs, as a potential source of infection for the transmission of ExPEC disease to humans. Due to the absence of reporting of disease incidents and the lack of literature updates on this disease, it seems as if APEC does not exist in Indonesia. Since bacterial resistance is a growing problem in Indonesia, and globally, the World Health Organization issued a statement regarding the importance of assessing related factors and their control strategies. Antimicrobial resistance, especially multidrug resistance, presents a challenge when treating infectious diseases. In Indonesia, the incidence of resistance to several antimicrobials in cases of avian colibacillosis is high. In addition, avian and human extraintestinal E. coli infections present a potential zoonotic risk. Furthermore, a relationship exists between antibiotic resistance to foodborne bacteria and the occurrence of antibiotic resistance in humans, so the use of antibiotics in the poultry industry must be controlled. Therefore, the One Health strategy should be implemented to prevent the overuse or misuse of antibiotics in the poultry industry. This review aimed to increase awareness of people who are at risk of getting Avian pathogenic Escherichia coli (APEC) from poultry by controlling the spread of APEC by maintaining a clean environment and hygienic personnel in poultry farms.

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“…Previous studies have shown that developed multiplex-PCR (m-PCR) was rapid and ideal for the identification of specific genes that encoded to specific virulence factors. The target genes include: aat2 (CVD432), AggR, eae, ipaH, stx1, stx2, bfpA, elt, estA1, estA2-4, and daaD [8,9]. The aat2 encoded to dispersin protein involved in aggregation, AggR regular the gene expression to adherence factors [10,11].…”

Section: Introductionmentioning

confidence: 99%

Identification, differentiation and phylogenetic typing of diarrheagenic Escherichia coli in Iraqi infants and children

Rahem,

Hussein,

Auda

et al. 2023

Reviews and Research in Medical Microbiology

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Several traditional identification and typing methods were available depending on the phenotype of diarrheagenic Escherichia coli (DEC). Multiplex-PCR was rapid for the identification of specific genes that encoded to specific virulence factors and as a consequence the identification of the pathotypes of DEC. The aim of this study to the identification of DEC by the virulence factors genes and genotyping of clinical isolates of DEC isolated from the diarrheal stool of infants and children. Forty-eight DEC isolates had been collected from different hospitals in Baghdad city and identified by Vitek 2 system. An antibiotic sensitivity test was carried out by the VITEK-2 System method for all DEC isolates. The virulence genes amplification was used to identify DEC pathotypes and DEC was typed by Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) methods. The occurrence of virulence genes is varied according to the pathotypes. Statistically, the eae and cadA genes are significantly found in all pathotypes of DEC (P = 0.001), whereas ipaH, elt, estA1, and stx2 genes are absent in all DEC pathotypes. The most predominant of the pathotypes are belonged to enteropathogenic Escherichia coli (EPEC) especially aEPEC followed by diffusely adherent E. coli (DAEC), enterotoxigenic E. coli (ETEC), and enterohaemorrhagic E. coli (EHEC), respectively. Moreover, The DEC isolates were multidrug-resistant to most of the antimicrobials. Ticarcillin was the less effective one while Gentamicin was the more effective one and the DEC pathotypes are differ in their antibiotic resistance pattern. The ERIC typing of E. coli pathotypes showed that almost all of the isolates are located on the same cluster, the same is noticed in EPEC isolates, The DEC pathotypes are dissimilar (Simson index = 1.0) except for EPEC isolates that showed some similarity among some of them (Simson index < 1.0). This study may be the first in using ERIC typing in typing of the E. coli pathotypes. In conclusion, amplification of virulence genes is a useful tool for the identification of DEC pathotypes. The highest percentage of DEC pathotypes was aEPEC followed by DAEC, tEPEC, ETEC and the less one was EHEC, while there was no exit to enteroinvasive E. coli and enteroaggregative E. coli. Most of the DEC clinical isolates are multidrug-resistant and are different in their antibiotic resistance pattern. The most common gene of local DEC isolates was cadA and eae genes followed by daaD and bfp, whereas ipaH, elt, estA1, and stx2 are not detected in all local isolates of DEC. Most of the DEC clinical isolates have a high level of dissimilarity among DEC pathotypes clinical isolates.

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Molecular Detection of Diffusely Adherent Escherichia coli Strains Associated with Diarrhea in Shiraz, Iran

Cited by 12 publications

References 30 publications

An overview of diarrheagenic Escherichia coli in Iran: A systematic review and meta-analysis

An overview of diarrheagenic Escherichia coli in Iran: A systematic review and meta-analysis

Occurrence, antimicrobial resistance, and potential zoonosis risk of avian pathogenic Escherichia coli in Indonesia: A review

Identification, differentiation and phylogenetic typing of diarrheagenic Escherichia coli in Iraqi infants and children

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