Molecular detection of <i>Chlamydia trachomatis</i> and HPV infections in cervical samples with normal and abnormal cytopathological findings

Paula, Francisco Danilo Ferreira; Fernandes, Ana Paula; Carmo, Bianca Bianco do; Vieira, Daniela Cristina Duarte; Dutra, Míriam Santos; Santos, Caleb Guedes Miranda dos; Souza, Maria do Carmo; Andrade, Túlio César Alvim; Vágó, A; Fernandes, Paula Ávila

doi:10.1002/dc.20629

Cited by 21 publications

(16 citation statements)

References 40 publications

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“…Another important study carried out by Neves et al (2016) on asymptomatic women aged 14-25 years who used public health services in Manaus showed a prevalence of 13.1% for CT infections, whereas in women aged 20-25 years this prevalence was 10.8%, as determined by the Digene Hybrid Capture II Test (HCII CT-ID). Our results corroborate the prevalence range found in the rest of Brazil (9.1-14.3%) (de Paula et al, 2007;Oliveira et al, 2009;Benzaken et al, 2010;Piazzetta et al, 2011;Garcês et al, 2013). CT infection is asymptomatic in almost all women and, if left untreated, can progress to complications such as ectopic pregnancy, pelvic inflammatory disease (PID), and tubal infertility (Golijow et al, 2005).…”

Section: Discussionsupporting

confidence: 90%

“…The prevalence of HPV in women with normal cytology was greater than the prevalence rates reported in the last ten years that range from 13 to 35% among women with normal cytology (Fedrizzi et al, 2008;Fernandes et al, 2009;Lippman et al, 2010;Oliveira et al, 2011;Castro et al, 2011;Rocha et al, 2014;Santos-Filho et al, 2016). Similarly, the rates of occurrence described for women with premalignant and malignant cervical lesions in the last 10 years were lower than those demonstrated in this study (de Paula et al, 2007;Paesi et al, 2009;Oliveira-Silva et al, 2011;Gurgel et al, 2013;Freitas et al, 2014). The most prevalent HR-HPV types were 16, 58, 33, and 31, both in the women with normal cytology and those with premalignant and malignant cervical lesions.…”

Section: Discussioncontrasting

confidence: 61%

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Prevalence of human papillomavirus, Chlamydia trachomatis, and Trichomonas vaginalis infections in Amazonian women with normal and abnormal cytology

Costa-Lira¹,

Jacinto²,

Silva³

et al. 2017

Genet. Mol. Res.

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Sexually transmitted infections are an important cause of morbidity among sexually active women worldwide, and have been implicated as cofactors in the pathogenesis of cervical cancer. We investigated the prevalence of human papillomavirus (HPV), Chlamydia trachomatis (CT), and Trichomonas vaginalis (TV), and accessed the diversity of HPV in women with normal and abnormal cytology in Manaus, Brazil. We used polymerase chain reaction and HPV genotyping by direct sequencing. The chi-square test was used to calculate the absolute and relative frequencies of the categorical variables, and Fisher's test was used when P < 0.05. The level of significance was set at 5%. All statistical analyses were performed using R 2.9.0. There were statistically significant differences in age (P = 0.0395), education level (P = 0.0131), sexual partners (P = 0.0211), condom use (P = 0.0039), marital status (P < 0.0001), and pregnancy (P = 0.0003) between the normal and abnormal groups. HPV DNA was found in 36.56 and 93.88% of subjects in the normal and abnormal groups, respectively. A total of 19 genotypes were detected; HPV16 was the most common, followed by HPV58. The percentages of TV and CT DNA were 18.04 and 9.02% in the normal group, respectively. The percentages of HPV/TV and HPV/CT coinfection were 12.5% each in women with normal cytology. These findings improve our understanding of HPV, CT, and TV, and the distribution of HPV types, which may be relevant to vaccination strategies for protecting women from the north of Brazil from cervical cancers and precancerous lesions.

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Section: Discussionsupporting

confidence: 90%

Section: Discussioncontrasting

confidence: 61%

Prevalence of human papillomavirus, Chlamydia trachomatis, and Trichomonas vaginalis infections in Amazonian women with normal and abnormal cytology

Costa-Lira¹,

Jacinto²,

Silva³

et al. 2017

Genet. Mol. Res.

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“…Nevertheless, although several epidemiological studies have found a positive association between C. trachomatis-and HPV-related cervical lesions and cervical cancer, the specific relationship between infections by these micro-organisms and cervical neoplasia has not as yet been fully clarified. Moreover, the detection of C. trachomatis in HPV-positive samples has yielded contrasting results (Castle et al, 2003;de Paula et al, 2007;Lehtinen et al, 2011;Luostarinen et al, 2013;Naucler et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

HPV genotypes distribution in Chlamydia trachomatis co-infection in a large cohort of women from north-east Italy

Seraceni

Campisciano

Contini

et al. 2016

Journal of Medical Microbiology

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Human papillomavirus (HPV) and Chlamydia trachomatis are pathogens with oncogenic potential associated with persistent infections. Epidemiological data on C. trachomatis infection status, C. trachomatis/HPV co-infection and the relationship between HPV genotypes in Italian women are only preliminary. The aim of the present study was to characterize the relationship between HPV genotypes and C. trachomatis in an extending cohort of asymptomatic immunocompetent women from an area of north-east Italy. A retrospective study was conducted using Luminex technology on cervical swabs from asymptomatic immunocompetent women, comprising 921 attending the prevention centre for the Cervical Cancer Program and 6214 who had been referred to the Sexually Transmitted Infections Center, with clinical indications of HPV and C. trachomatis infections. A quantitative real-time PCR was performed to assess chronic C. trachomatis infection by heat-shock protein 60 (Hsp60) gene expression. The overall prevalence of the investigated pathogens was 39 % (359/921) for HPV and 4 % (251/6214) for C. trachomatis. The Hsp60 gene was detected in 57 % of the women infected with C. trachomatis. HPV co-infection was present in 58 % of C. trachomatis-infected women. A high prevalence of co-infection was found in women with chronic C. trachomatis infection (68 %, P50.0002), especially in women j25 years (72 %) where HPV multiple infections were found in 78 % (P50.022). HPV genotype distribution showed that uncommon low-risk genotypes were associated with C. trachomatis. These results indicate a high frequency of co-detection of multiple HPV genotypes in chronically infected young women and suggest that the expression of the C. trachomatis Hsp60 gene may favour HPV infection.

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“…We found that the frequency of cellular changes, including RCC, was significantly greater in Chlamydia positive women than in uninfected women (74% versus 31%), consistent with previous reports. However, there are several studies showing no association or even a negative correlation between Chlamydia infection and the development of cervical lesions [16, 17, 23]. Thus, the role of Chlamydia infection as a risk factor in the development of cervical lesions is still controversial.…”

Section: Discussionmentioning

confidence: 99%

Cervical Cytopathological Findings in Korean Women withChlamydia trachomatis,Mycoplasma hominis, andUreaplasma urealyticumInfections

Choi

Roh

2014

The Scientific World Journal

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This is to investigate the cervical cytological abnormalities associated with Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum infections on routine screen. A total of 714 subjects who had undergone cervical Pap smears and concomitant analyses for cervical infections were included by a retrospective search. The frequencies of reactive cellular change (RCC) and squamous epithelial abnormalities were significantly higher in Chlamydia positive subjects than in uninfected subjects (P < 0.001). Of the 124 subjects tested for M. hominis, M. genitalium, and U. urealyticum, 14 (11%) were positive for M. hominis and 29 (23%) were positive for U. urealyticum. Squamous abnormalities were more frequent in subjects with Ureaplasma infections than in uninfected subjects (24% versus 8%). Taking together these findings, C. trachomatis and U. urealyticum may have a causal role in the development of cervical epithelial changes, including RCC. Thus, extra awareness is warranted in cervical screening of women with Chlamydia or Ureaplasma infections.

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Molecular detection of Chlamydia trachomatis and HPV infections in cervical samples with normal and abnormal cytopathological findings

Cited by 21 publications

References 40 publications

Prevalence of human papillomavirus, Chlamydia trachomatis, and Trichomonas vaginalis infections in Amazonian women with normal and abnormal cytology

Prevalence of human papillomavirus, Chlamydia trachomatis, and Trichomonas vaginalis infections in Amazonian women with normal and abnormal cytology

HPV genotypes distribution in Chlamydia trachomatis co-infection in a large cohort of women from north-east Italy

Cervical Cytopathological Findings in Korean Women withChlamydia trachomatis,Mycoplasma hominis, andUreaplasma urealyticumInfections

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